EMAGE mouse gene expression spatial database

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EMAGE:12639

Spdef SAM pointed domain containing ets transcription factor ( MGI:1353422)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011402. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011402_01	euxassay_011402_02	euxassay_011402_03	euxassay_011402_04

euxassay_011402_05	euxassay_011402_06	euxassay_011402_07	euxassay_011402_08	euxassay_011402_09

euxassay_011402_10	euxassay_011402_11	euxassay_011402_12	euxassay_011402_13	euxassay_011402_14

euxassay_011402_15	euxassay_011402_16	euxassay_011402_17	euxassay_011402_18	euxassay_011402_19

euxassay_011402_20	euxassay_011402_21	euxassay_011402_22	euxassay_011402_23	euxassay_011402_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12639_wholemount_strong.wlz

12639_wholemount_moderate.wlz

12639_wholemount_weak.wlz

12639_wholemount_possible.wlz

12639_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12639_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

spotted

strong expression: see section 10 11 weak expression: see section 09

diencephalon roof plate

strong

regional

strong expression: see section 14

stomach

moderate

regional

moderate expression: see section 05 06 07 08 09 10 weak expression: see section 03 04

rectum

moderate

regional

moderate expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30109
Entity Detected:	Spdef, SAM pointed domain containing ets transcription factor ( MGI:1353422)
Sequence:	sense strand is shown >T30109 CGACGATAGCAGCTGGGTCGCCAAAGTCCCCGAGGCCCGTGCCGGGGAGGACCACCCGGAGGAGCCCGAG CAGTGTCCCGTCATTGACAGCCAGGCCTCTGGGAGCACGTTGGATGAGCACTCGCTAGAGCAGGTGCAAT CGATGGTTGTGGGCGAGGTCCTGAAAGATATTGAGACGGCCTGCAAGCTTCTGAACATCACAGCAGACCC TGGGGACTGGAGCCCTGGTAACGTGCAGAAGTGGCTTTTATGGACAGAACACCAGTACCGGCTGCCTCCA GCAGGCAAGGCCTTCCAGGAGCTGGGCGGTAAGGAGCTGTGCGCCATGTCCGAGGAACAGTTCCGTCAGC GTGCACCCTTGGGTGGGGATGTACTGCATGCCCACCTGGACATCTGGAAGTCAGCGGCCTGGATGAAGGA GAGGACCTCGCCTGGGACCCTTCACTACTGCGCCTCCACCAGCGAGGAGGGCTGGACGGATGGTGAGGTG GACTCGTCGTGCTCCGGGCAGCCCATTCACCTGTGGCAGTTCCTGAAAGAACTGCTGCTCAAGCCCCACA GCTATGGCCGCTTCATCCGCTGGCTCAACAAGGAGAAAGGCATCTTCAAAATTGAGGACTCAGCACAGGT GGCCCGACTGTGGGGTGTGCGCAAGAACCGGCCAGCCATGAACTATGATAAACTAAGCCGCTCCATCCGC CAGTATTACAAGAAGGGCATCATTCGTAAACCCGACATCTCTCAGCGCCTTGTCTACCAATTTGTGCATC CAGTCTGAGAGCCACAGAGACCAGAGGCCTACAACCTGCCCCAGGCAGCCACTCTCTGGTTGGCCTGGTC CTCTCTGCTCACTCTGAATTCAGGGGCTGCTGGTATCCCAGAACCCAAGGTCCCAGATAGACAGCCACTG ATCTAGGGATACACATGAGCTCTCTGGGTCATACACAGGCCCCAGGAAGAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4218741), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 16710. Forward Primer - name:016710_F_IRAV24-27_C03_Spdef, sequence:CGACGATAGCAGCTGGGT; Reverse Primer - name:016710_R_SP6_IRAV24-27_C03_Spdef, sequence:GATCTTCCTGGGGCCTGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12641 same embryo
	EMAGE:12640 same embryo
	EMAGE:12643 same embryo
	EMAGE:12642 same embryo
	EMAGE:12638 same embryo
	EurExpress:euxassay_011402 same experiment
	MGI:4828406 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source