EMAGE mouse gene expression spatial database

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EMAGE:12671

Prom1 prominin 1 ( MGI:1100886)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017721. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017721_01	euxassay_017721_02	euxassay_017721_03	euxassay_017721_04

euxassay_017721_05	euxassay_017721_06	euxassay_017721_07	euxassay_017721_08	euxassay_017721_09

euxassay_017721_10	euxassay_017721_11	euxassay_017721_12	euxassay_017721_13	euxassay_017721_14

euxassay_017721_15	euxassay_017721_16	euxassay_017721_17	euxassay_017721_18	euxassay_017721_19

euxassay_017721_20	euxassay_017721_21	euxassay_017721_22	euxassay_017721_23	euxassay_017721_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12671_wholemount_strong.wlz

12671_wholemount_moderate.wlz

12671_wholemount_weak.wlz

12671_wholemount_possible.wlz

12671_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12671_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4232
Entity Detected:	Prom1, prominin 1 ( MGI:1100886)
Sequence:	sense strand is shown >T4232 GTCCAATCAGTGCGCTCAGACTCAGAGCCCTAGGCTCCTGCTCTTTAAATTACCGAGCCTTGTGGAGACC CCGGCACCTGGCCTTAAGCTCAGCCCTGAGGATGGTACTTTGAGTGAATGACCACCTTGGAGACCGTTCT TCTGTTTCCCTTGTTACCAGCCAGGAGGCAGAAGAGTCCACCGGTCCAGGAAAGACCCATTTCCCTTGAG TTTCCAGAAAGTACCTCATGCTTGAGAGATCAGGCCAACAACTATGGCTCTCGTCTTCAGTGCCCTGCTG TTACTGGGGCTGTGTGGAAAGATCTCTTCAGAAGGTCAGCCTGCATTCCATAACACTCCTGGGGCTATGA ATTATGAATTGCCTACCACCAAATATGAGACCCAAGATACCTTCAATGCTGGGATTGTTGGCCCTCTCTA CAAAATGGTGCACATCTTCCTCAACGTGGTCCAGCCGAATGACTTCCCTCTAGATTTGATCAAAAAACTC ATACAGAACAAGAACTTTGACATCTCAGTTGATTCCAAGGAGCCAGAAATCATAGTCTTGGCTCTGAAGA TTGCCCTCTATGAGATCGGAGTCCTTATCTGCGCCATCCTGGGACTGCTGTTCATTATCCTCATGCCTCT GGTGGGCTGCTTCTTTTGTATGTGCCGTTGCTGCAACAAATGCGGCGGAGAGATGCACCAGCGGCAGAAG CAGAATGCGCCATGCAGGAGGAAGTGCTTGGGCCTCTCCCTCCTGGTGATTTGTCTGCTCATGAGCCTTG GCATTATATATGGCTTTGTGGCTAACCAGCAGACCAGGACTCGGATCAAAGGGACCCAGAAACTGGCAAA GAGCAATTTCAGAGACTTTCAAACACTCCTGACTGAAACACCAAAGCAAATTGACTATGTAGTGGAGCAG TACACCAACACCAAGAACAAGGCATTCTCAGACCTGGATGGCATCGGCTCCGTGCTGGGAGGCAGAATAA AGGACCAACTAAAACCCAAAGTAACTCCTGTCCTCGAAGAGATTAAGGCCATGGCGACAGCCATCAAACA GACCAAGGATGCCCTGCAGAACATGAGCAGCAGCCTGAAAAGTCTCCAAGATGCAGCCACCCAGCTCAAT ACCAACCTGAGCTCTGTGAGAAACAGCATCGAGAATTCGCTCAGCAGCAGTGACTGTACCTCAGATCCAG CCAGCAAGATCTGCGATAGCATCAGACCAAGCCTAAGCAGTCTGGGGAGCAGCCTCAATTCAAGTCAGCT CCCATCAGTGGATAGAGAACTCAACACTGTTACTGAAGTCGACAAAACTGATCTGGAGAGCCTCGTCAAA AGGGGGTATACGACAATTGATGAAATACCCAATACAATACAAAACCAAACTGTGGATGTCATCAAAGACG TCAAAAATACCTTGGACTCCATTAGCTCCAACATTAAGGACATGAGCCAAAGTATTCCTATTGAGGATAT GCTGTTACAGGTCTCCCATTACCTTAATAACAGCAACAGATACTTAAACCAGGAGCTGCCCAAGCTGGAA GAATATGACTCGTACTGGTGGCTGGGTGGCTTGATTGTCTGCTTTCTGCTGACTCTCATTGTGACCTTCT TTTTCCTGGGCTTGCTGTGTGGTGTGTTTGGCTATGACAAGCATGCCACCCCAACTAGAAGAGGCTGTGT GTCCAACACTGGAGGCATCTTCCTCATGGCTGGGGTTGGATTCGGCTTCCTTTTTTGCTGGATATTGATG ATCCTTGTGGTTCTTACGTTTGTTGTTGGTGCAAATGTGGAAAAGTTGCTCTGCGAACCTTATGAAAACA AGAAATTATTACAGGTTTTGGACACTCCCTATCTGCTCAAGGAACAATGGCAATTTTATCTTTCTGGCAT GCTATTCAATAACCCAGACATTAACATGACCTTTGAGCAAGTCTACAGGGATTGCAAAAGAGGTCGAGGT ATATATGCTGCTTTTCAGCTTGAGAATGTCGTCAACGTCAGTGATCATTTCAACATTGACCAGATTTCTG AAAACATAAATACGGAGTTGGAAAACCTGAATGTGAACATTGATAGCATTGAACTGTTGGATAACACAGG AAGGAAGAGCCTCGAGGACTTTGCACATTCTGGGATAGATACAATCGATTATTCCACATACTTGAAGGAG ACTGAGAAATCCCCTACTGAAGTGAATCTGCTGACATTTGCCTCTACCCTGGAAGCAAAAGCAAACCAGT TGCCTGAAGGAAAGCTGAAACAGGCCTTCTTACTGGATGTACAGAATATAAGAGCCATCCACCAGCATCT CCTCCCTCCTGTGCAGCAATCACTGAAATTTGTGAGGGTGAGGAATACGTTAAGACAAAGTGTCTGGACC CTCCAGCAAACAAGCAACAAGTTGCCGGAGAAAGTGAAGAAGATCCTTGCCTCTTTGGACTCTGTTCAGC ATTTCCTCACCAATAACGTTTCCCTCATCGTTATCGGGGAAACGAAGAAGTTTGGGAAAACAATACTAGG CTACTTTGAACATTATCTGCACTGGGTCTTTTATGCCATCACAGAGAAGATGACATCCTGCAAACCCATG GCCACCGCGATGGACTCTGCTGTTAATGGCATTCTGTGTGGCTATGTTGCGGACCCTCTGAATTTGTTCT GGTTCGGCATAGGGAAAGCCACGGTGCTCTTACTTCCGGCTGTAATCATTGCTATCAAGCTGGCCAAGTA CTATCGCAGGATGGATTCAGAGGATGTATACGACGACCCGTCTCGATACTGACAACTGGAGTTGAAGCTG CTTGAACAACAAGATAGTCAACATGGAAAGCATCACAGATTTTGGATAGTTTCTGAGTCTTCTAGAACGT TCCAAGTGCAGAAGAAACCTGGTGGAGACTCAGGCGGGCACTAGGAACATGGCATCAGTGGTCTTAGGGT AGCACTTTGTCAGGAATGAACAGTCATCATGGTTATAATCCACATATCCATTGCAACTCATGAATGATTC TCTCCTGTTTTGTTTTTAACTTTTCTTTTTACACTGATTTTCTATTTAGACACTAAAACATATAGGGGTG CTTATTCCCCCTGGATACATTTACCTGTGAACCAGCTATTCCGGTGTCATAGCTGGGTACCTAACTTACT TCCATATGTGAAGTGTGCTAAACACAAACCAGTTTACAGAAGAGATGTATTTTGTGTATAGTAAACTGTA TATATACCCTTTTACCACAGTCAGTTTTTTAAACAAATGAATACTCTAGATTTTTCTTCTAAATGAGGTT ACTGTTGGGGTGGTTGTGACCTAGTGATGCTGTAGAAAGGAGTCTGCATTCACTAAAAGTGTGTCAACCT AGAGCAGGCAATGCCCTTCCTTGTGGATTTCTGTCTGCTCGTTTTGGAGCTACCTGCGGTTTAGAAATAG AATTCAAGAACAATCACGGAGTTTCCCACTTGATGCCACTGCCAAAGTCAGAACAAGGGATCTTGAGAGA AGGAACTGTCGCTCAGCTGGGAGCGGAATCATTATCGCAATCACAGGTCCTGGTTCACAGTTTAGTGGCA CTCTCTGGTTTGTAAGAATGGGCATTACGTTCAGTGTCATCTGGTCATCTGTGATGTGTGTCATCAGCCT GTCCTGATGTTGAGATTTAAAATAAAGCATGAATGAACAGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4502359 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4502359 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12673 same embryo
	EMAGE:12672 same embryo
	EMAGE:12674 same embryo
	EurExpress:euxassay_017721 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source