EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12674

Spock3 sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3 ( MGI:1920152)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017723. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017723_01	euxassay_017723_02	euxassay_017723_03	euxassay_017723_04

euxassay_017723_05	euxassay_017723_06	euxassay_017723_07	euxassay_017723_08	euxassay_017723_09

euxassay_017723_10	euxassay_017723_11	euxassay_017723_12	euxassay_017723_13	euxassay_017723_14

euxassay_017723_15	euxassay_017723_16	euxassay_017723_17	euxassay_017723_18	euxassay_017723_19

euxassay_017723_20	euxassay_017723_21	euxassay_017723_22	euxassay_017723_23	euxassay_017723_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12674_wholemount_strong.wlz

12674_wholemount_moderate.wlz

12674_wholemount_weak.wlz

12674_wholemount_possible.wlz

12674_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12674_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4223
Entity Detected:	Spock3, sparc/osteonectin, cwcv and kazal-like domains proteoglycan 3 ( MGI:1920152)
Sequence:	sense strand is shown >T4223 AGCAGCTCCAGCAAGAAGCGAGTTGCCAGAGCCGGGCTGCTTTTCCATCAGGAGCTGCGAGCTCCGTGCC CGCTCTCAACAAGATGCTCAAGGTGTCAGCCTTATTGTGTGTGTGTGCAGCGGCTTGGTGCAGCCAGACT CTTGCAGCTGCCGCGGCGGTGGCTGTGGCCGGGGGGCGGTCGGACGGCGGTAATTTTCTGGACGAAAAAC AATGGCTCACGACAATCTCTCAGTATGACAAGGAAGTCGGACAGTGGAACAAATTCCGAGACGAAGTAGA GGATGATTATTTCCGCACTTGGAATCCAGGAAAACCATTTGATCAAGCTTTAGATCCTGCTAAGGACCCA TGCTTAAAGACGAAATGCAGCCGCCACAAAGTTTGCATTACGCAGGATGCTCAGACTGCACTGTGTATCA GTCACCGGAGGCTCACACACAGTATGAAAGAAGTAGGAGGAAGCCATAAGCAATGGAGAGGTCTCCCGTC ATCTACCTGCAAGCCATGCCCTATCGCCTATGCCAGCCCAGTCTGTGGTTCTGATGGGCATTCCTACTCT TCACAGTGCAAACTAGAATATCAGGCATGTGTCTTAGGAAAACAGATCTCTATCAAATGTGAAGGACGTT GCCCATGTCCCTCCGATAAGTCCATGAACATAGGCAGAAATGTTAAAAGAGCCTGCAGTGACCTGGAATT CAGAGAAGTGGCAAACAGACTGCGAGACTGGTTCAAGGCCCTTCATGAGAGTGGAAGCCAGAACAAGAAG ACAAAAGCATTGCTGAGGCCTGAGAGAAGCAGATTTGATACCAGTATTTTGCCAATTTGCAAGGATTCAC TTGGCTGGATGTTTAATAGACTTGATACAAACTATGACCTGCTATTGGACCAGTCAGAGCTTGGGAGCAT ATACCTTGATAAGAATGAGCAGTGCACCAAGGCGTTCTTCAATTCTTGTGACACTTACAAGGACAGTTTG ATATCTAATAATGAATGGTGCTACTGTTTCCAGAGACAGCAAGACCCACCTTGCCACACGGAGCTCAGTA ACATTCAGAAGCGACAGGGGATAAAGAAGCTCCTAGGACAGTACATTCCACTCTGTGATGAAGATGGTTA CTACAAACCAACACAATGCCATGGCAGTGTAGGGCAGTGCTGGTGTGTTGACAGATATGGAAATGAAGTT GTTGGATCAAGAATAAATGGTGTTGCAGATTGTGCTATAGACTTTGAGATATCTGGAGATTTCGCAAGTG GAGATTTTCGTGAATGGACTGATGATGAAGGTGAAGAAGATGACATTATGAATGATAAGGATGATATTGA AGATGATGATGAAGATGAAGGGGATGATGATGACGATGGGGATGTCCATGATGGATATATTTGATCAGTA ACAGCTAATGTCAATAAATTCTACATTTTTAATATTTACAAAATAATAGCATATTGAGAATTTCCTTCTT ATCCCTAAACAAAGTGGTTCTAAACCTGACATATATTTTGTATAATTATTTCAAATATTGAAGCTAAAAT CATAGAACTTGATGTTAAATTAGAATTGTTTAATTTCAGTTTTCATATGCCTTAGAAAAAAAGAAAACAC ATATGGGTTGCAGCCTGAATATAGAAAGTTGTAAAGATTACTATAGCAAAAATTTCATAAGAAAATACTA TGTAATTTTTCCACAAACAAAATAACAGTGAATGTGTGCTTGGGATCATGTGTGTTGCTTTTATGACAGA TTGTATAAGTTAAATTTAAAATAGGTAACTAATGACCTGAACCACAATAATTTCATCGATTAACTCATGA TGTTTACTTCAATTCAAATTTGGCATCTTCCCAAGACTTATAGTTGGTATTTTAAGAAAAACAACTCATG GTAAGAGAAAGTGGTAATCTTTTCAATCGTGAAGTTATACTGGGTATGATTAAATATTATATGCAATGCA AACTATGGAAAACTTTGGGTTCTGAAAGTTAAAAACTTAAGATTTTTCTTTCTACTTTTTAATTTAAATA GCATTGCATAACATGTGGATTTTTAAAAATATTCCTTGACAACCTCATTTCTCAGTTTCTCTCTCTTTCT GAGTAGTAATCATGTATAGAATTTCAATTAGAAGGAGAAACAGACTTATTTCAACCCCTTTTTCTATAAA ATATAATTTATTTGTCCCAAGATCAATTTTGAAAATATGAACTCTCAGTTTTCATTTAGTATAAAATTTT GCATTTGATCTTGGAATCATGCCATTTTTGTTAGTGGTATTGAAATTAGAGACCATGTTGTATGGTGCCT TGCAAAAATAGAAATAGAAAGGGTTAGCATGTATATCAGTATAGAAGACTAGGCAATGTGTAAGCACACA TAATTAACAAATTATTGCTATTTAAATACTGCTAGAATGAAGAAAATGGAATATGTTTCTTTCTTTTTTT CTATTGTTACATAATCCCATATGGACTTGTTCATAATTATTGTGAAGAGTAGCATTTAAGATTACCTGTA ACGAAAGTTACTTTGTTCACTATACATCAGTTAGCATGTTAATTAATTGTGTAGACATTTTGACATACCA CCACCTTTGGGTATATCACACCACTAGTTTTGTGCTCATAATAGAAGTGGGAAAACCTGTTGAATTTTAC ACATTGGTATCTTTCACTTCAACAGTGAATTTACAGATTTCCAGGAATATCATTCATAAGAACACAAATT TGGTGAACTCTTGCCATTTAATTTATATACAGTAAATAAAAACTTTAATGTTGAACTTACATAATATTTA AGCTATAATGCATTTTCTTTTTTTTACTGTTTATGTATAGTTTACAAATAAAGAATCTTGTAACCAAAGT AAACAGTTCCTTAATGATTTTCTTGGGTATACATAATAGCATGAAGAGTTTTTAATTTATTCCTTAATTC TGCAAAATAACATACCACAACAGAGCCAATAAGTTTTTCTCAGTTTATTTTTAAGTTTTTAAACCTGATT TGTTAGTGTCTTGGGTAGAATATATTTATCTTTGTTTGTTGGTTTATTTTAATCTGTTTATTTGTCAAAA TATTGAGAAATAAATACTTTTCTTTCCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4502169 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4502169 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12673 same embryo
	EMAGE:12672 same embryo
	EMAGE:12671 same embryo
	EurExpress:euxassay_017723 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source