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EMAGE:12686

Litaf LPS-induced TN factor ( MGI:1929512)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12686 EMAGE:12686 EMAGE:12686 EMAGE:12686 EMAGE:12686
"Pseudo-wholemount" of euxassay_013657. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013657_01 euxassay_013657_02 euxassay_013657_03 euxassay_013657_04
EMAGE:12686 EMAGE:12686 EMAGE:12686 EMAGE:12686 EMAGE:12686
euxassay_013657_05 euxassay_013657_06 euxassay_013657_07 euxassay_013657_08 euxassay_013657_09
EMAGE:12686 EMAGE:12686 EMAGE:12686 EMAGE:12686 EMAGE:12686
euxassay_013657_10 euxassay_013657_11 euxassay_013657_12 euxassay_013657_13 euxassay_013657_14
EMAGE:12686 EMAGE:12686 EMAGE:12686 EMAGE:12686 EMAGE:12686
euxassay_013657_15 euxassay_013657_16 euxassay_013657_17 euxassay_013657_18 euxassay_013657_19
EMAGE:12686 EMAGE:12686 EMAGE:12686 EMAGE:12686 EMAGE:12686
euxassay_013657_20 euxassay_013657_21 euxassay_013657_22 euxassay_013657_23 euxassay_013657_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12686Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12686_wholemount_strong.wlz
12686_wholemount_moderate.wlz
12686_wholemount_weak.wlz
12686_wholemount_possible.wlz
12686_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12686_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
weak weak
regionalweak expression: see section 06 07 08 09 17 18 19
vibrissa
weak weak
regionalweak expression: see section 04 05 19 20
diencephalon lateral wall ventricular layer
weak weak
regionalweak expression: see section 11 12 14
olfactory cortex ventricular layer
moderate moderate
homogeneousmoderate expression: see section 09 10 15 16
telencephalon ventricular layer
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24
pons ventricular layer
weak weak
regionalweak expression: see section 07 08 09 15 16 17 18 19
midbrain ventricular layer
weak weak
regionalweak expression: see section 09 10 11 12 14 15 16 17 18
inner ear vestibular component
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 17 18 19 20 21
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 14 weak expression: see section 09 10 11 13
lower jaw molar
moderate moderate
regionalmoderate expression: see section 06 18 weak expression: see section 05 19
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 14 weak expression: see section 09 10 11 13
upper jaw molar
moderate moderate
regionalmoderate expression: see section 06 18 weak expression: see section 05 19
urethra of male
weak weak
regionalweak expression: see section 12 13 14
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 10 11 12 weak expression: see section 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31645
Entity Detected:Litaf, LPS-induced TN factor ( MGI:1929512)
Sequence:sense strand is shown

>T31645
AGTGCACAGAAGGGCTGCTCACACCCACCAGACACATACAGATAAGGTCACAAGTACTAAATAAAAGGCT
CACATTCCTTCCTGTCTCAGCTCCTGGCCTGTGAAGGGTCTCCTTTCTCATACAGTTTCTGGGAACTCCC
TTTGTTCATAAACACAGATGAAAGTTACTTGGCAGGGAGACATTTCTAGAATAACTTTTGTAATCACGAT
ACACCATTTCTCCATTTGCTGGTGTGTTTTTAGTTGGTTCTGTTTTAGTTTTTCACATTCAGTCCAGCTT
GTTGGCCAGGCCAGCCTCGAACTCCTAGGGGATCCTCAGTCTTCGGCTCTCAGACATGCAGGGCCCTAGC
TGTGTGCCTCTACACCCACTGGGCCTTTTGTTCTGTTTTTCTTTGACAGAGAAACAAACAGGTAATTTCA
TTGCCTGCCTATGTAGGTTTGGTGTAGTTTTGAAAGCCTTGTCTCTGTGGGGTCTCTTTGAGGGAGTTGG
GGGACCTCTATTTGAGCCCTGCTTCAGTGCCGAGAGATTGGGAGTGGAACTCCCTGAAACATGATTTCAG
TTTTTAGAGATGACCTTCTGTGGTACGGTCATCATGGTCTCCAACATCACCAGACCAAGTAGAAGGATGT
CAAAGACAAATTCAGGGCTCTTTCTAGGGTGACCACTCTGCCGTGTATAATATTGTCCCAGTCTGGCCAG
CTTTCTAGGAGGCCCTGGCTCCATCCTAAGTCTACACAGGATCTGCTGCAAATAACAGTGTACGGAGTCA
ACCTGACCCGGAACAGAAGTCTGCATGCTGGTTAAGATGCAGCCTAGCCATTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:2812674), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 12489. Forward Primer - name:012489_F_IRAV08-11_H08_Litaf, sequence:AGTGCACAGAAGGGCTGC; Reverse Primer - name:012489_R_SP6_IRAV08-11_H08_Litaf, sequence:GCAATGGCTAGGCTGCAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12685 same embryo
 EMAGE:12687 same embryo
 EMAGE:12684 same embryo
 EMAGE:12683 same embryo
 EurExpress:euxassay_013657 same experiment
 MGI:4825941 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS