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EMAGE:127

Epha4 Eph receptor A4 ( MGI:98277)
TS14 (9.0 dpc)
in situ hybridisation

Data Images
EMAGE:127 EMAGE:127
Fig 1C, Nieto et al, 1992 [PMID:1295734] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig 1B, Nieto et al, 1992 [PMID:1295734] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Corresponding brightfield and darkfeild images are shown. r3 - rhombomere3, r5 - rhombomere5, o - otocyst
Expression Pattern Description
Spatial Annotation:
EMAGE:127EMAGE:127Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
127_voxel_strong_3D_1.wlz
127_voxel_moderate_3D_1.wlz
127_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:127_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
otic placode
strong strong
rhombomere 03
strong strong
rhombomere 02
moderate moderate
rhombomere 06
moderate moderate
rhombomere 05
strong strong
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:17719
Entity Detected:Epha4, Eph receptor A4 ( MGI:98277)
Sequence:sense strand is shown

>MGI:17719
GAAGACCTATGTGGATCCTCACACTTACGAAGACCCCAACCAGGCTGTACTCAAGTTTACCACCGAGATC
CACCCATCCTGTGTGGCAAGGCAGAAGGTCATTGGAGCAGGAGAGTTTGGAGAGGTCTATAAAGGGACGC
TGAAGGCATCCTCGGGGAAGAAGGAGATACCGGTGGCCATCAAGACACTGAAAGCGGGCTACACTGAGAA
GCAGCGGGTGGACTTCCTGAGCGAGGCCAGCATCATGGGCCAGTTTAGCCACCACAATATCATCCGCCTG
GAGGCGGTGGTCTCTAAATACAAACCCATGATGATTATCACAGAGTACATGGAGAATGGAGCGCTAGACA
AGTTCCTTAGGGAGAAGGATGGTGAGTTCAGCGTACTTCAGTTGGTGGGCATGCTGAGGGGTATCG
nt 1848 - nt 2263 of NM_010139.1
Notes:The probe is described by Nieto et al, 1992 [PMID:1295734] and depicted in Fig 1a of that paper (as "probe 2")
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Age:9.0 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:section
Procedures
Staining procedure:autoradiography
General Information
Authors:Nieto et al, 1992 [PMID:1295734] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1295734] Nieto MA, Gilardi-Hebenstreit P, Charnay P, Wilkinson DG 1992 A receptor protein tyrosine kinase implicated in the segmental patterning of the hindbrain and mesoderm. Development (116):1137-50
Links:MGI:1335751 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI