EMAGE mouse gene expression spatial database

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EMAGE:12700

Sbf1 SET binding factor 1 ( MGI:1925230)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011483. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011483_01	euxassay_011483_02	euxassay_011483_03	euxassay_011483_04

euxassay_011483_05	euxassay_011483_06	euxassay_011483_07	euxassay_011483_08	euxassay_011483_09

euxassay_011483_10	euxassay_011483_11	euxassay_011483_12	euxassay_011483_13	euxassay_011483_14

euxassay_011483_15	euxassay_011483_16	euxassay_011483_17	euxassay_011483_18	euxassay_011483_19

euxassay_011483_20	euxassay_011483_21	euxassay_011483_22	euxassay_011483_23	euxassay_011483_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12700_wholemount_strong.wlz

12700_wholemount_moderate.wlz

12700_wholemount_weak.wlz

12700_wholemount_possible.wlz

12700_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12700_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24

olfactory cortex marginal layer

weak

regional

weak expression: see section 11 12 13 14 17 18 19 20

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 10 11 12 18 19

pons mantle layer

weak

regional

weak expression: see section 08 20

facial vii ganglion

moderate

regional

moderate expression: see section 06 07 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 09 10 20 21

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 19 20 21 22 23 24 weak expression: see section 11

trigeminal v nerve

weak

regional

weak expression: see section 11 19

ventral grey horn

moderate

regional

moderate expression: see section 13 14 18 19 weak expression: see section 12

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 13 14 19 20 weak expression: see section 12

neural retina

moderate

regional

moderate expression: see section 03 04 weak expression: see section 02 05

mandible

weak

regional

weak expression: see section 07 08 09 10 11 12 13 18 19 20 21 22 23 24

maxilla

weak

regional

weak expression: see section 07 08 09 10 11 18 19 20 21 22

orbito-sphenoid

moderate

regional

moderate expression: see section 04 05 06 weak expression: see section 07 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30297
Entity Detected:	Sbf1, SET binding factor 1 ( MGI:1925230)
Sequence:	sense strand is shown >T30297 AGCTGGGGCTGCTGTATGAGGAGAAGGGTGAACGCAGGGGTCAGCTGGCCTGCAAGTCAGTGTGGGAGTA TGTAGACCGGCTAAGCAAGAGGACCCCCATGTTCTACAACTACACATATGCACCCGAGGACACAGAGGTG CTGCGACCCTACAGCAATGTGTCCAATCTGAAGGTGTGGGATTTCTACACTGAGGAGACGCTGGCTGAGG GGCCCCCTTATGACTGGGAGCTGGCCCAGGGACCCCCTGAGCCTCCAGAAGAGGAACGTCCTGATGGAGG TGCTCCCCAGAGCAGGCGCCGTGTGGTATGGCCATGCTATGACAGCCGTCCTCGAGTCCAGCCTGATGCC ATCTCACGTCTGCTGGAGGAGCTGCAGCGCTTGGAGACAGAGTTAGGTCGGCCTTCTGAACGCTGGAAAG ACACCTGGGACCGGGTGAAGGCTGCTCAGCGCCTTGAAAGCCGGCAAGATGGACGTGGTACCCCCAGCTC ACTTCTGGTGTCGGCTGTGCCCCACCACCGCCGCTCGCTAGGCGTCTATCTACAGGAGGGGCCTGTGGGC TCTACTCTTAGCCTCAGCCTGGACAGTGACCAGAGCAGTGGCTCAACCACATCTAGCTCTCGGCAGGCCG CCCGGCGGAGCACCAGCACCCTGTACAGCCAGTTCCAGACAGCTGAGAGTGAGAACAGGTCCTATGAGGG CATCCTATACAAGAAGGGGGCCTTCATGAAGCCCTGGAAAGCCCGTTGGTTTGTCCTAGACAAGACCAAG CACCAGCTGCGATACTATGACCACCGAATGGACACAGAATGCAAGGGTGTCATTGACCTGGCAGAGGTGG AAGCTGTGGCACCTGGCACACCCACTATAGGTGCCCCTAAGACTGTGGATGAGAAGGCCTTCTTTGATGT GAAGACAACACGTCGCGTTTACAACTTCTGTGCCCAGGATGTGCCCTCAGCCCAGCAGTGGGTGGACCGG ATCCAGAGCTGCCTGTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4953352), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 30303. Forward Primer - name:030303_F_IRAV77-80_E14_Sbf1, sequence:AGCTGGGGCTGCTGTATG; Reverse Primer - name:030303_R_SP6_IRAV77-80_E14_Sbf1, sequence:CGACAGGCAGCTCTGGAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12698 same embryo
	EMAGE:12701 same embryo
	EMAGE:12697 same embryo
	EMAGE:12699 same embryo
	EMAGE:12702 same embryo
	EurExpress:euxassay_011483 same experiment
	MGI:4827879 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source