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EMAGE:12710

Sox10 SRY-box containing gene 10 ( MGI:98358)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12710 EMAGE:12710 EMAGE:12710 EMAGE:12710 EMAGE:12710
"Pseudo-wholemount" of euxassay_011303. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011303_01 euxassay_011303_02 euxassay_011303_03 euxassay_011303_04
EMAGE:12710 EMAGE:12710 EMAGE:12710 EMAGE:12710 EMAGE:12710
euxassay_011303_05 euxassay_011303_06 euxassay_011303_07 euxassay_011303_08 euxassay_011303_09
EMAGE:12710 EMAGE:12710 EMAGE:12710 EMAGE:12710 EMAGE:12710
euxassay_011303_10 euxassay_011303_11 euxassay_011303_12 euxassay_011303_13 euxassay_011303_14
EMAGE:12710 EMAGE:12710 EMAGE:12710 EMAGE:12710 EMAGE:12710
euxassay_011303_15 euxassay_011303_16 euxassay_011303_17 euxassay_011303_18 euxassay_011303_19
EMAGE:12710 EMAGE:12710 EMAGE:12710 EMAGE:12710 EMAGE:12710
euxassay_011303_20 euxassay_011303_21 euxassay_011303_22 euxassay_011303_23 euxassay_011303_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12710Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12710_wholemount_strong.wlz
12710_wholemount_moderate.wlz
12710_wholemount_weak.wlz
12710_wholemount_possible.wlz
12710_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12710_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
adrenal medulla
weak weak
regionalweak expression: see section 10 11 17 18 19
sublingual gland primordium
strong strong
regionalstrong expression: see section 09 16
submandibular gland primordium
strong strong
regionalstrong expression: see section 08 09 10 11 17 18 19 20
vibrissa
weak weak
regionalweak expression: see section 05 06 21 22 23
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 05 06 20 21 22
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 07 08 19
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 09 18
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 08 09 18 19
trigeminal v nerve maxillary division
weak weak
regionalweak expression: see section 06 07 08 21 22
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 09 10 11 12 17 18 19
saccule
strong strong
regionalstrong expression: see section 01 02 08 18 21 24
cochlea
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 17 18 19 20 21 22 23
utricle
strong strong
regionalstrong expression: see section 05 06 07 08 18 19 20 21
viscerocranium
weak weak
regionalExpression in the turbinate bone.
esophagus
moderate moderate
regionalmoderate expression: see section 13 14
tongue muscle
moderate moderate
regionalmoderate expression: see section 12 13 14 15
stomach
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11
hindgut
moderate moderate
regionalmoderate expression: see section 13 14
midgut
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30112
Entity Detected:Sox10, SRY-box containing gene 10 ( MGI:98358)
Sequence:sense strand is shown

>T30112
AGAAGGCTCCCCCATGTCAGATGGGAACCCAGAGCACCCCTCAGGCCAGAGCCATGGCCCCCCAACCCCT
CCAACCACCCCAAAGACAGAGCTGCAGTCCGGCAAGGCAGACCCCAAAAGGGATGGGCGCTCCTTGGGGG
AGGGCGGGAAGCCCCACATCGACTTCGGCAACGTGGACATCGGGGAGATCAGCCACGAGGTAATGTCCAA
CATGGAAACCTTTGATGTGACTGAGCTGGACCAATACCTGCCACCCAACGGGCACCCAGGCCATGTGGGT
AGCTACTCGGCAGCTGGCTATGGGCTGGGCAGTGCCCTGGCTGTGGCCAGTGGACACTCTGCCTGGATCT
CCAAGCCACCAGGTGTGGCTCTGCCCACGGTCTCGCCCCCTGGTGTGGATGCCAAAGCCCAGGTGAAGAC
AGAGACCACAGGCCCCCAGGGACCCCCACACTACACCGACCAGCCGTCCACTTCCCAGATCGCCTACACC
TCCCTCAGTCTGCCCCACTACGGCTCCGCCTTCCCCTCCATCTCACGACCCCAGTTTGACTATTCTGACC
ATCAGCCCTCAGGACCCTATTATGGCCATGCAGGCCAGGCCTCTGGCCTCTATTCAGCCTTTTCTTACAT
GGGGCCCTCCCAGCGGCCCCTCTACACTGCCATCTCTGACCCCAGCCCCTCGGGGCCCCAATCCCACAGT
CCCACACACTGGGAGCAGCCAGTATATACGACTCTATCCCGACCTTAGAGGGGCCCTGTCACCACCAGTG
CCCACAGGGCCCCGGGGCCCCTTCCCCCAGCCTGTGTGCCCTGCTCCTCATCAGCCTCAGGCTTGGCAGT
GGAGGAGGCTGCAGAGGCTGACAGCAGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3675437), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17490. Forward Primer - name:017490_F_IRAV24-27_E06_Sox10, sequence:AGAAGGCTCCCCCATGTC; Reverse Primer - name:017490_R_SP6_IRAV24-27_E06_Sox10, sequence:TGCTGCTGTCAGCCTCTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12709 same embryo
 EurExpress:euxassay_011303 same experiment
 MGI:4828387 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS