EMAGE mouse gene expression spatial database

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EMAGE:12711

Coil coilin ( MGI:104842)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013629. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013629_01	euxassay_013629_02	euxassay_013629_03	euxassay_013629_04

euxassay_013629_05	euxassay_013629_06	euxassay_013629_07	euxassay_013629_08	euxassay_013629_09

euxassay_013629_10	euxassay_013629_11	euxassay_013629_12	euxassay_013629_13	euxassay_013629_14

euxassay_013629_15	euxassay_013629_16	euxassay_013629_17	euxassay_013629_18	euxassay_013629_19

euxassay_013629_20	euxassay_013629_21	euxassay_013629_22	euxassay_013629_23	euxassay_013629_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12711_wholemount_strong.wlz

12711_wholemount_moderate.wlz

12711_wholemount_weak.wlz

12711_wholemount_possible.wlz

12711_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12711_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 11 14 weak expression: see section 15

dorsal grey horn

moderate

single cell

moderate expression: see section 12 13 14 15 weak expression: see section 09 10 11 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T522
Entity Detected:	Coil, coilin ( MGI:104842)
Sequence:	sense strand is shown >T522 GTTGGCCTACTGGATGAGCAAGATGGCAGCCTCCGAGACGGTTAGGCTACGGCTTCAATTTGACTACCCG CCGCCGGCCACTCCACACTGCACGGTCTTCTGGCTTCTGGTGGACCTGAACAGATGTCGAGTTGTCACGG ATCTCATCAGTCTCATCCGCCAGCGCTTCGGCTTCAGTTCCGGGGCGCTCCTGGGCCTCTATCTGGAAGG GGGGCTGCTGCCCCCTGCCGAGAGCGCGCGCCTGGTGAGAGACAACGACAGCCTCAGGGTTAAGTTAGAA GACCAAGGACTCCCTGAGAACCTGATAGTAAGCAATGGTGGCGACTCTAGTTTCCCATGCAGGAAAGCAA AGAAACGGGCTTTTAAGTTGATGGAGGATGAGGAGACAGATCAGGGATACAAGTCTTCGAAGAAGCACTG CAAGAGGCAGGAGGACAGCGGCCAGAATGAGAAGGCCTCGGACCTGGAAACTAAACTCCTCCCAGATGAG ACGGGGCGGACAAAGAGCAAGAAAAAGAGCAAAGTGACAGGCAGCCCA
Notes:	The probe template was PCR amplified from IMAGE:1498290 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1498290 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12712 same embryo
	EMAGE:12713 same embryo
	EurExpress:euxassay_013629 same experiment
	MGI:4823972 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source