EMAGE mouse gene expression spatial database

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EMAGE:12745

Hmcn2 hemicentin 2 ( MGI:2677838)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011593. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011593_01	euxassay_011593_02	euxassay_011593_03	euxassay_011593_04

euxassay_011593_05	euxassay_011593_06	euxassay_011593_07	euxassay_011593_08	euxassay_011593_09

euxassay_011593_10	euxassay_011593_11	euxassay_011593_12	euxassay_011593_13	euxassay_011593_14

euxassay_011593_15	euxassay_011593_16	euxassay_011593_17	euxassay_011593_18	euxassay_011593_19

euxassay_011593_20	euxassay_011593_21	euxassay_011593_22	euxassay_011593_23	euxassay_011593_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12745_wholemount_strong.wlz

12745_wholemount_moderate.wlz

12745_wholemount_weak.wlz

12745_wholemount_possible.wlz

12745_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12745_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

stomach

strong

regional

strong expression: see section 05 06 07 08 09 10 11

midgut

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

left lung

strong

regional

strong expression: see section 10 11

right lung

strong

regional

strong expression: see section 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31694
Entity Detected:	Hmcn2, hemicentin 2 ( MGI:2677838)
Sequence:	sense strand is shown >T31694 GCCCTGCAAACAGAGGAAAATGAAGTTGGCTGTCCCGAGGGCTTTGAGCCGGATGTGCAGGGAGCATTTT GTGTGGACAAGGACGAATGCTCAGGAGGTCCCAGCCCCTGCTCTCATACCTGCCGCAATGCTCCTGGCCA TTTCTCCTGCTCCTGCCCCACTGGCTTCTCCCTGGCTTGGGACCACAGGAATTGTAGAGATGTGGATGAG TGTGCGGGCGACACCCACCTCTGCCAGGAGGAACAGCGCTGTGTGAACCTGCTTGGATCCTATAACTGCC TCGCTTCCTGTAGACCTGGCTTCCGGGTAACTGCTGACGGAAGCAACTGTGAAGATGTGGATGAATGTCT AGAACAGCTGGATGAGTGTCACTACAATCAACTCTGTGAGAACACTCCAGGTGGCCACCACTGTGGTTGT CCCAGAGGGTACCGCCAACAGGGTCACAGCCTGCCTTGCCTAGACATCAACGAGTGCCTACAGCTGCCCA CACCCTGTGTCTACCAGTGTCAGAACCTCCAGGGCAGCTACCGATGCCTGTGCCCACCAGGCCAGACTCT CCTACGGGATGGCAGGACCTGTATCCCCCTGGAACGGAACCGTCAAAACATTACCATTGTCAGCCACCGG AGCCCATTTGGGCCCTGGCTGCGGTCTCGGGTACCCAGGCCCAGTAGTTCCTACCACACCTGGGTCTCCC TCCGGCCTGGTTCTGGGGCCCTGAATAGTGTGGGCCGGGCCTGGTGCCCACCTGGCTTCATAAGGCAGGA TGGCGTGTGTGCAGACCTTGATGAGTGCCGTGTTAGGAGCCTGTGTCAGCATGCCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4222782), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 37570. Forward Primer - name:037570_F_IRAV81-84_L02_BC034076, sequence:GCCCTGCAAACAGAGGAA; Reverse Primer - name:037570_R_SP6_IRAV81-84_L02_BC034076, sequence:CAGGCATGCTGACACAGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12747 same embryo
	EMAGE:12746 same embryo
	EurExpress:euxassay_011593 same experiment
	MGI:4825386 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source