EMAGE mouse gene expression spatial database

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EMAGE:12760

Fign fidgetin ( MGI:1890647)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013646. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013646_01	euxassay_013646_02	euxassay_013646_03	euxassay_013646_04

euxassay_013646_05	euxassay_013646_06	euxassay_013646_07	euxassay_013646_08	euxassay_013646_09

euxassay_013646_10	euxassay_013646_11	euxassay_013646_12	euxassay_013646_13	euxassay_013646_14

euxassay_013646_15	euxassay_013646_16	euxassay_013646_17	euxassay_013646_18	euxassay_013646_19

euxassay_013646_20	euxassay_013646_21	euxassay_013646_22	euxassay_013646_23	euxassay_013646_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12760_wholemount_strong.wlz

12760_wholemount_moderate.wlz

12760_wholemount_weak.wlz

12760_wholemount_possible.wlz

12760_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12760_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 15 16 17 18 19 moderate expression: see section 12 weak expression: see section 13

telencephalon mantle layer

moderate

regional

moderate expression: see section 03 04 05 06 weak expression: see section 07 08

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 13 14 15 17 18 weak expression: see section 16

pons mantle layer

moderate

regional

moderate expression: see section 06 07 09 10 11 12 13 14 15 weak expression: see section 16

pons marginal layer

weak

regional

weak expression: see section 19

midbrain mantle layer

strong

regional

strong expression: see section 08 09 10 11 15 16 17 18 19 moderate expression: see section 12

dorsal grey horn

moderate

regional

moderate expression: see section 08 09 10 11 13 14 15 16 17 18 weak expression: see section 12

ventral grey horn

moderate

regional

moderate expression: see section 10 weak expression: see section 11 12 13 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31975
Entity Detected:	Fign, fidgetin ( MGI:1890647)
Sequence:	sense strand is shown >T31975 CCCCTCCTCCTTCAGCATACCTGCCTTCAGGAATTCCTGCTCCCACCCCTCTGCCCCCCACTACCGTTCC TGGCTACACCTACCAAGGTCATGGTTTGACACCCATTGCACCCTCTGCTCTGACCAACAATTCAGCAAGT TCTCTCAAAAGGAAAGCTTTCTATATGGCAGGGCAAGGAGACATGGACTCCAGTTACGGAAATTACAGCT ATGGCCAACAGAGATCGACACAGAGTCCTATGTACCGAATGCCCGACAACAGCATTTCAAACTCGAATCG GGGGAATGGCTTTGACAGAAATGCTGAAACATCATCCTTAGCATTTAAGCCAACAAAGCAGCTGATGCCC TCTGAGCAGCAAAGGAAATTCAGCAGCCAGTCCAGTAGGGCTCTGACCCCTCCTTCCTATAGTACTGCTA AAAATTCATTGGGATCAAGGTCCAGTGAATCCTTTGGGAAGTATACGTCGCCAGTCATGAGTGAGCACGG AGACGACCATCGGCAGCTCCTCGCTCATCCAATACAAGGTCCCGGACTCCGTGCAGCTACCTCATCCAAC CACTCTGTGGACGAGCAACTGAAGAATACTGACACACACCTCATTGACCTGGTCACCAATGAGATCATCA CCCAAGGACCTCCTGTGGACTGGAGCGACATAGCTGGTCTTGACCTGGTGAAGGCGGTTATTAAGGAGGA GGTTTTGTGGCCAGTGCTGAGGTCAGATGCATTCAGTGGGCTGACCGCCTTACCTAGAAGCATCCTTCTC TTCGGACCTCGGGGTACAGGCAAAACACTTTTGGGCAGATGCATAGCTAGTCAGCTAGGGGCCACGTTTT TC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6409940), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59950. Forward Primer - name:059950_F_IRAV115_e08_Fign, sequence:CCCCTCCTCCTTCAGCAT; Reverse Primer - name:059950_R_SP6_IRAV115_e08_Fign, sequence:TGAAAAACGTGGCCCCTA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12759 same embryo
	EurExpress:euxassay_013646 same experiment
	MGI:4824863 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source