EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12767

C1qtnf5 C1q and tumor necrosis factor related protein 5 ( MGI:2385958)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011472. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011472_01	euxassay_011472_02	euxassay_011472_03	euxassay_011472_04

euxassay_011472_05	euxassay_011472_06	euxassay_011472_07	euxassay_011472_08	euxassay_011472_09

euxassay_011472_10	euxassay_011472_11	euxassay_011472_12	euxassay_011472_13	euxassay_011472_14

euxassay_011472_15	euxassay_011472_16	euxassay_011472_17	euxassay_011472_18	euxassay_011472_19

euxassay_011472_20	euxassay_011472_21	euxassay_011472_22	euxassay_011472_23	euxassay_011472_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12767_wholemount_strong.wlz

12767_wholemount_moderate.wlz

12767_wholemount_weak.wlz

12767_wholemount_possible.wlz

12767_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12767_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

strong

regional

strong expression: see section 05 06 07 08 10 moderate expression: see section 11 13 14 15 16 17

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 moderate expression: see section 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30312
Entity Detected:	C1qtnf5, C1q and tumor necrosis factor related protein 5 ( MGI:2385958)
Sequence:	sense strand is shown >T30312 CCACCATGAGGCCACTTCTTGCCCTTCTGCTTCTGGGTCTGGTGTCAGGCTCTCCTCCTCTGGACGACAA CAAGATCCCCAGCCTGTGTCCCGGGCAGCCCGGCCTTCCAGGCACACCAGGTCACCATGGCAGCCAAGGC CTGCCTGGCCGTGACGGCCGTGATGGCCGCGACGGTGCACCCGGAGCCCCGGGAGAGAAAGGCGAGGGCG GGAGACCGGGACTACCTGGGCCACGTGGGGAGCCCGGGCCGCGTGGAGAGGCAGGGCCCATGGGGGCTAT CGGGCCTGCGGGGGAGTGCTCGGTACCCCCACGATCAGCCTTCAGTGCCAAGCGATCCGAGAGCCGGGTA CCTCCGCCAGCCGACACACCCCTACCTTTCGACCGTGTGCTGCTAAATGAGCAGGGCCATTTCGACCCCA CTACTGGCAAGTTCACCTGCCAAGTGCCTGGCGTCTACTACTTTGCTGTGCACGCCACTGTCTACCGGGC CAGCTTGCAGTTTGATCTTGTCAAAAACGGGCAGTCCATCGCCTCTTTCTTCCAGTATTTTGGGGGGTGG CCCAAGCCAGCCTCGCTCTCAGGGGGTGCGATGGTAAGGCTAGAACCTGAGGACCAGGTGTGGGTGCAGG TGGGCGTGGGTGATTACATTGGCATCTATGCCAGCATCAAGACAGACAGTACCTTCTCTGGATTTCTCGT CTATTCTGACTGGCACAGCTCCCCAGTCTTCGCTTAAAACACAGTGAACCCGGAGCTGGCACTTGCTCCT CAGTGGAGGGTGTGACACTAACCCGCGCAGCGCATACCAGGAGGGCTGGCCCCCTGGAATATTGTGAATG ACTTAGGAAGAGAGGGAGCCACTTCCGGTCCCACTGCTGGCAATGAATGGAGACAGGCTGTCTGAGGTCA AGACAGCGTGGAGCAGTGGCTGGGTTTCTGCCCAGGAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3978387), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 29843. Forward Primer - name:029843_F_IRAV77-80_L07_C1qtnf5, sequence:CCACCATGAGGCCACTTC; Reverse Primer - name:029843_R_SP6_IRAV77-80_L07_C1qtnf5, sequence:AGTCCTGGGCAGAAACCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12769 same embryo
	EMAGE:12768 same embryo
	EurExpress:euxassay_011472 same experiment
	MGI:4823541 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source