EMAGE mouse gene expression spatial database

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EMAGE:12794

Eif1b eukaryotic translation initiation factor 1B ( MGI:1916219)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011475. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011475_01	euxassay_011475_02	euxassay_011475_03	euxassay_011475_04

euxassay_011475_05	euxassay_011475_06	euxassay_011475_07	euxassay_011475_08	euxassay_011475_09

euxassay_011475_10	euxassay_011475_11	euxassay_011475_12	euxassay_011475_13	euxassay_011475_14

euxassay_011475_15	euxassay_011475_16	euxassay_011475_17	euxassay_011475_18	euxassay_011475_19

euxassay_011475_20	euxassay_011475_21	euxassay_011475_22	euxassay_011475_23	euxassay_011475_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12794_wholemount_strong.wlz

12794_wholemount_moderate.wlz

12794_wholemount_weak.wlz

12794_wholemount_possible.wlz

12794_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12794_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 23 24 moderate expression: see section 05 06 07 08 09 10 18 19 20 21 22

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 11 12 16 17

telencephalon ventricular layer

moderate

regional

moderate expression: see section 01 02 03 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22 23 weak expression: see section 04 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30315
Entity Detected:	Eif1b, eukaryotic translation initiation factor 1B ( MGI:1916219)
Sequence:	sense strand is shown >T30315 GGTTCACGGGTTCTAAGATGAACCCAAGCGTGGCGAGTTTCTTAAATGGTTTTGTTCTCTAACTCAGTTT GGCTGCCTCGGGAGATGATTCTTTACAGTAAACGACAGACTTTGCGTTTATTTAATCATTCAGACTTCCA CTCACACCTGCATGGCTACAGAAAACACGGGGTATGTAGGCTCCTGAGTCATAAGGAAATCGCGGTGAGA TGGGAACGAAGCCCGAGTCCGTCCTAACATGTTTCCAATGGAAAAGGTTTTGTTGTGAGTGTTTGTTGTT AGTTTTATTTTCACTTGACTGTTAAATGTTTTTGTTGTTGTATTAAACCATGTATGTTGCGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4209443), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 9755. Forward Primer - name:ABA_MGC_079_02_A11_F_CAGTGGTGTGGCGACAAG_ABA_FR_079, sequence:GGTTCACGGGTTCTAAGATGA; Reverse Primer - name:_R_SP6_ABA_MGC_014_02_F07_GGTTCACGGGTTCTAAGATGAACC, sequence:GCCGCAACATACATGGTTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12797 same embryo
	EMAGE:12796 same embryo
	EMAGE:12798 same embryo
	EMAGE:12795 same embryo
	EMAGE:12793 same embryo
	EurExpress:euxassay_011475 same experiment
	MGI:4824501 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source