EMAGE mouse gene expression spatial database

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EMAGE:12799

Pea15a phosphoprotein enriched in astrocytes 15A ( MGI:104799)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011580. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011580_01	euxassay_011580_02	euxassay_011580_03	euxassay_011580_04

euxassay_011580_05	euxassay_011580_06	euxassay_011580_07	euxassay_011580_08	euxassay_011580_09

euxassay_011580_10	euxassay_011580_11	euxassay_011580_12	euxassay_011580_13	euxassay_011580_14

euxassay_011580_15	euxassay_011580_16	euxassay_011580_17	euxassay_011580_18	euxassay_011580_19

euxassay_011580_20	euxassay_011580_21	euxassay_011580_22	euxassay_011580_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12799_wholemount_strong.wlz

12799_wholemount_moderate.wlz

12799_wholemount_weak.wlz

12799_wholemount_possible.wlz

12799_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12799_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

strong

homogeneous

strong expression: see section 11 12 13 14

diencephalon lateral wall ventricular layer

strong

homogeneous

strong expression: see section 11 12 13 14

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 10 11 15 16 17

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate ventricular layer

strong

homogeneous

strong expression: see section 11 12 13 14 15

rest of cerebellum ventricular layer

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

pons ventricular layer

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

midbrain ventricular layer

strong

homogeneous

strong expression: see section 09 10 11 12 13 14 15 16 17

ventral grey horn

strong

regional

strong expression: see section 12 13 14 15 16 moderate expression: see section 17 18

spinal cord ventricular layer

strong

regional

strong expression: see section 13 14 15 16

neural retina

strong

regional

strong expression: see section 01 02 03 moderate expression: see section 04 05 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30324
Entity Detected:	Pea15a, phosphoprotein enriched in astrocytes 15A ( MGI:104799)
Sequence:	sense strand is shown >T30324 TTCCTCCACTCTTCCCCCACATCTTTATGGAAGCAGAAAGGACCTGCATTTTCCTACACTGAGGAGCTAT GGTGAAGATGAGGTATGGGAGAGATGGTTGTATCTAAAGAAAACCAGTGGGGAAGGAAGGTAAAGAGGCC ACTCAACCTCCACCTGGTAAGGGACAAAGAAAAGCCAGGACTCAGTGTTTGTATCTCTATGCTGGACTGG TTAGAAGCCAGCTTCCTGCTGTTCCCTTAGGCTGAGGTCCTGAGTGCCAATGGGCCCTCCTTATGCCCTT GTTATGGGCCTGGGATGCGGATGAGCCAGAAATCTTAATGAGAAGACCACTCGATCCTTTCCTGGTCCCA AAGATCAAACCCCAATGGAGAAGCCAGCATTTACTGTCCCCCAACCTTCTGCTCTGGAGAGACGATGCCG GGACCATGCACTACTGAGCCTGAGCTAGGGACGCAGGCAGAGACAGGCCCACTTTCTGCTCCTCAGTTTC TAAATACAGACTGTTGGATAAACTGACCTGGAGCCTAGGGAGATTGGGGGATGAGATCCTTAGGTTTTAA CCCCAACCTGCCCACAACCTACAGAGTATTGTAGGCAACCTTTCCACTCTCCAGTTTAGAATTCTCCAAG CAAGTAGTTAATTACAGTGTTTCCTTTGCACTGACCACCACCCTGATTCAATCCAGGAAGGGACTGGTAA CCTTTCTCATTTGGGTTTGTGGATGCCACACAGTCAAGTCACTAGAGTGCAGTGAGTGAACCCAGCCTCC TCCCTGTCCCAAGATGCCCTTCCCCATCTTGACCGTGCTAACTGTGTGTACATATATATTCTACATATAT GTATATTAAACCCGCACTGCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4500957), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 9965. Forward Primer - name:ABA_MGC_050_02_G10_F_GCAGCGTGTCTGATGTGG_ABA_FR_050, sequence:TTCCTCCACTCTTCCCCC; Reverse Primer - name:_R_SP6_ABA_MGC_015_02_B08_TTCCTCCACTCTTCCCCCACATCT, sequence:TGGCAGTGCGGGTTTAAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12801 same embryo
	EMAGE:12800 same embryo
	EMAGE:12802 same embryo
	EMAGE:12803 same embryo
	EurExpress:euxassay_011580 same experiment
	MGI:4827147 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source