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EMAGE:1280

Vcam1 vascular cell adhesion molecule 1 ( MGI:98926)
TS15 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:1280
Figure 2C of Kwee et al., 1995 [PMID:7539357] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: h - heart; s - somites.
Expression Pattern Description
Spatial Annotation:
EMAGE:1280Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1280_wholemount_strong_3D_1.wlz
1280_wholemount_moderate_3D_1.wlz
1280_wholemount_possible_3D_1.wlz
1280_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1280_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
heart
detected detected
regionalExpression in the myocardial cells of the heart
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1334372
Entity Detected:Vcam1, vascular cell adhesion molecule 1 ( MGI:98926)
Notes:The anti-VCAM1 antibody used in this study by Kwee et al., 1995 [PMID:7539357] is described as follows, "anti-VCAM-1, 429 (Pharmingen No. 5741)." As of 17/12/04 there are three Pharmingen catalogue numbers for 429: 553329, 550547 and 553332. Source of antigen below is taken from these.
Antibody Type:monoclonal
Raised In:rat
Supplier:Pharmingen
Catalogue Number:5741 (expired)
Specimen
Organism:mouse
Strain:CD-1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Secondary Antibody:goat anti-rat IgG
Labelled with:horse radish peroxidase
Visualisation method:DAB
General Information
Authors:Kwee et al., 1995 [PMID:7539357] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:7539357] Kwee L, Baldwin HS, Shen HM, Stewart CL, Buck C, Buck CA, Labow MA 1995 Defective development of the embryonic and extraembryonic circulatory systems in vascular cell adhesion molecule (VCAM-1) deficient mice. Development (121):489-503
Links:MGI:1334377 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI