EMAGE mouse gene expression spatial database

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EMAGE:12808

Clptm1l CLPTM1-like ( MGI:2442892)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013627. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013627_01	euxassay_013627_02	euxassay_013627_03	euxassay_013627_04

euxassay_013627_05	euxassay_013627_06	euxassay_013627_07	euxassay_013627_08	euxassay_013627_09

euxassay_013627_10	euxassay_013627_11	euxassay_013627_12	euxassay_013627_13	euxassay_013627_14

euxassay_013627_15	euxassay_013627_16	euxassay_013627_17	euxassay_013627_18	euxassay_013627_19

euxassay_013627_20	euxassay_013627_21	euxassay_013627_22	euxassay_013627_23	euxassay_013627_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12808_wholemount_strong.wlz

12808_wholemount_moderate.wlz

12808_wholemount_weak.wlz

12808_wholemount_possible.wlz

12808_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12808_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4687
Entity Detected:	Clptm1l, CLPTM1-like ( MGI:2442892)
Sequence:	sense strand is shown >T4687 CATACCTGCTGGGTCATGTACGGTATCGTCTATACCCGTCCGTGCTCGGGTGACTCCAACTGCATCCAGC CCTATCTGGCCCTGCGGCCCAAACTGCAGCTGAGTGTTTACACCACCACACGGTCCAGCCTTGGTGCAGA GAACAATGTGGACCTGATCTTGAACGTGGAAGACTTTGATGTGGATTCCAAATTTGAAAGGACAGTTAAT GTTTCTGTACCAAAGAAAACAAGAAACAATGGGACGCTGTATGCATATATTTTCCTACATCATGCTGGGA TTCTACCCTGGCAAGATGGGAAACAGGTACATGTGGTCAGTACTCTGACTACCTACATGATCCCCAAACC AGAAGAAATTAACTTGCTCACTGGGGAGTCTGCTACACAGCAACAGATAGAAGCCGAGAAGAAGCCATCA AACGCCCTGGATGAACCCGTCTCTCACTGGCGACCAAGGCTGACCCTGAATGTGATGGTGGATGACTTTG TCTTTGATGGTTCCTCCCTGCCTGCAGATGTGCATCGGTACATGAAGATGATTCAGCTTGGGAAGACAGT GCACTACCTCCCCATCCTGTTCATTGACCAGCTGAGCAACCGGGTGAAAGACCTGATGGTGATAAACCGA TCTACCACCGAGCTGCCACTCACTGTGTCCTACGACAAGATCTCATTGGGGCGGCTGCGCTTCTGGATCC ACATGCAGGATGCCGTGTACTCCCTGCAGCAGTTCGGGTTTTCAGAAAAAGATGCAGATGAATTGAAGGG AATTTTCGTAGACACCAACTTATATCTCTTAGCATTGACCTTCTTTGTTGCAGCCTTTCACCTTCTTTTT GATTTCCTGGCATTTAAAAGTGACATCAGTTTCTGGAAGAAAAAGAAGAGCATGATTGGAATGTCTACCA AAGCAGTGCTCTGGCGTTGCTTCAGCACTGTGGTCATCTTCCTCTTCCTGCTGGATGAACAGACAAGCCT GCTGGTGCTGATCCCTGCAGGTGTTGGAGCTGCCATTGAGCTTTGGAAAGTGAAGAAGGCATTGAAAATT ACAGTAGCCTGGAGGGGCTTGAGACCTGTGTTTCAGTTTGGTACCCATAGTGAATCTGAGAGGAAGACTG AGAAGTATGATGCTCAGGCCATGAAGTACTTGTCTTATCTGCTCTACCCTCTCTGTGTCGGGGGCGCTGT CTACTCACTCCTAAATATCAAGTATAAGAGTTGGTACTCCTGGCTCATCAACAGCTTTGTGAATGGTGTC TATGCCTTTGGCTTCCTCTTCATGTTGCCCCAGCTTTTTGTCAATTACAAGATGAAGTCAGTGGCACACC TGCCTTGGAAGGCCTTTACCTACAAGGCTTTTAACACCTTCATTGATGATGTCTTTGCCTTCATCATCAC CATGCCCACATCCCACCGGCTGGCCTGTTTCAGAGATGATGTGGTCTTCCTGGTCTACCTTTACCAGCGG TGGCTTTACCCTGTGGATAAGAGCAGGGTCAATGAATTTGGGGAGTCTTATGAGGAGCAGCCCAAGCGGA AGCCCCATCCAGACTGAGCATCTCGGCCCAGGCTCCCAGGCGGCACTGTACTGCACAGCACAGTTGGGCC ATGAGTATTCCTGGGGGCTTCGGGAAAATTCCTAGATACCACATCTTCTGCGTTGCCAAATCCCTCTGAG CATTTCTCAGTATCCCTGAGCACCCTCTCTTCTCGTGTTTCAGAAGGAACCATAGCACTTAACCATACAC CCCACATGAACTTGGGTCTTGGTGATTGTGCCAGTGGGCAGTCACCCAAGGGTAGCTCTCAGTGCATGAG TGTCCCATGGCAGCCAGTGGACACCTTAGCACGCAAGGGTAGGGAGCATGGTGGATTTCCACGTCAGCTT GTCCAAGTGCTCCTTATGTGGATTTCACATACACAAAAGTCGTTCTGTACGGACTCTGGGATCAAATGGC TCTTTTTCCCTTTTGTTTAAAATTTGATTGTTTAAAGCCTAATGTATGTATGTTTCTATTTTAAAATAAA TTTCTTTGGCTGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5028264 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5028264 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12809 same embryo
	EMAGE:12810 same embryo
	EurExpress:euxassay_013627 same experiment
	MGI:4823936 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source