EMAGE mouse gene expression spatial database

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EMAGE:12809

Cog3 component of oligomeric golgi complex 3 ( MGI:2450151)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013611. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013611_01	euxassay_013611_02	euxassay_013611_03	euxassay_013611_04

euxassay_013611_05	euxassay_013611_06	euxassay_013611_07	euxassay_013611_08	euxassay_013611_09

euxassay_013611_10	euxassay_013611_11	euxassay_013611_12	euxassay_013611_13	euxassay_013611_14

euxassay_013611_15	euxassay_013611_16	euxassay_013611_17	euxassay_013611_18	euxassay_013611_19

euxassay_013611_20	euxassay_013611_21	euxassay_013611_22	euxassay_013611_23	euxassay_013611_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12809_wholemount_strong.wlz

12809_wholemount_moderate.wlz

12809_wholemount_weak.wlz

12809_wholemount_possible.wlz

12809_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12809_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7137
Entity Detected:	Cog3, component of oligomeric golgi complex 3 ( MGI:2450151)
Sequence:	sense strand is shown >T7137 CCACGCGTCCGGCCCCGGTCCTCCCGGAAGTTCCCGGCTCTGCCTGTCGGGCTCCGGCTCTGCGCCGCGG AGGGGCCCAGGGCTGCGGGGATGGCGGAGGCGGCGGCCGAGCGGGACGCCCGCGAGAAGCTGTCTCTCTG GGATGGGAGACCGGACTCGATGGCGCCGCTGACAGACAGGCAGACGGACTCGGTGCTGGAGCTCAAGGCG GCCGTGGAGAACCTGCCGGTGCCCGCCGAGCTCCCAATTGAAGATGTATGCAGCTTAGCATCCCAGTCGC TGCCCATTGAGCTGACTGCTGTAGTGCCTGACTCTACAGAAGACATCCTGTTAAAGGGCTTCACTTCCTT AGGAATGGAAGAAGAAAGGATTGAGACTGCGCAGCAGTTTTTCTCATGGTTTGCAAAACTGCAAACTCAG ATGGATCAGGATGAAGGAACTAAATATAGACAGATGAGGGATTACTTGTCTGGGTTTCAGGAACAGTGTG ATGCTATACTGAATGATGTGAACAGTGCTCTGCAGCATCTGGAGTCGTTGCAGAAACAGTATCTTTTTGT GTCCAATAAGACAGGAACTCTTCATGAAGCCTGTGAACAGCTCCTAAAAGAACAGTCGGAGCTTGCTGAT CTTGCTGAGCACATCCAACAGAAGCTGTCTTATTTTAATGAGTTGGAGACGATTAACACAAAATTGAATT CCCCTACGTTGTCTGTGAATAGTGAAGGATTTATCCCTATGTTGGCCAAGTTAGATGATTGTATAACATA TATTTCATCTCATCCTAATTTTAAAGATTATCCTGTATACTTACTGAAGTTTAAACAATGTCTTTCTAAA GCTTTGCACCTCATGAAGACATATACTGTGAACACACTGCAAACTCTCACAAATCAGTTACTAAAAAGGG ACCCCTCATCAGTCCCTAATGCAGACAATGCCTTCACACTATTTTATGTGAAATTTCGAGCCGCTGCCCC TAAAGTCAGAACTCTAATTGAACAGATAGAGCAACGATCTGAAAAGATACCCGAATACCAGCACCTTCTC AATGACATCCACCAGTGTTACCTTGATCAGCGGGAGCTCCTTTTGGGCCCAAGTATTGCCTACACTGTCA CAGAGTTAACCAGCCAGAACAACAGAGACCATTGTGCCTTGGTTCGCAGTGGCTGTGCCTTTATGGTCCA TGTCTGCCAAGATGAGCACCAACTTTACAATGAATTTTTCACAAAGCCAACATCAAAATTAGATGAGCTA TTGGAGAAATTGTGCGTGTCGTTGTATGATGTGTTCCGGCCTTTGATCATCCATGTCATTCACTTAGAGA CTTTATCGGAGCTCTGTGGGATTCTTAAAAACGAAGTGCTTGAAGACCACGTACAGCACAACGCTGAACA ACTGGGGGCATTTGCAGCTGGAGTAAAGCAGATGTTGGAAGATGTACAAGAGAGGCTTGTGTATCGAACC CATATCTACATTCAGACGGACATCACAGGCTACAAACCAGCGCCTGGAGATCTGGCATATCCTGATAAGC TAGTCATGATGGAGCAAATCGCACAGAGTTTAAAGGATGAGCAGAAGAAGGCGCCTTCAGAAGCTTCCTT TTCAGATGTACGGTTAGAAGAAGGAGAAGCCAGTGGTTTAAGAAAGTCTGGTTCAACAGACTCCCTCAAT CCTAGACCACAGACGACGATCTCTCCAGCAGATCTTCACGGGATGTGGTATCCCACAGTTCGAAGGACAC TTGTCTGTCTCTCCAAATTATATAGATGCATAGATAGGGCGGTGTTCCAAGGATTATCACAGGAAGCCTT GTCTGCCTGCATACAGTCCTTGCTAGGAGCATCAGAGTCTATCAGCAAAAACAAGACTCAGATTGATGGA CAACTTTTCTTAATTAAACACCTTTTGATTCTTCGAGAACAAATTGCTCCATTTCACACTGAATTCACCA TTAAGGAAATTTCCCTGGACCTCAAGAAAACTAGAGATGCAGCATTTAAAATCCTGAACCCTATGACTGT TCCACGATTTTTTAGGCTGAATAGCAACAATGCCTTGATAGAGTTCTTGTTGGAGGGGACTCCTGAGATC AGAGAACATTATCTTGACTCTAAGAAGGACGTAGACCGGCACCTGAAGTCAGCCTGTGAGCAGTTCATTC AGCAGCAGACCAGGCTGTTTGGAGAGCAGCTGGAGGAGTTCATGACAAAGGTATCAGCATTGAAAACCAT GGCCAGCCAGGGAGGCCCCAAGTACACCCTCTCACAGCAGCCGTGGGCACAGCCAGCAAAGGTCAATGAC CTCGTGGCCACTGCCTACAAGACAATAAAGACGAAGCTGCCGCTGACACTGAGAAGCATGGCTTTGTATT TGTCCAATAAGGACACGGAGTTCATCTTGTTTAAGCCTGTCAGGAATAACATTCAGCAAGTCTTCCAGAA GTTCCACGCTCTGCTAAAGGAAGAGTTCAGTTCTGAAGACATCCAAATCATTGCTTGTCCTTCCATGGAA CAGCTGAACCTCCTGCTGTCAGTTTCAAAGTAGTCCCAGTGGACCACGAGTGTCAGCAGTCTGTCCCACA GGAGAGGCCTGAGGAGTCCTGCAGCCTGCAGGATGCCCAAGACCCCCATGTGGGACGGCTCCTGTAGAAC ATGGATGTGCAGCCTGCAGAAGGAAACCTGGGTGAGCCAGAGAAGATCAAAATGCCAAAGACTGACAGCA GGGATAGCTGCCTCATTGAACTGGTCTCCAGCAGTGGAGAGAAGCGACAGGGGGCCACGATTCACAGTGA AATGTTCCTTCACACAGCTTGTGCAATAAAGTAGGTTACAGTAGTGACTAGTGTGATGCACTCATAGGAA CTCAGCTGACTGGAAAGCTGCCGGGTCTCAGCAAGGCATTCACCCCGGCCATTCCTCGGACCCTGTGACT TGGAGAACTCTTCCTTCTGTGTCCTGATGCAGAGCTTCTCATGCTGGGTCGAGCGAGGTTGAGTTGGAAT CGGATGGTTGCTGCCAAGAAGGCATCTAGCTTTTCCTGGACTCAGGCTGTCATCTTTCTGTGGGCTGACA CAAGGCAGGGGCAGTTGCAAGAAGCCAAGGCCTCCTATAAGGCTCCTTGAACTGCATTCTCCTCACTAGA GAGGTGTATCTTGATGATGGGGATCGCCACAGTGCTGTCACCAGTGTGTGAGTGGGAGTGACCAGTGAAG AGGTTTTAGCGTGGCACAGCCTGGAGAGGAGGCGGCCTGTGGGGAGTGGGCTCATGGATGGAGCTTAGTT GCAGTCATTCTTTATAAAGTGAATACTCTTTGGTTGCCTATGTTGTCCTAGACACTAGCCGTCCGGCATC CCCAGTCCTGCCATAGCTGAACACCTAAGTGTGGCAAACGCTACCCGTACCTTAAATATGACACCAGTCA AAAGTCAAAGGACTATAAGCACCTGTGAATTTCCCTACAAATTCCCCTTTGAATTTGGGATTGGATTGGC TTTGACCCTGGTGTTACAATAAATGTAGCCAGTTTCTTGGTTCAGTTACTCAGCCCGTCGTCTGTAGGGT GGGACAGTGCTGAGGGCTCTGAAATAGGGCAGATGACCTCATGGTGCAGGTGAAGGGACAGGAACAGCCT CTTGCACAGTTGCACGTGGGTGACCCATCCCGATGCAACAGGCCAGTTTCTCAGCCCAGTTTAAATTGGT GGTGGCCGTTGCTTGGTAGAGCATATTACTCTGTATTTTGGGAAACTCAGAGGTAAGAGGGTCCATATTT TTTTTTTGCTATGAAATGAACGAGTTAGGAAGATTGTACTTACTATTTTAAATACATGTCTTATGTGATA AACCGTAATCTCCAGTTCTGTGTATTATATGTACATTGGGTGTTCAGATAGCCTTTCCAAAGAAGCGAGG ACGTGACCGTTGTACAGTGCTGTGGCTCAGCAGTCTGATGGACAGAGCAGGACTTGTTCGAGGACTCTTG TAGTGCTTTGATTTGTTTCTGAAAACACTCACATACCTTACCAACTGCTGGGAGGGTGGGACTGATCCAG TTTGTTTGCTGAATGAATATTTGTTTAAATCTGTACAGTTTCACGTGTTTACTTATACAGAGTGTACATA CTTTGAAATAATTAATTTAAAATGCTTTATATTATTTAGCTAGAAATTGCTGTTTTTAATAAATAAATTT TTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4020725 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4020725 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12810 same embryo
	EMAGE:12808 same embryo
	EurExpress:euxassay_013611 same experiment
	MGI:4823971 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source