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EMAGE:12817

St3gal2 ST3 beta-galactoside alpha-2,3-sialyltransferase 2 ( MGI:99427)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12817 EMAGE:12817 EMAGE:12817 EMAGE:12817 EMAGE:12817
"Pseudo-wholemount" of euxassay_013602. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013602_01 euxassay_013602_02 euxassay_013602_03 euxassay_013602_04
EMAGE:12817 EMAGE:12817 EMAGE:12817 EMAGE:12817 EMAGE:12817
euxassay_013602_05 euxassay_013602_06 euxassay_013602_07 euxassay_013602_08 euxassay_013602_09
EMAGE:12817 EMAGE:12817 EMAGE:12817 EMAGE:12817 EMAGE:12817
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euxassay_013602_15 euxassay_013602_16 euxassay_013602_17 euxassay_013602_18 euxassay_013602_19
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euxassay_013602_20 euxassay_013602_21 euxassay_013602_22 euxassay_013602_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12817Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12817_wholemount_strong.wlz
12817_wholemount_moderate.wlz
12817_wholemount_weak.wlz
12817_wholemount_possible.wlz
12817_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12817_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31990
Entity Detected:St3gal2, ST3 beta-galactoside alpha-2,3-sialyltransferase 2 ( MGI:99427)
Sequence:sense strand is shown

>T31990
GCCTGCCAATGTCAGCTTCGTGCTGGTCCCCTTCAAGGCCCTGGACCTAATGTGGATTGCCAGCGCCCTC
TCCACAGGACAGATCCGATTCACCTATGCCCCAGTGAAGTCCTTCCTTCGAGTGGACAAAGAGAAGGTCC
AGATTTACAACCCAGCCTTCTTCAAGTACATTCACGACAGGTGGACCGAGCACCACGGGCGCTACCCTTC
CACAGGAATGCTGGTGCTCTTCTTTGCACTACATGTGTGTGATGAGGTGAACGTGTATGGGTTCGGGGCC
GACAGTCGAGGCAACTGGCACCACTACTGGGAGAACAACCGGTACGCGGGCGAGTTCCGGAAGACAGGCG
TGCATGACGCCGACTTCGAAGCCCACATCATCGACATGCTGGCCAAGGCCAGCAAGATCGAGGTCTACCG
AGGGAACTGAGCGGCCCTGAGCGCCTTCAGAACCAGTCACTTCTGGGTCCGGCAAGCGCCGGTTGCAGCC
CACCAATCAGACTGCGCCGTCCCCAGCAACCAATCAGTGCTGCAGTTGGGAGGAGCTTCTTTCTTAGCCA
ATCACGCAACTCAAGGAGAATTTCCGGCGCCGCCGTCTCCACCAATCATGGCCTTGGGAGGCGGCGGGCC
TCGACCCCCTCCCCTAAACCTGCTTTGGATAAGAATTTTGATGCTTTTTAAGAGGCGGGTCTGGGGCTGG
GCAGGAGCGCCTTGAACTGCTCATTTCCAGCTTAGGCCGGGTGGGTGAGTGAAGCCATCCAAAGAAATGT
GGTTCCGGTGAGACACTCTCCAGGGCTTTCCGTGGCGCACCAGGTAGTAGGGGGTTGAATTCACCCCCTG
TTGGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4211307), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17725. Forward Primer - name:017725_F_IRAV28-31_K17_Siat5, sequence:GCCTGCCAATGTCAGCTT; Reverse Primer - name:017725_R_SP6_IRAV28-31_K17_Siat5, sequence:ACCCAACAGGGGGTGAAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12816 same embryo
 EMAGE:12819 same embryo
 EMAGE:12815 same embryo
 EMAGE:12818 same embryo
 EMAGE:12814 same embryo
 EurExpress:euxassay_013602 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS