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EMAGE:12819

Vrk2 vaccinia related kinase 2 ( MGI:1917172)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12819 EMAGE:12819 EMAGE:12819 EMAGE:12819 EMAGE:12819
"Pseudo-wholemount" of euxassay_011671. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011671_01 euxassay_011671_02 euxassay_011671_03 euxassay_011671_04
EMAGE:12819 EMAGE:12819 EMAGE:12819 EMAGE:12819 EMAGE:12819
euxassay_011671_05 euxassay_011671_06 euxassay_011671_07 euxassay_011671_08 euxassay_011671_09
EMAGE:12819 EMAGE:12819 EMAGE:12819 EMAGE:12819 EMAGE:12819
euxassay_011671_10 euxassay_011671_11 euxassay_011671_12 euxassay_011671_13 euxassay_011671_14
EMAGE:12819 EMAGE:12819 EMAGE:12819 EMAGE:12819 EMAGE:12819
euxassay_011671_15 euxassay_011671_16 euxassay_011671_17 euxassay_011671_18 euxassay_011671_19
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euxassay_011671_20 euxassay_011671_21 euxassay_011671_22 euxassay_011671_23 euxassay_011671_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12819Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12819_wholemount_strong.wlz
12819_wholemount_moderate.wlz
12819_wholemount_weak.wlz
12819_wholemount_possible.wlz
12819_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12819_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
cerebral cortex marginal layer
moderate moderate
single cellmoderate expression: see section 05 06 07 19 weak expression: see section 03 04 08 09 10 14 15 16 17 18 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31985
Entity Detected:Vrk2, vaccinia related kinase 2 ( MGI:1917172)
Sequence:sense strand is shown

>T31985
GATCGGCTCTGGAGGGTTTGGACTGATATACTTAGCTTTCCCCACAAATAAACCAAACAAAGATGCAAGA
CATGTCATAAAGCTGGAGTATCAAGAAAATGGCCCATTATTTTCAGAGCTTAAATTTTATCAGAGAGCTG
CAAAAAGAGAATGTATCCAAAAGTGGATACAACAGAGGAAACTTGATTATTTAGGAATTCCTGTATTTTA
TGGATTTGGTCTGACTGATTTCAAAGGAAGAAGTTACAGATTCATGGTAATGGAAAGACTTGGAATAGAT
TTACAGAAACTCCTGGACCAGAATGGTGGTTTTAAAAAATTAACCGTCCTACAACTTGGCATCAGGATGC
TGGATGTACTGGAATATATACATGAAAATGAGTATGTTCATGGTGATATAAAAGCCGCAAATCTACTGTT
GGATTTTACAAATCCAGACCGGGTTTATCTTGCAGACTATGGACTTTCCTACAGATATTGTCCCAATGGG
AACCACAAACAGTATCAGGAAGATCCCAGAAAGGGCCATAATGGGACAATAGAGTTTACAAGTTTGGATG
CACACAAAGGAGTGGCCCCATCCAGGAGGAGTGATGTTGAAATCCTTGGTTACTGCATGCTGCACTGGCT
CTTCGGGAAGCTTCCTTGGGAAGCAAAGCTGGACGACCCTGTGGCTGTCCAGACTGCTAAAACCAACCTG
CTGGATGAACTCCCAGAGTCGGTGCTTAAGTGGGCTCCTTCTGGAAGCAGTTGCAGTGAACTTGTCAAGT
ACTTGATGTATGTTCATAATTTAGCTTATGATGACAAGCCAGACTATCAGAAGCTCAAGAAAATTTTGAA
TCCAGATGGAGTACTTTTAGGGCCACTGGAATTTTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4236402), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 19391. Forward Primer - name:019391_F_IRAV28-31_D23_Vrk2, sequence:GATCGGCTCTGGAGGGTT; Reverse Primer - name:019391_R_SP6_IRAV28-31_D23_Vrk2, sequence:GGAAAATTCCAGTGGCCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12816 same embryo
 EMAGE:12815 same embryo
 EMAGE:12818 same embryo
 EMAGE:12817 same embryo
 EMAGE:12814 same embryo
 EurExpress:euxassay_011671 same experiment
 MGI:4829175 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS