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EMAGE:1283

Otx2 orthodenticle homolog 2 (Drosophila) ( MGI:97451)
TS08 (6.5 dpc)
immunohistochemistry

Data Images
EMAGE:1283
Fig 3A' Boyl et al, 2001 [PMID:11532921] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who own the copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
This section is described as sagittal by the authors. Image annotations: epi - epiblast, AVE - anterior visceral endoderm.
Expression Pattern Description
Spatial Annotation:
EMAGE:1283EMAGE:1283Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
1283_voxel_strong_3D_1.wlz
1283_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1283_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
epiblast
moderate moderate
primitive endoderm
moderate moderate
regionalExpression in Anterior (Embryonic) Visceral Endoderm
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1309322
Entity Detected:Otx2, orthodenticle homolog 2 (Drosophila) ( MGI:97451)
Notes:The anti-OTX2 antisera used in this study by Boyl et al, 2001 [PMID:11532921] is indicated as that used by Acampora et al, 1998 [PMID:9811592] " i.e.: "polyclonal antisera directed against OTX2 (Mallamaci et al., 1996 [PMID:8887325] ). Mallamaci et al describe the Otx2 antigen as being translated from a "0.9kb filled-in EcoRI-EcoRI fragment, corresponding to the coding region of the murine Otx2 cDNA (Simeone et al, 1993 [PMID:8101484] )". The sequence is not reported Simeone et al - exact end points of antigen sequence undefined.
Antibody Type:polyclonal
Raised In:rabbit
Specimen
Organism:mouse
Age:6.5 dpc
Theiler Stage:TS08
Mutations:none (wild-type)
Preparation:section
Procedures
General Information
Authors:Boyl et al, 2001 [PMID:11532921] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11532921] Boyl PP, Signore M, Acampora D, Martinez-Barbera JP, Ilengo C, Annino A, Corte G, Simeone A 2001 Forebrain and midbrain development requires epiblast-restricted Otx2 translational control mediated by its 3' UTR. Development (128):2989-3000
 [ PMID:9811592] Acampora D, Avantaggiato V, Tuorto F, Briata P, Corte G, Simeone A 1998 Visceral endoderm-restricted translation of Otx1 mediates recovery of Otx2 requirements for specification of anterior neural plate and normal gastrulation. Development (125):5091-104
 [ doi:10.1016/S0925-4773(96)00571-0] [ PMID:8887325] Mallamaci A, Di Blas E, Briata P, Boncinelli E, Corte G 1996 OTX2 homeoprotein in the developing central nervous system and migratory cells of the olfactory area. Mech Dev (58):165-78
 [ PMID:8101484] Simeone A, Acampora D, Mallamaci A, Stornaiuolo A, D'Apice MR, Nigro V, Boncinelli E 1993 A vertebrate gene related to orthodenticle contains a homeodomain of the bicoid class and demarcates anterior neuroectoderm in the gastrulating mouse embryo. EMBO J (12):2735-47
Links:MGI:2150149 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI