EMAGE mouse gene expression spatial database

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EMAGE:12834

Zfr zinc finger RNA binding protein ( MGI:1341890)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011649. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011649_01	euxassay_011649_02	euxassay_011649_03	euxassay_011649_04

euxassay_011649_05	euxassay_011649_06	euxassay_011649_07	euxassay_011649_08	euxassay_011649_09

euxassay_011649_10	euxassay_011649_11	euxassay_011649_12	euxassay_011649_13	euxassay_011649_14

euxassay_011649_15	euxassay_011649_16	euxassay_011649_17	euxassay_011649_18	euxassay_011649_19

euxassay_011649_20	euxassay_011649_21	euxassay_011649_22	euxassay_011649_23	euxassay_011649_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12834_wholemount_strong.wlz

12834_wholemount_moderate.wlz

12834_wholemount_weak.wlz

12834_wholemount_possible.wlz

12834_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12834_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 06 07 08 09 17 18 19 20

vibrissa

weak

regional

weak expression: see section 05 06 07 19 20 21

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 07 08 09 16

pons mantle layer

weak

regional

weak expression: see section 05 16

facial vii ganglion

weak

regional

weak expression: see section 03 19 20 21

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 05 06 18 19

trigeminal v ganglion

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 16 17 18 19 20 21 22

vagus x ganglion

weak

regional

weak expression: see section 07 17

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 05 06 07 18 19

trigeminal v nerve

weak

regional

weak expression: see section 09 16

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 09 10 11 12 16 17 18 19 weak expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31667
Entity Detected:	Zfr, zinc finger RNA binding protein ( MGI:1341890)
Sequence:	sense strand is shown >T31667 AGGCAGCCCTGGACTTCTGGACCCTTGTGAAAAGGATCCCTTTGACACTTTGGCAACAATGACTGACCAA CAGCGTGAAGACATTACATCCAGTGCACAGTTTGCATTGAGACTCCTTGCATTCCGTCAGATACACAAAG TTCTGGGGATGGATCCATTACCACAAATGAATCAGCGCTTTAACATCCACAACAACAGGAAACGAAGAAG GGATAGTGATGGAGTGGATGGATTTGAAGCTGAGGGGAAGAAAGACAAAAAGGATTATGATAACTTTTAA AAAGTTTGTGTAAATCTTCAGTGTTACAGTAGGTGCCGGTTTATTGGCTGTTTTCCATTCATATTAATCT CTACTTTAAAAGTTTATCAAGAGTATAAAGAGTTTCATGGAAGAACCAAGTTTTTCTATGATTAAAAACT TATTGTACAGTGTCAGGTATTATATGGATGGAAAGAAAGAGATTTGACTAGGGGGAATATATTGGTCTTT CCCCCCCCCCACCCTTTTTCAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3154019), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 61579. Forward Primer - name:061579_F_IRAV08-11_P21_Zfr, sequence:AGGCAGCCCTGGACTTCT; Reverse Primer - name:061579_R_SP6_IRAV08-11_P21_Zfr, sequence:AATGAAAAAGGGTGGGGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12833 same embryo
	EMAGE:12837 same embryo
	EMAGE:12835 same embryo
	EMAGE:12836 same embryo
	EurExpress:euxassay_011649 same experiment
	MGI:4829348 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source