EMAGE mouse gene expression spatial database

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EMAGE:12835

Npepps aminopeptidase puromycin sensitive ( MGI:1101358)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011604. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011604_01	euxassay_011604_02	euxassay_011604_03	euxassay_011604_04

euxassay_011604_05	euxassay_011604_06	euxassay_011604_07	euxassay_011604_08	euxassay_011604_09

euxassay_011604_10	euxassay_011604_11	euxassay_011604_12	euxassay_011604_13	euxassay_011604_14

euxassay_011604_15	euxassay_011604_16	euxassay_011604_17	euxassay_011604_18	euxassay_011604_19

euxassay_011604_20	euxassay_011604_21	euxassay_011604_22	euxassay_011604_23	euxassay_011604_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12835_wholemount_strong.wlz

12835_wholemount_moderate.wlz

12835_wholemount_weak.wlz

12835_wholemount_possible.wlz

12835_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12835_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 06 07 08 09 17 18 19 20

vibrissa

weak

regional

weak expression: see section 05 06 21

facial vii ganglion

weak

regional

weak expression: see section 03 04 05 20 21

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07 18

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 07 08 17 18 19 20 21 22

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 05 06 07 17 18 19

dorsal root ganglion

moderate

regional

moderate expression: see section 07 09 10 11 12 17 18 19

neural retina

moderate

regional

moderate expression: see section 01 02 22 23 24

midgut

moderate

regional

moderate expression: see section 09 10 11 17 18 19 weak expression: see section 07 08 12 13 14 15 16

bladder

weak

regional

Expression in the fundus region.

urethra of male

weak

regional

weak expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31666
Entity Detected:	Npepps, aminopeptidase puromycin sensitive ( MGI:1101358)
Sequence:	sense strand is shown >T31666 TTATACCCGTGCCCAGGAACTTGATGCCTTAGATAACAGCCATCCTATTGAAGTCAGTGTGGGCCATCCG TCTGAGGTTGATGAGATATTTGATGCTATATCATATAGCAAAGGTGCATCTGTAATCCGAATGCTACATG ACTACATTGGTGATAAGGACTTTAAGAAAGGAATGAATATGTATTTAACCAAGTTCCAACAAAAGAATGC TGCCACAGAGGATCTCTGGGAAAGTTTGGAAAGTGCCAGTGGCAAACCCATAGCAGCTGTGATGAATACC TGGACCAAACAAATGGGATTCCCTCTCATTTATGTGGAAGCTGAACAGGTAGAAGATGACAGAGTGCTGA AGCTGTCTCAGAAGAAGTTTTGTGCCAGTGGACCATATGGCGGTGAAGACTGTCCTCAGTGGATGGTTCC TATCACAATTTCAACTAGTGAGGATCCTAACCAGGCTAAGCTGAAAATACTAATGGATAAGCCAGAGATG AGTGTGGTTTTGAAAAATGTCAAACCAGACCAATGGGTAAAGCTAAATCTGGGAACAGTTGGGTTTTATC GAACCCAGTACAGCTCTGCCATGCTCGAAAGTTTATTACCAGGCATCCGTGACCTTTCTCTGCCCCCAGT GGATCGACTTGGATTACAGAATGACCTCTTTTCTCTGGCTCGAGCTGGCATCATTAGCACTGTAGAGGTT CTAAAAGTCATGGAGGCTTTTGTGAATGAGCCCAATTATACTGTATGGAGCGACCTGAGCTGTAACCTGG GGATTCTTTCAACTCTCTTGTCCCACACAGACTTCTATGAGGAAATCCAGGAGTTTGTCAAAGATGTCTT TTCACCTATAGGAGAGAGATTGGGCTGGGAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3487224), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 10911. Forward Primer - name:010911_F_IRAV08-11_P20_Npepps, sequence:TTATACCCGTGCCCAGGA; Reverse Primer - name:010911_R_SP6_IRAV08-11_P20_Npepps, sequence:GGTCCCAGCCCAATCTCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12833 same embryo
	EMAGE:12837 same embryo
	EMAGE:12836 same embryo
	EMAGE:12834 same embryo
	EurExpress:euxassay_011604 same experiment
	MGI:4826762 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source