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EMAGE:12852

Arl6ip6 ADP-ribosylation factor-like 6 interacting protein 6 ( MGI:1929507)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12852 EMAGE:12852 EMAGE:12852 EMAGE:12852 EMAGE:12852
"Pseudo-wholemount" of euxassay_011620. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011620_01 euxassay_011620_02 euxassay_011620_03 euxassay_011620_04
EMAGE:12852 EMAGE:12852 EMAGE:12852 EMAGE:12852 EMAGE:12852
euxassay_011620_05 euxassay_011620_06 euxassay_011620_07 euxassay_011620_08 euxassay_011620_09
EMAGE:12852 EMAGE:12852 EMAGE:12852 EMAGE:12852 EMAGE:12852
euxassay_011620_10 euxassay_011620_11 euxassay_011620_12 euxassay_011620_13 euxassay_011620_14
EMAGE:12852 EMAGE:12852 EMAGE:12852 EMAGE:12852 EMAGE:12852
euxassay_011620_15 euxassay_011620_16 euxassay_011620_17 euxassay_011620_18 euxassay_011620_19
EMAGE:12852 EMAGE:12852 EMAGE:12852
euxassay_011620_20 euxassay_011620_21 euxassay_011620_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12852Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12852_wholemount_strong.wlz
12852_wholemount_moderate.wlz
12852_wholemount_weak.wlz
12852_wholemount_possible.wlz
12852_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12852_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
weak weak
regionalweak expression: see section 09 10 11 18 19 20 21
olfactory cortex ventricular layer
moderate moderate
homogeneousmoderate expression: see section 12 13 17 18 19
telencephalon ventricular layer
moderate moderate
homogeneousmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 weak expression: see section 01 02
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 13 14 15 weak expression: see section 09 10 11 12 16
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 10 11 12 13 14 16 17 18 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31007
Entity Detected:Arl6ip6, ADP-ribosylation factor-like 6 interacting protein 6 ( MGI:1929507)
Sequence:sense strand is shown

>T31007
TCGTCGGAGACCAAGAGGGCCCCGCTACTCCCCCGGGTCGGGGACGGGTCTCCGGTCCTGCCGGATAAGC
GCAACGGCATCTTCCCGGCCACTGCGGCCAAGCGAACCCAGGCTCGGCGGTGGCCCATCCAAGCCCTTTC
CATTCTCTGTTCGCTGCTGTTCGCCGTCCTCCTCGCTTTCCTTCTCGCCATCGCCTACATGATCGTTAAA
GAATTGCATGCAGAAAACTTGAAAAATGAAGATGATATACATACTGGGCTGTTAGGCTTCTGGTCTCTGC
TGATAATATCTCTAACTGCTGGATTGTCCTGTTGCAGCTTTTCTTGGACAGTGACTTACTTTGATTCTTT
TGAACCAGGAATGTTTCCACCCACTCCTCTTTCACCTGCTAGGTTCAAGAAACTGACTGGACATTCTTTC
CACATGGGCTACAGCATGGCAATTTTGAATGGTGTTGTCGCTGCTCTCACTGTGGCATGGTGCCTCATGT
AAACCTACACTAGAGCAACGTTGTTGGTGAAAGTCATGATGGTTTTTGAAGTAGCAGAAACGAAATTGCT
GACTCAGAAGACTTTGAAATGTGATCAGATACTTACTATCATCATTATGGAAGACCTTATAGAGGGCACT
TCTTCAATGTATAAGATGTTTACTTCATCTTTTTACTTTTGTTTATGTAGTTTATCAGTTACAGAAAATA
TTTGAATTGAAATCAAACTTGGAAACTTGAATACATGACATTGCCTACCTTTCTATGTATATTCCATTTT
TTACTAACAGATACAGATATTTGTGGTTGAGGTAGAAATATTTATATACTATATTAGGAAAACAGTACTG
AGGCCAAGTGTGTTAGCAAGACCTTCAAGCCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4210432), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22911. Forward Primer - name:022911_F_IRAV32-35_J15_Arl6ip6, sequence:TCGTCGGAGACCAAGAGG; Reverse Primer - name:022911_R_SP6_IRAV32-35_J15_Arl6ip6, sequence:TGGGCTTGAAGGTCTTGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12853 same embryo
 EMAGE:12851 same embryo
 EMAGE:12849 same embryo
 EMAGE:12850 same embryo
 EurExpress:euxassay_011620 same experiment
 MGI:4823243 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS