Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12869

Psmb8 proteasome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7) ( MGI:1346527)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12869 EMAGE:12869 EMAGE:12869 EMAGE:12869 EMAGE:12869
"Pseudo-wholemount" of euxassay_013665. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013665_01 euxassay_013665_02 euxassay_013665_03 euxassay_013665_04
EMAGE:12869 EMAGE:12869 EMAGE:12869 EMAGE:12869 EMAGE:12869
euxassay_013665_05 euxassay_013665_06 euxassay_013665_07 euxassay_013665_08 euxassay_013665_09
EMAGE:12869 EMAGE:12869 EMAGE:12869 EMAGE:12869 EMAGE:12869
euxassay_013665_10 euxassay_013665_11 euxassay_013665_12 euxassay_013665_13 euxassay_013665_14
EMAGE:12869 EMAGE:12869 EMAGE:12869 EMAGE:12869 EMAGE:12869
euxassay_013665_15 euxassay_013665_16 euxassay_013665_17 euxassay_013665_18 euxassay_013665_19
EMAGE:12869 EMAGE:12869 EMAGE:12869 EMAGE:12869 EMAGE:12869
euxassay_013665_20 euxassay_013665_21 euxassay_013665_22 euxassay_013665_23 euxassay_013665_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12869Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12869_wholemount_strong.wlz
12869_wholemount_moderate.wlz
12869_wholemount_weak.wlz
12869_wholemount_possible.wlz
12869_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12869_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
strong strong
regionalstrong expression: see section 11 12 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31637
Entity Detected:Psmb8, proteasome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7) ( MGI:1346527)
Sequence:sense strand is shown

>T31637
ACAGTTTCTCCGCGCAAGCTCCGGAGCTCGCACTTCCCCGGGGAATGCAGCCCACCGCATTCCTGAGGTC
CTTTGGTGGTGACCAGGAAAGGAATGTTCAAATTGAGATGGCCCACGGCACAACCACACTCGCCTTCAAG
TTCCAGCATGGCGTCATCGTGGCTGTGGACTCCAGGGCCACTGCAGGGAGTTACATTAGCTCCTTAAGGA
TGAACAAAGTGATCGAGATTAACCCTTACCTGCTTGGCACCATGTCTGGTTGTGCAGCCGACTGCCAGTA
CTGGGAGAGGCTGTTGGCCAAGGAGTGCAGGTTGTATTATCTTCGGAATGGGGAACGCATCTCCGTGTCT
GCAGCATCCAAGCTGCTTTCCAACATGATGCTGCAGTACCGGGGGATGGGCCTCTCCATGGGCAGCATGA
TCTGTGGCTGGGACAAGAAGGGACCAGGACTTTACTACGTAGATGACAATGGGACTCGGCTCTCGGGACA
GATGTTTTCCACTGGCAGCGGGAACACCTATGCCTACGGGGTGATGGACAGTGGTTACCGGCAGGACCTC
AGTCCTGAAGAGGCCTACGACCTTGGCCGCAGAGCTATTGCTTATGCTACCCACAGAGACAACTATTCTG
GAGGAGTCGTCAACATGTACCATATGAAGGAAGACGGTTGGGTGAAAGTGGAGAGTTCCGATGTCAGTGA
CCTGCTGTACAAGTACCGAGAGGCCGCTCTGTGATGGCTGCTGGGCAGGCCTCCCCCAGCATTGGTGGCA
CTGGCTGGCAGACTCAGAGACCTGGGACTACTTCAGTCTTAGGAAAAAGAAGGGCTCAACCTGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:2654130), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 12384. Forward Primer - name:012384_F_IRAV08-11_F08_Psmb8, sequence:ACAGTTTCTCCGCGCAAG; Reverse Primer - name:012384_R_SP6_IRAV08-11_F08_Psmb8, sequence:CCCAGGTTGAGCCCTTCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12870 same embryo
 EurExpress:euxassay_013665 same experiment
 MGI:4827479 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS