EMAGE mouse gene expression spatial database

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EMAGE:12878

Dock7 dedicator of cytokinesis 7 ( MGI:1914549)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013589. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013589_01	euxassay_013589_02	euxassay_013589_03	euxassay_013589_04

euxassay_013589_05	euxassay_013589_06	euxassay_013589_07	euxassay_013589_08	euxassay_013589_09

euxassay_013589_10	euxassay_013589_11	euxassay_013589_12	euxassay_013589_13	euxassay_013589_14

euxassay_013589_15	euxassay_013589_16	euxassay_013589_17	euxassay_013589_18	euxassay_013589_19

euxassay_013589_20	euxassay_013589_21	euxassay_013589_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12878_wholemount_strong.wlz

12878_wholemount_moderate.wlz

12878_wholemount_weak.wlz

12878_wholemount_possible.wlz

12878_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12878_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 08 09

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 07 08

telencephalon mantle layer

weak

regional

weak expression: see section 06 07

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 05 06 07

metencephalon rest of alar plate mantle layer

weak

regional

weak expression: see section 07

pharyngo-tympanic tube

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08

cornea epithelium

moderate

regional

moderate expression: see section 05

stomach

weak

regional

weak expression: see section 03 04 05 06 07 08

lower jaw molar

moderate

regional

moderate expression: see section 08

oral epithelium

moderate

regional

moderate expression: see section 07 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31715
Entity Detected:	Dock7, dedicator of cytokinesis 7 ( MGI:1914549)
Sequence:	sense strand is shown >T31715 CCATCGCCTTAAAGAGGCTCTGCAGCCACTGATAAACAGAAAAATCCCTCAGCTATACAAGGCTGTGTTG CCTGTCACTTGCCACAGAGATTCCTTCAGCCGAATGAGCCTTCGAAAAATGGAACTCTAAAATACACACT TGTTCTATTCATTTGAAAAGAACCATGTATTCAACACTGAGTGTGAGGTCTATTGAAAAATAGGACTTGG ATGTCATTCTGGAAGCAATAGTATCGATTTACTCTCTGAAGAATGTGCTCCCAGATGCAGGCTGTTACGT GTGAGGCTTGTGGCGTGGTTTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5355798), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60711. Forward Primer - name:060711_F_IRAV87_h10_Dock7, sequence:CCATCGCCTTAAAGAGGCT; Reverse Primer - name:060711_R_SP6_IRAV87_h10_Dock7, sequence:AGAAACCACGCCACAAGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12880 same embryo
	EMAGE:12882 same embryo
	EMAGE:12879 same embryo
	EMAGE:12881 same embryo
	EMAGE:12877 same embryo
	EurExpress:euxassay_013589 same experiment
	MGI:4824371 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source