EMAGE mouse gene expression spatial database

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EMAGE:12900

Itih5 inter-alpha (globulin) inhibitor H5 ( MGI:1925751)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013596. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013596_01	euxassay_013596_02	euxassay_013596_03	euxassay_013596_04

euxassay_013596_05	euxassay_013596_06	euxassay_013596_07	euxassay_013596_08	euxassay_013596_09

euxassay_013596_10	euxassay_013596_11	euxassay_013596_12	euxassay_013596_13	euxassay_013596_14

euxassay_013596_15	euxassay_013596_16	euxassay_013596_17	euxassay_013596_18	euxassay_013596_19

euxassay_013596_20	euxassay_013596_21	euxassay_013596_22	euxassay_013596_23	euxassay_013596_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12900_wholemount_strong.wlz

12900_wholemount_moderate.wlz

12900_wholemount_weak.wlz

12900_wholemount_possible.wlz

12900_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12900_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 15 16 17 18 19 weak expression: see section 03 04 07 08 12 13 14 20 21 22 23 24

forearm rest of mesenchyme

moderate

regional

moderate expression: see section 01 02

upper arm rest of mesenchyme

moderate

regional

moderate expression: see section 01 02 03

interdigital region between forelimb digits 1 and 2

moderate

regional

moderate expression: see section 04 05

interdigital region between forelimb digits 2 and 3

moderate

regional

moderate expression: see section 05

carpus

moderate

regional

moderate expression: see section 01 02 03 04

interdigital region between hindlimb digits 1 and 2

moderate

regional

moderate expression: see section 05 24

interdigital region between hindlimb digits 2 and 3

moderate

regional

moderate expression: see section 04 05 24

interdigital region between hindlimb digits 3 and 4

moderate

regional

moderate expression: see section 04 05 06 23 24

interdigital region between hindlimb digits 4 and 5

moderate

regional

moderate expression: see section 04 05 06 23 24

foot mesenchyme

moderate

regional

moderate expression: see section 21 22

cranial musculature

moderate

regional

moderate expression: see section 24 weak expression: see section 02 03 04 05 06 07 23

vibrissa

strong

regional

strong expression: see section 06 07 08 09 10 21 22 23 moderate expression: see section 11 19 20

diencephalon meninges

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

telencephalon meninges

moderate

regional

moderate expression: see section 02 03 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 weak expression: see section 04 24

hindbrain meninges

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 weak expression: see section 24

midbrain meninges

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

spinal cord meninges

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20

saccule

strong

regional

strong expression: see section 07 08 09 10 11 19 20 22 moderate expression: see section 06 21 23

extrinsic ocular muscle

weak

regional

weak expression: see section 02 03 04 05 23 24

nasal septum

moderate

regional

moderate expression: see section 12 13 15 16 17 weak expression: see section 11 14

heart valve

moderate

regional

moderate expression: see section 16 weak expression: see section 13 14 15

esophagus

weak

regional

weak expression: see section 15 16

stomach

weak

regional

weak expression: see section 04 05 06 07 08 09

bladder

moderate

regional

moderate expression: see section 15 17 weak expression: see section 14 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30359
Entity Detected:	Itih5, inter-alpha (globulin) inhibitor H5 ( MGI:1925751)
Sequence:	sense strand is shown >T30359 GGCTGCCATCAAACTGCTCAACAACTACGTGGCCCAGAATGACATTGAAGACCGAAGTGTGTCCCTCATC ATCTTCCTCACCGATGGGAAACCCACCTTTGGGGAGACCAATACCCTCAAGATTCTCAGCAACACCAAGG AAGCCACCAGAGGTCAGATCTGCATCTTCACCGTTGGCATTGGCGATGACGTGGACTTCAAACTGTTAGA GAAACTCTCGCTGGAGAACTGCGGTCTCACGAGGCGTGTTCATGAGGAGGACAAGGCGGGCGCACAGCTC ATCGGGTTCTATGATGAGATCCGGACCCCGCTTCTTTCTGACATCCGCATAGATTATCCTCCTGACGTAG TAGAGCATGCCACCAAGACCCTGTTCCCTAACTACTTCAATGGCTCTGAGATAGTGATTGCAGGGAAGAT GGTGGACAAGAAGTTTGATCAACTGCATGTGGAGGTCACTGCCAGCAACAGTAAGAAATTTGTCATCCTA AAAAGAGACATCCCCGTGGAGTTCCGGAAGATGGGGAATGATGTCTCAGTCACACCTGGCTCAGCCAGAG ATGGCGGGAAGGACCTAAACCACATTGAGCGACTTTGGAGCTACCTCACTGTGAAGGAGCTGCTAAGCTC CTGGAGGCAGAGCAACAGTGAGCAAGAGAAAGAGCAGCTGAGGCAGAAGGCCCAGGACTTAGCCTTGAAC TATCATTTCCTCACCCCCTTCACCTCCATGAAGCTGAGGAAGCCAGGGCTCCGCACAAACCAGCTGGAGG ACACCTATGGCATGTCTGCAGCTACAGGACCTGCGACCGTGGTGCAGAACCTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3490706), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60480. Forward Primer - name:060480_F_IRAV100_g05_Itih5, sequence:GGCTGCCATCAAACTGCT; Reverse Primer - name:060480_R_SP6_IRAV100_g05_Itih5, sequence:GAAGGTTCTGCACCACGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12899 same embryo
	EMAGE:12901 same embryo
	EMAGE:12897 same embryo
	EMAGE:12898 same embryo
	EurExpress:euxassay_013596 same experiment
	MGI:4825662 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source