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EMAGE:12911

Dazap1 DAZ associated protein 1 ( MGI:1917498)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12911 EMAGE:12911 EMAGE:12911 EMAGE:12911 EMAGE:12911
"Pseudo-wholemount" of euxassay_011657. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011657_01 euxassay_011657_02 euxassay_011657_03 euxassay_011657_04
EMAGE:12911 EMAGE:12911 EMAGE:12911 EMAGE:12911 EMAGE:12911
euxassay_011657_05 euxassay_011657_06 euxassay_011657_07 euxassay_011657_08 euxassay_011657_09
EMAGE:12911 EMAGE:12911 EMAGE:12911 EMAGE:12911 EMAGE:12911
euxassay_011657_10 euxassay_011657_11 euxassay_011657_12 euxassay_011657_13 euxassay_011657_14
EMAGE:12911 EMAGE:12911 EMAGE:12911 EMAGE:12911 EMAGE:12911
euxassay_011657_15 euxassay_011657_16 euxassay_011657_17 euxassay_011657_18 euxassay_011657_19
EMAGE:12911 EMAGE:12911 EMAGE:12911 EMAGE:12911 EMAGE:12911
euxassay_011657_20 euxassay_011657_21 euxassay_011657_22 euxassay_011657_23 euxassay_011657_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12911Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12911_wholemount_strong.wlz
12911_wholemount_moderate.wlz
12911_wholemount_weak.wlz
12911_wholemount_possible.wlz
12911_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12911_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31956
Entity Detected:Dazap1, DAZ associated protein 1 ( MGI:1917498)
Sequence:sense strand is shown

>T31956
TCGGGAAGCTCTTTGTGGGCGGCCTGGACTGGAGTACCACTCAAGAGACTCTACGCAGCTACTTTTCCCA
GTATGGTGAGGTTGTGGATTGTGTCATCATGAAAGATAAAACCACCAACCAGTCTAGAGGCTTTGGATTT
GTCAAGTTTAAAGACCCCAATTGTGTGGGGACAGTGCTGGCCAGCAGACCACACACCCTAGATGGCCGAA
ATATCGACCCGAAGCCGTGCACACCTCGGGGGATGCAGCCAGAGCGAACACGGCCCAAGGAAGGCTGGGT
AAGGCTGGAAAGGACCCAGGAGCGACAGCAGCAAATCCAACAAGATCTTTGTCGGGGGCATTCCCCACAA
CTGCGGGGAGACGGAGCTCAGGGAGTACTTCAAGAAGTTTGGAGTGGTCACAGAGGTGGTTATGATCTAT
GATGCGGAGAAGCAGCGGCCTCGAGGTTTTGGATTTATTACTTTCGAGGACGAACAATCAGTGGACCAGG
CTGTCAACATGCATTTTCACGACATCATGGGCAAAAAAGTGGAGGTTAAACGGGCTGAACCACGGGACAG
CAAGAATCAAGCTCCAGGACAGCCAGGAGCCAGCCAGTGGGGCAGCCGCGTGGCGCCCAGTGCAGCCAAC
GGCTGGGCAGGCCAGCCCCCGCCCACGTGGCAACAAGGATATGGCCCACAAGGAATGTGGGTGCCAGCAG
GACAGGCCATTGGTGGCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6529568), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59448. Forward Primer - name:059448_F_IRAV100_a03_Dazap1, sequence:TCGGGAAGCTCTTTGTGG; Reverse Primer - name:059448_R_SP6_IRAV100_a03_Dazap1, sequence:TAGCCACCAATGGCCTGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12909 same embryo
 EMAGE:12912 same embryo
 EMAGE:12908 same embryo
 EMAGE:12910 same embryo
 EMAGE:12907 same embryo
 EurExpress:euxassay_011657 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS