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EMAGE:12951

Xkr9 X Kell blood group precursor related family member 9 homolog ( MGI:2686466)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12951 EMAGE:12951 EMAGE:12951 EMAGE:12951 EMAGE:12951
"Pseudo-wholemount" of euxassay_013780. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013780_01 euxassay_013780_02 euxassay_013780_03 euxassay_013780_04
EMAGE:12951 EMAGE:12951 EMAGE:12951 EMAGE:12951 EMAGE:12951
euxassay_013780_05 euxassay_013780_06 euxassay_013780_07 euxassay_013780_08 euxassay_013780_09
EMAGE:12951 EMAGE:12951 EMAGE:12951 EMAGE:12951 EMAGE:12951
euxassay_013780_10 euxassay_013780_11 euxassay_013780_12 euxassay_013780_13 euxassay_013780_14
EMAGE:12951 EMAGE:12951 EMAGE:12951 EMAGE:12951 EMAGE:12951
euxassay_013780_15 euxassay_013780_16 euxassay_013780_17 euxassay_013780_18 euxassay_013780_19
EMAGE:12951 EMAGE:12951 EMAGE:12951 EMAGE:12951 EMAGE:12951
euxassay_013780_20 euxassay_013780_21 euxassay_013780_22 euxassay_013780_23 euxassay_013780_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12951Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12951_wholemount_strong.wlz
12951_wholemount_moderate.wlz
12951_wholemount_weak.wlz
12951_wholemount_possible.wlz
12951_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12951_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
midgut
moderate moderate
regionalmoderate expression: see section 10 11 13 14 15 16 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37860
Entity Detected:Xkr9, X Kell blood group precursor related family member 9 homolog ( MGI:2686466)
Sequence:sense strand is shown

>T37860
AAGGCCTTCTTGTACCACTTGATCTGGCGTAGACATTTTTTTTTAAAGGAAGTTAAAGTTATTCACTTTT
GTTTTAGTGTTCCAATTTCATAATATTTATTTATTTATTTTTCGTACTAGGCACTGAATATAGGAGTGTA
TGAATGTTAGATAAACACTCCATCACTGAACTATATCACCATATTCTTTTCACTAGTTAGACTCAGTGTA
TAAATTACAATTCAATGCTAACCCAAAAGATACACTAGTATCCATTGTGGCATTTTCCCCTATTTTTGTA
TCTGAAAAGGAGTAACTAGGCAATAGCCACAGTCCTTCATAATGAAATATACCAAGTGTAATTTTATGAT
GTCCGTTTTGGGCATTATAATCTATGTAACTGATTTAGTTGCAGACATTGTCCTATCTGTTAGGTACTTC
CATGATGGACAATATGTTCTTGGTGTTTTAACCTTGAGCTTTGTGCTTTGTGGAACACTCATAGTCCATT
GTTTTAGCTACTCATGGTTGAAGGCTGACTTAGAGAAAGCAGGACAAGAAAATGAACGTTATTTTCTTCT
ACTTCATTGCTTGCAAGGAGGAGTTTTCACAAGGTATTGGTTTGCCTTGAGAACGGGTTACCATGTGGTT
TTCAAACACAGCGACAGGAAGAGTAATTTTATGGAGGAGCAAACGGATCCTCACAAAGAAGCAATAGACA
TGGCCACCGACTTGAGCATGCTCAGGCTGTTTGAGACCTACCTGGAAGGCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 75457. Forward Primer - name:075457_F_cDNA_LOC381246, sequence:AAGGCCTTCTTGTACCACTTGA; Reverse Primer - name:075457_N_SP6_cDNA_LOC381246, sequence:CAGCCTTCCAGGTAGGTCTCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12956 same embryo
 EMAGE:12954 same embryo
 EMAGE:12953 same embryo
 EMAGE:12955 same embryo
 EMAGE:12952 same embryo
 EurExpress:euxassay_013780 same experiment
 MGI:4829241 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS