EMAGE mouse gene expression spatial database

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EMAGE:12962

Leprel1 leprecan-like 1 ( MGI:2146663)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013656. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013656_01	euxassay_013656_02	euxassay_013656_03	euxassay_013656_04

euxassay_013656_05	euxassay_013656_06	euxassay_013656_07	euxassay_013656_08	euxassay_013656_09

euxassay_013656_10	euxassay_013656_11	euxassay_013656_12	euxassay_013656_13	euxassay_013656_14

euxassay_013656_15	euxassay_013656_16	euxassay_013656_17	euxassay_013656_18	euxassay_013656_19

euxassay_013656_20	euxassay_013656_21	euxassay_013656_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12962_wholemount_strong.wlz

12962_wholemount_moderate.wlz

12962_wholemount_weak.wlz

12962_wholemount_possible.wlz

12962_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12962_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trunk mesenchyme

weak

regional

weak expression: see section 01 02 03 04 05 06 15 17 18 19 20 21 22

diencephalon roof plate

weak

regional

weak expression: see section 11

telencephalon ventricular layer

weak

regional

weak expression: see section 02 03 04 05 06 07 15 16 17 18 19

spinal cord roof plate

strong

regional

strong expression: see section 11 moderate expression: see section 13 weak expression: see section 12

tongue muscle

weak

regional

weak expression: see section 10 11 12 13 14

mandible

weak

regional

weak expression: see section 02 03 04 06 07 18 20 21

metanephros

moderate

regional

moderate expression: see section 07 18

lung

weak

regional

weak expression: see section 04 05 06 07 08 09 10 13 14 15 16 17 18 19

axial skeleton

moderate

regional

moderate expression: see section 13 14 weak expression: see section 11 12

clavicle

strong

regional

strong expression: see section 08 09 15 moderate expression: see section 07 16 weak expression: see section 17

axial skeleton tail region

moderate

regional

moderate expression: see section 13 14 weak expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37779
Entity Detected:	Leprel1, leprecan-like 1 ( MGI:2146663)
Sequence:	sense strand is shown >T37779 TTCCCTTGCATTACGACTACCTCCAGTTTGCCTACTACAGAGTTGGTGAATATGTGAAAGCCTTGGAGTG CGCCAAAGCCTACCTGATGTTCCATCCAGACAATGAGGATGTCCTGGACAATGTAGACTTCTATGAGAGT CTGCTGGATGACAGCACTGATCCAGCATCCATTGAGGCCCGGGAGGATTTAACAGCATTTGTCAAACGTC ATAAACTTGAAGCTGAACTGATCAAGTTAGCTGCAGAGGGCCTGGGATTTTCATATGCTGAACCGAATTA CTGGATCAGCTATGGAGGACGGCAGGATGAGAACCGAGTCCCTTCAGGAGTGAATATGGATGGGGCAGAA GTTCATGGATTGTCAATGGGAAAGAAGTCACCGCCCAAGATAGGTCGAGACCTAAGAGAAGGTGGCCCTC TGCTCTACGAGAACATCACCTTCGTCTACAACTCTGAGCAGCTGAACGGGACTCAACGGGTTCTTCTCGA TAATGTCCTGTCGCAAGAGCAGTGTCGGGAGCTGCACAGTGTGGCCAATGGAATCATGCTGGTTGGTGAT GGATACCGAGGGAAAACGTCACC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 88711. Forward Primer - name:088711_F_cDNA_Leprel1, sequence:TTCCCTTGCATTACGACTACCT; Reverse Primer - name:088711_N_SP6_cDNA_Leprel1, sequence:GGTGACGTTTTCCCTCGGTAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12964 same embryo
	EMAGE:12963 same embryo
	EMAGE:12965 same embryo
	EurExpress:euxassay_013656 same experiment
	MGI:4825902 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source