EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12965

Zdhhc13 zinc finger, DHHC domain containing 13 ( MGI:1919227)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013713. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013713_01	euxassay_013713_02	euxassay_013713_03	euxassay_013713_04

euxassay_013713_05	euxassay_013713_06	euxassay_013713_07	euxassay_013713_08	euxassay_013713_09

euxassay_013713_10	euxassay_013713_11	euxassay_013713_12	euxassay_013713_13	euxassay_013713_14

euxassay_013713_15	euxassay_013713_16	euxassay_013713_17	euxassay_013713_18	euxassay_013713_19

euxassay_013713_20	euxassay_013713_21	euxassay_013713_22	euxassay_013713_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12965_wholemount_strong.wlz

12965_wholemount_moderate.wlz

12965_wholemount_weak.wlz

12965_wholemount_possible.wlz

12965_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12965_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

strong

regional

strong expression: see section 05 06 21 22

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 14 15 16 17 18

vomeronasal organ

strong

regional

strong expression: see section 11 14

esophagus epithelium

strong

regional

strong expression: see section 12

lower jaw molar

strong

regional

strong expression: see section 06 18 19

upper jaw molar

strong

regional

strong expression: see section 06 19

renal cortex

strong

regional

strong expression: see section 06 07 08 09 10 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31969
Entity Detected:	Zdhhc13, zinc finger, DHHC domain containing 13 ( MGI:1919227)
Sequence:	sense strand is shown >T31969 GCCTGGACATCCGAAATGCTAAGGGAGAAACACCTCTTGATATGGCTCTACAAAGTAAAAACCAGCTCAT TAGTCACATGCTAAGAACAGAGGCCAAGATGAGAGCCAACAAACAGTTTCGGCTTTGGCGATGGCTGCAC AAATGCGAGCTCTTCCTGCTGCTGATACTCTCTATGATCACCCTGTGGGCTGTTGGATACATCCTGGACT TCAATTCAGATTCTTGGCTTTTAAAAGGATGTCTTCTAGTAGCATTGTTTTTTCTGACATCTTTGTTTCC AAGGTTTTTGGTTGGCTATAAGAACCTTGTGTACTTACCGACAGTCTTTCTGCTAAGTTCCATTTTTTGG ATATTTATGACTTGGTTCATCTTATTCTTTCCTGATACAGCAGGTAGCCCTTTGTATTTTGCTTTTATTT TCAGCATCATGGCCTTTCTCTATTTTTTCTACAAGACTTGGGCAACTGATCCAGGCTTCACTAAGGCTTC GGAGGAAGAGAGGAAAGTGAATATCGTCACCCTAGCAGAGACTGGCTCTCTGGACTTCAGAACATTTTGC ACATCATGTCTTATAAGGAAGCCTTTAAGATCACTCCATTGCCACGTGTGCAACTCCTGTGTGGCTCGCT TTGATCAGCACTGCTTTTGGACTGGACGCTGCATAGGTTTTGGCAACCATCATCATTACATCTTCTTCTT GCTTTCCCTTTCCATGGTATGTGACTGGATCATATATGGATCTTTCGTCTACTGGTCAAATCATTGTGCC ACAACATTCAAGGAAGATGGACTATGGACCTACCTCAATCAGATAGTGGCCTGTTCTCCCTGGGTTCTGT ATATCTTCATGCTAGCAGCTTTCCACTTCTCATGGTCAACATTTTTATTAATAAATCAACTCTTTCAGAT TGCATTTCTGGGCCTAACCTCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6311721), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58234. Forward Primer - name:058234_F_IRAV101_a09_Zdhhc13, sequence:GCCTGGACATCCGAAATG; Reverse Primer - name:058234_R_SP6_IRAV101_a09_Zdhhc13, sequence:GGGAGGTTAGGCCCAGAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12964 same embryo
	EMAGE:12962 same embryo
	EMAGE:12963 same embryo
	EurExpress:euxassay_013713 same experiment
	MGI:4829280 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source