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EMAGE:13013

Zbtb7a zinc finger and BTB domain containing 7a ( MGI:1335091)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13013 EMAGE:13013 EMAGE:13013 EMAGE:13013 EMAGE:13013
euxassay_017382_01 euxassay_017382_02 euxassay_017382_03 euxassay_017382_04 euxassay_017382_05
EMAGE:13013 EMAGE:13013 EMAGE:13013 EMAGE:13013 EMAGE:13013
euxassay_017382_06 euxassay_017382_07 euxassay_017382_08 euxassay_017382_09 euxassay_017382_10
EMAGE:13013 EMAGE:13013 EMAGE:13013 EMAGE:13013 EMAGE:13013
euxassay_017382_11 euxassay_017382_12 euxassay_017382_13 euxassay_017382_14 euxassay_017382_15
EMAGE:13013 EMAGE:13013 EMAGE:13013 EMAGE:13013 EMAGE:13013
euxassay_017382_16 euxassay_017382_17 euxassay_017382_18 euxassay_017382_19 euxassay_017382_20
EMAGE:13013 EMAGE:13013 EMAGE:13013 EMAGE:13013
euxassay_017382_21 euxassay_017382_22 euxassay_017382_23 euxassay_017382_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13013Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13013_wholemount_strong.wlz
13013_wholemount_moderate.wlz
13013_wholemount_weak.wlz
13013_wholemount_possible.wlz
13013_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13013_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39410
Entity Detected:Zbtb7a, zinc finger and BTB domain containing 7a ( MGI:1335091)
Sequence:sense strand is shown

>T39410
GTGGCCTGAGAGAGATGAAGATGCCCCGCCCGGAGGGCTCTTCCCACCTCCTACTGCCCCACCGGCCACC
ACACAGAACGGCCACTATGGCCGTGCAGGGGCTGGCACCGGTGAAGAAGAAGCGGCGGCTCTCTCTGAGG
CCGCTCCAGAGCCGGGCGACTCCCCGGGCTTCCTGTCAGGCGCTGCAGAGGGCGAGGATGGGGACGCCGC
TGATGTGGATGGGCTAGCGGCCAGCACGCTGCTACAGCAGATGATGTCATCGGTGGGCCGGGCCGGGGAC
AGTGATGAGGAGTCGCGAACCGACGACAAGGGCGTCATGGACTACTACCTGAAGTACTTCAGTGGAGCCC
ACGAGGGGGATGTGTACCCAGCCTGGTCACAGAAGGGTGAGAAGAAAATCCGGGCCAAGGCCTTCCAGAA
GTGTCCCATCTGCGAGAAGGTGATTCAGGGTGCCGGCAAGCTGCCCCGTCACATCCGCACGCACACGGGC
GAGAAGCCCTACGAGTGTAACATCTGTAAAGTTCGATTCACCAGACAGGACAAGCTGAAGGTGCACATG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74377. Forward Primer - name:074377_F_cDNA_Zbtb7, sequence:GTGGCCTGAGAGAGATGAAGAT; Reverse Primer - name:074377_N_SP6_cDNA_Zbtb7, sequence:CATGTGCACCTTCAGCTTGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13012 same embryo
 EMAGE:13010 same embryo
 EMAGE:13011 same embryo
 EurExpress:euxassay_017382 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS