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EMAGE:13016

Slc5a8 solute carrier family 5 (iodide transporter), member 8 ( MGI:2384916)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13016 EMAGE:13016 EMAGE:13016 EMAGE:13016 EMAGE:13016
"Pseudo-wholemount" of euxassay_019726. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019726_01 euxassay_019726_02 euxassay_019726_03 euxassay_019726_04
EMAGE:13016 EMAGE:13016 EMAGE:13016 EMAGE:13016 EMAGE:13016
euxassay_019726_05 euxassay_019726_06 euxassay_019726_07 euxassay_019726_08 euxassay_019726_09
EMAGE:13016 EMAGE:13016 EMAGE:13016 EMAGE:13016 EMAGE:13016
euxassay_019726_10 euxassay_019726_11 euxassay_019726_12 euxassay_019726_13 euxassay_019726_14
EMAGE:13016 EMAGE:13016 EMAGE:13016 EMAGE:13016 EMAGE:13016
euxassay_019726_15 euxassay_019726_16 euxassay_019726_17 euxassay_019726_18 euxassay_019726_19
EMAGE:13016 EMAGE:13016 EMAGE:13016 EMAGE:13016 EMAGE:13016
euxassay_019726_20 euxassay_019726_21 euxassay_019726_22 euxassay_019726_23 euxassay_019726_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13016Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13016_wholemount_strong.wlz
13016_wholemount_moderate.wlz
13016_wholemount_weak.wlz
13016_wholemount_possible.wlz
13016_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13016_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 15 weak expression: see section 12 16
rectum
strong strong
regionalstrong expression: see section 15 weak expression: see section 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T56049
Entity Detected:Slc5a8, solute carrier family 5 (iodide transporter), member 8 ( MGI:2384916)
Sequence:sense strand is shown

>T56049
TGATAACCCTGCCTTCAACCATGTTGAGCTGAACTTCACAGATCACAGTGGCAAGATCAATGGGACTCGC
TTGTGAAGCATCTCCAGGGCTAGATGACATTGTATAATGTCTGTTTTGTTTTTGTTTTGCTTTTCTTTAA
ACATGTTTTAGGATAGTTGGATCAGATCAGTTTTTTCCTATCATGATTGTTTTTGGAAGATATATTTTAT
TAAAGCCAAATCCCGATTTGGCTCACTTTATACACTGGTTGATTGATGCTATCCTGGAGTTAGGAGTTTC
AACACGAGCTCTGGTTGTGGATGTTTATAACTACATACATGTGTGAAAGGCAAATACAGACATACACACA
TGGTTCTTGATAATCAAAGGCATATTCTTACGTTTGGGCATTACTGAAAACATTTTACATGCCTTTGGTG
CTAGTATATGTATATTCTGGGAACTTTACTAGAATAATAAATCTTGGAAAGAGATTTCTTTCCTCCCCCT
GAATATCCTGTTTCTAGGCAGCAGGGACAGGAGGAGGAGTAGAGCACTTCTGTGGACTTTACTTCTGGAT
CAGCCCATGCTATAACCTTGCCTCTGAGTCTGCCTCTGAATGGGTAAATAGCAGCATCCTGCCTAGGATA
CACTAGGTACCCAACGATCAAGTTCATCTTTAATGCATTCTTGTGCCTGTTTTTCCTCTGCTGAGATTCC
TGACAATTGCCAAGTACAGTGCTTTTCATAGACTATTGACTATAGATTATAGACCATTGACTGTAGACTA
TCAACTATCGACTATAATTTTTAATCTTTATTCCCAACTTCTGTTCTAATAACCTTGCTTTTGTAGAGCA
TGTTACCTGGACTTAACTGTTCCAGCTTCTTGCTTTTTGCCCTGGGTTTAGATATGTCTTGAGACATCCA
ATATAAACTATGTCACCTCTTTCCTTCAGTCCCCAGATCCCATCCTGTGCTAACACATGCTTCTTGTGTA
TTGGGTAACCCAAGAACATTAAATATTTGAAAACGGTGAAGTCCCATG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TGATAACCCTGCCTTCAACC; Reverse Primer - name:unspecified, sequence:CATGGGACTTCACCGTTTTC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:BL6/SV129
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13017 same embryo
 EurExpress:euxassay_019726 same experiment
 MGI:4828257 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS