EMAGE mouse gene expression spatial database

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EMAGE:13024

Brip1 BRCA1 interacting protein C-terminal helicase 1 ( MGI:2442836)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013686. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013686_01	euxassay_013686_02	euxassay_013686_03	euxassay_013686_04

euxassay_013686_05	euxassay_013686_06	euxassay_013686_07	euxassay_013686_08	euxassay_013686_09

euxassay_013686_10	euxassay_013686_11	euxassay_013686_12	euxassay_013686_13	euxassay_013686_14

euxassay_013686_15	euxassay_013686_16	euxassay_013686_17	euxassay_013686_18	euxassay_013686_19

euxassay_013686_20	euxassay_013686_21	euxassay_013686_22	euxassay_013686_23	euxassay_013686_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13024_wholemount_strong.wlz

13024_wholemount_moderate.wlz

13024_wholemount_weak.wlz

13024_wholemount_possible.wlz

13024_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13024_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 13 weak expression: see section 11 12 14

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 13 weak expression: see section 11 12 14

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 09 10 11 15 16 17

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23

rest of cerebellum marginal layer

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 14 17 18 19 20 21 22

rest of cerebellum ventricular layer

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 17 19 20 21 22

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 10 11 13 14 weak expression: see section 08 12

cochlea

strong

regional

strong expression: see section 02 03 04 05 07 08 09 18 21 22 23 moderate expression: see section 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8677
Entity Detected:	Brip1, BRCA1 interacting protein C-terminal helicase 1 ( MGI:2442836)
Sequence:	sense strand is shown >T8677 TGTACCTTGGTACCTGGCAGCGTCGGAACAGCCGTGAAGCACCTAAAGTAGAGAAGAAGGCAGAATGTCC TCAGTGTTGTCTGACTACACCATCGGTGGGGTGAAGATCCACTTTCCCTGTAGAGCGTATCCAGCACAGC TTGCGATGATGAATTCTATTGTCAGAGGACTAAACAGCAGCCAACACTGTTTGCTGGAGAGTCCCACAGG AAGTGGAAAAAGTTTAGCCTTACTTTGTTCTGCATTAGCATGGCAGCAATCTCTTAGTGAGAAGCCAGTG GATGAGGGCCTGAATAAAAAGCCTGAGGCACCACCTTCATGTTCTTGTGCATGCCATTCAAAGAATTTTA CCTACAGCGACACAAACCTGGACACTTCACCTCATTTCAATAGTCCAAGCAAACCATCTTCTGGAAGAAA TGGAGTCTCAACTCCCTGTCAAGACTCTCCTGAAAAAAATACTCTGGCTGCAAAGTTATCTGCCAAGAAA CAGGCATCTATACACAGAGACGAAGATGATGATTTTCAGGTAGAGAAGAAAAGAATCCGACCCTTAGAAA CTACACAGCAGATAAGAAAACGTCATTGTTTGGAAAAGGACGTCCACCATGTAGATGCACGACTAGCTTC GGAAAAGAGAGTAAAGCCTGAGTCTCCGATAGGAAAGTCTTTCTCCGACAGGAAAGACTCTTTTCAAAAT GTGGATGGCCTTTGTTCCAGGTGCTGCTGTTCTGCTAAACAAGGAAACAACCAAGAGCCTGCAAATACTG TTAAGAAAGATCACGGGGGACAATGCAAGAGACCTAAAATTTATTTTGGGACGCGGACACACAAGCAGAT TGCTCAGATTACCAGGGAGCTCCGTAAGACAGCTTACTCAGGGGTCCCGATGACTATTCTTTCCAGCAGG GACCACAGCTGTGTCCATCCTGAAGTGGTGGGGAATTTCAACAGAAAGGAGAAGTGCATGGAGCTGCTGG ATGGCAAGCACGGGAAGTCCTGCTATTTCTATCATGGTGTTCATAAAATTAGTAATCAGCAGACACTGCA GCACCTTCAGGGCATGTCCAGAGCCTGGGACATAGAAGAACTAGTCAGCCTCGGGAGGAAACTGAAAGCT TGTCCCTACTATACAGCTCGAGAACTGATAGAGGATGCTGACATAGTGTTTTGTCCCTACAACTACCTTT TAGACTCACAAATAAGGGAAACTATGGATATAAAGCTGAAAGGACAAGTTGTCATTTTAGATGAAGCTCA CAACATTGAAGACTGTGCTCGGGAATCAGCAAGCTACAGTGTAACCGAAGTTCAGCTTAGGTTTGCTCGG GATGAGCTAGATAGTTTGATCAACGGTAACATAAGGAAGAAAAGCCACGAGCCCCTACGAGATGTGTGCT ATAACCTCATCAATTGGTTAGAAACAAATTCTAAGCATCTTGTAGAAAGGGGTTATGAGTCGTCTTGTAA AATATGGAGTGGAAATGAGATGCTCTTAAACTTATACAGGATGGGCATCACCACGGCTACTTTTCCAGTT TTGCAGAGGCATCTCTCAGCTGTTCTTCAAAAAGAAGAGAAAGTCACACCAATCCATGGTAAAGAGGAGG CAATACAAATCCCTATTATCAGTGCATCAACTCAAGTCGTGCTCAAAGGACTCTTTATGGTGCTTGACTA TCTCTTTAGAGAAAATAGCAGATTTGCAGATGATTATAAAGTTGCTATTCAACAGACTTACTCCTGGACA AATCAGATTGCTATTTTTGATAAAACTGGAGTCTTGGCTGTACCAAAAAATAAGAAACATTCACGACAGA AAATTGGAGTTAATGCGCTAAACTTTTGGTGTTTAAATCCAGCTGTGGCCTTTTCAGATATTAATGACAA AGTACGGACCATTGTTCTAACATCAGGAACACTCTCACCCCTGAAATCCTTTTCATCTGAGCTTGGTGTC ACATTTAGCATCCAACTGGAGGCTAACCATGTCATCAGTAACTCACAGGTTTGGGTTGGTACTGTTGGCT CAGGCCCTAAGGGTCGGAATCTCTGCGCTACCTTTCAGCATACTGAAACATTTGAGTTCCAGGATGAAGT GGGAATGCTTCTGTTATCTGTGTGCCAGACTGTGAGCCAAGGAATTCTGTGCTTTTTGCCATCTTACAAG CTATTAGAGAAATTAAGAGAACGTTGGATTTTCACTGGCCTATGGCATAGTTTGGAGTCAGTGAAGACAG TCATTGCAGAACCCCAAGGAGGAGAAAAAACTGATTTTGATGAATTGCTGCAAGTGTACTATGACGCCAT CAAGTTCAAAGGAGAAAAGGATGGAGCCCTTCTCATAGCAGTCTGTCGTGGCAAGGTGAGCGAGGGTCTG GATTTCTCAGATGATAACGCCCGTGCTGTCATAACCGTAGGGATTCCTTTCCCAAATGTGAAGGATCTTC AGGTGGAGCTAAAACGACAATACAATGACCACCACTCAAAATCGAGAGGTCTTCTGCCTGGCCGTCAGTG GTATGAAATTCAAGCATACCGAGCCTTGAATCAGGCTCTAGGCAGATGTATTCGGCACAAAAATGATTGG GGAGCTCTTATTCTAGTGGACGATCGCTTCAATAACAATCCAAACCGCTATATATCAGGACTTTCTAAAT GGGTGAGGCAGCAGATTCAGCACCACTCAAGCTTTGCAAGTGCCCTGGAGTCGCTGACCGAGTTCTCCAG AAGACACCAGAAAGTTACTAATAGATCCAAAAAAGACGAAAAATGTACAAAAGACAATGAGCCTACTCTG GAAGTGGCCTGTTTGGAGGATAGCACTTTTACAAGTGTGTCAGAATCAAGTCATCAGTCACCAGAAAACT CGACAGAAGAAGCAGAAGTGTGTGTCCAGGAGCTTCAGTGTCCCCAGGTGGCTACTAAAAGCCCCTCAGT AGCAAGCCACGGTGTTTCCAGAAGGAAGAAAAGTGATCCAGGACTAAGAGGAGAGTCTCTTCAGACAATG AAAACAGAAAAAAATGAGATTTCCCGATCTTCAAGCCCAACTTTTGGAAAGCAAACAGAGCCAGTCAACT GGCCTATCTTTAATTCTCTAAGACGGCATTTCAATAGTAAAGTAAAAAATTGCACACCAGTACTCAAGTC ATCAAAGAACCGTGCCCCTGGTTCTTCCACTTTCAATAAAACTGCTTTGCCACTCACAGGTAACTGTGTG CCCTCAAACGAAACAGCAGACACATCACTGGGACCTTGCCTTCAGTCAGAAGTCATAATTTCACCAGTGA AGATTGAAGCTACTCCTGCTACGAATTATTCCAAACAGGTGTTCTGCTGTGAGAAAGACCTACTCCCGGA TACTGAATTGTCGCCAGGAACTGAAGAAGCCAAGTGTCCCAGCTCAAACAAAGCTGCTGAAACAGAAGTG GATGATGACTCTGAGTGTTTTACACCTGAACTTTTTGATCCTGTCGATACCAACGAAGAAAACGGTGAAC TGGTTGAAACTGATAGATCAAGTCATAGTTCAGATTGTTTTTCAGCGGAAGAGCTTTTTGAAACTGCAAC TGGATTTGGGCAGAAGTGAAAGTCGAAGGTTGCACAGACACATAGTTGAATGGAGTCACACCAGTGGAAG AGAGCAGCGCTGGCAACTTGTATAAGTGAATTAGATCTGGGGAAACTCTTGAATAGACACGAGGGCCTGT AAGCAGTGGGGGCTCCCTGGGATTAGCTGATGCTGGCGGGCTTTTGTTAAGCCTTGTGTAAATAATAATT TGTATACTGGATAAATGTTATCATTTTAACTTTTAAGATATATTCTTCATTGGAATTCATTATCGGATTT TAAGTATGTTATTTCAGAAATACAGGTTTTATTCTAGAGTTTTAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6401109 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6401109 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13025 same embryo
	EurExpress:euxassay_013686 same experiment
	MGI:4823504 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source