EMAGE mouse gene expression spatial database

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EMAGE:13025

Plk2 polo-like kinase 2 (Drosophila) ( MGI:1099790)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013663. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013663_01	euxassay_013663_02	euxassay_013663_03	euxassay_013663_04

euxassay_013663_05	euxassay_013663_06	euxassay_013663_07	euxassay_013663_08	euxassay_013663_09

euxassay_013663_10	euxassay_013663_11	euxassay_013663_12	euxassay_013663_13	euxassay_013663_14

euxassay_013663_15	euxassay_013663_16	euxassay_013663_17	euxassay_013663_18	euxassay_013663_19

euxassay_013663_20	euxassay_013663_21	euxassay_013663_22	euxassay_013663_23	euxassay_013663_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13025_wholemount_strong.wlz

13025_wholemount_moderate.wlz

13025_wholemount_weak.wlz

13025_wholemount_possible.wlz

13025_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13025_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5879
Entity Detected:	Plk2, polo-like kinase 2 (Drosophila) ( MGI:1099790)
Sequence:	sense strand is shown >T5879 GGACGTCAGACTAGAGAGTAGGGAGAGAGACTGGTGCTCGAGGGACAGGGCTAGCCCGGACGCGTGTCCG CGCCTCGGAGGTGGCAAGTAGGCAGTGTCGGGTGGCGAGGCAACGATGGAGCTCCTGCGGACTATCACCT ACCAGCCGGCCGCCGGCACCAAGATGTGCGAGCAGGCTCTGGGCAAAGCTTGCGGCGGGGACTCAAAGAA GAAGCGACCACAGCAGCCTTCTGAAGATGGGCAGCCCCAAGCCCAGGTGACCCCGGCGGCCCCGCACCAC CATCACCACCATTCCCACTCGGGACCCGAGATCTCGCGGATTATAGTCGACCCCACGACGGGGAAGCGCT ACTGCCGGGGCAAAGTGCTGGGCAAGGGTGGATTTGCAAAGTGTTACGAAATGACAGATCTGACAAACAA CAAAGTCTACGCTGCAAAAATTATTCCTCACAGCAGAGTAGCTAAACCTCATCAGAGGGAAAAGATCGAC AAAGAAATCGAGCTTCACAGACTACTGCACCATAAGCATGTCGTGCAGTTTTACCACTACTTTGAAGACA AAGAAAACATTTACATTCTCTTGGAATACTGCAGTAGAAGGTCCATGGCTCACATCTTGAAAGCAAGAAA GGTGTTGACAGAGCCAGAAGTCCGATACTACCTCAGGCAGATTGTGTCAGGACTCAAGTATCTTCACGAA CAAGAAATCTTGCACAGGGATCTCAAGCTAGGGAACTTTATTATTAATGAAGCCATGGAGCTGAAGGTGG GAGACTTTGGTTTGGCAGCCAGACTGGAACCACTGGAACACAGAAGGAGAACAATATGTGGAACCCCAAA TTATCTCTCCCCCGAAGTCCTCAACAAACAAGGACACGGCTGTGAATCAGACATCTGGGCCTTAGGCTGT GTAATGTATACGATGCTGCTAGGAAGACCTCCATTCGAAACCACAAATCTGAAAGAAACGTACAGGTGCA TAAGGGAAGCAAGGTATACCATGCCGTCCTCATTGCTGGCCCCTGCTAAGCACTTGATAGCTAGCATGCT GTCCAAAAACCCAGAGGACCGCCCCAGTTTGGATGACATCATTCGGCATGACTTCTTCCTGCAGGGTTTC ACTCCGGACAGACTCTCTTCCAGCTGTTGCCACACAGTTCCAGATTTCCACTTGTCAAGCCCAGCCAAGA ATTTCTTTAAGAAAGCCGCAGCCGCTCTTTTTGGTGGCAAGAAGGACAAAGCAAGATATAACGACACACA CAATAAGGTGTCTAAGGAAGATGAAGACATTTACAAGCTTCGGCATGATTTGAAGAAAGTGTCGATAACC CAGCAGCCTAGCAAACACAGAGCAGACGAGGAGCCCCAGCCGCCTCCCACTACTGTTGCCAGATCTGGAA CGTCCGCAGTGGAAAACAAACAGCAGATTGGGGATGCAATCCGGATGATAGTCAGGGGGACTCTCGGCAG CTGCAGCAGCAGCAGCGAATGCCTTGAAGACAGCACCATGGGAAGTGTTGCAGACACAGTGGCAAGAGTC CTTCGAGGATGTCTAGAAAACATGCCGGAAGCTGACTGTATCCCCAAAGAGCAGCTGAGCACGTCCTTTC AGTGGGTCACCAAGTGGGTCGACTACTCCAACAAATATGGCTTTGGGTACCAGCTCTCGGACCACACTGT TGGCGTCCTTTTCAACAACGGGGCTCACATGAGCCTCCTTCCGGACAAAAAGACAGTTCACTATTATGCG GAACTTGGCCAATGCTCTGTTTTCCCAGCAACAGATGCCCCTGAACAATTTATTAGTCAAGTGACGGTGC TGAAATACTTTTCTCATTACATGGAGGAGAACCTCATGGATGGTGGTGATCTCCCGAGTGTTACTGACAT TCGAAGACCTCGGCTCTACCTCCTGCAGTGGTTAAAGTCTGATAAAGCCTTAATGATGCTCTTCAATGAC GGCACATTTCAGGTGAATTTCTACCACGATCATACAAAAATCATCATCTGTAACCAGAGTGAAGAATACC TTCTCACCTACATCAATGAGGACAGGATCTCTACAACTTTCAGACTGACGACTCTGCTGATGTCTGGCTG TTCGTTAGAATTGAAAAATCGAATGGAATATGCCCTGAACATGCTCTTACAGAGATGTAACTGAAAACAT TATTATTATTATTATTATAATTATTTCGAGCGGACCTCATGGGACTCTTTTCCACTGTGAGATCAACAGG GAAGCCAGCGGAAAGATACAGAGCATGTTAGAGAAGTCGGACAGGTGGTGGTACGAATACAATTCCTCTG TGGCCTGCTGGACTGCTGGAACCAGACCAGCCTAAGGTGTAGAGTTGACTTTGGACAATCCTGAGTGTGG AGCCGAGTGCAGTTTTCCCTGAGATACCTGTCGTGAAAAGGTTTATGGGACAGTTTTTCAGAAAGATGCA TTGACTCTGAAGTTCTCTCTGTTGAGAGCGTCTTCAGTTGGAAGACTTGGAACTGTGAATACACTTCCTG AAGGGGAGGGAGAAGGGAGGTTGCTCCCTTGCTGTTTAAAGGCTACAATCAGAGCAGCTTTTGGCTGCTT AACTGTGAACTATGGCCATACATTTTTTTTTTTTTGGTTATTTTTGAATACACTTGTGGTTGGAAAAGTG CATTCCTTGTTAATAAACTTTTTATTTATTACAGCCCCAAGAGCAGTATTTATTATCAAGATGTTCTCTT TTTTTATGTTGACCATTTCAAACTCTTGGCAATAAAGAGTATGACATAGAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:3156743 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3156743 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13024 same embryo
	EurExpress:euxassay_013663 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source