EMAGE mouse gene expression spatial database

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EMAGE:13037

Plekha1 pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1 ( MGI:2442213)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013789. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013789_01	euxassay_013789_02	euxassay_013789_03	euxassay_013789_04

euxassay_013789_05	euxassay_013789_06	euxassay_013789_07	euxassay_013789_08	euxassay_013789_09

euxassay_013789_10	euxassay_013789_11	euxassay_013789_12	euxassay_013789_13	euxassay_013789_14

euxassay_013789_15	euxassay_013789_16	euxassay_013789_17	euxassay_013789_18	euxassay_013789_19

euxassay_013789_20	euxassay_013789_21	euxassay_013789_22	euxassay_013789_23	euxassay_013789_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13037_wholemount_strong.wlz

13037_wholemount_moderate.wlz

13037_wholemount_weak.wlz

13037_wholemount_possible.wlz

13037_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13037_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 07 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 08 18 20

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 18 19 20

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

cervico-thoracic ganglion

strong

regional

strong expression: see section 10 11 12 17

cervical ganglion

strong

regional

strong expression: see section 09 10 17 18

thoracic ganglion

strong

regional

strong expression: see section 13 14

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 16 17 18

neural retina

strong

regional

strong expression: see section 01 02 03 04 23 24

anterior naris epithelium

strong

regional

strong expression: see section 13 14 17

external naris epithelium

strong

regional

strong expression: see section 13 14

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 17

renal cortex

strong

regional

strong expression: see section 05 06 07 08 09 10 17 18 19 20 21

seminiferous cord

strong

regional

strong expression: see section 05 06 07 08 20 21 22

left lung

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14

right lung

strong

regional

strong expression: see section 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6489
Entity Detected:	Plekha1, pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1 ( MGI:2442213)
Sequence:	sense strand is shown >T6489 GCGCCGCTCCCGGCAGCTGGGTGCCTGAAGGAGCCGGGACCGCGGTGGCGCGGGTGCTCCGGGCGGAGGC CGCGTGTAATGTTCAAGCCCAGAAATGCCTTATGTGGATCGACAGAATCGCATCTGTGGATTTCTAGACA TTGAAGAAAATGAGAACAGTGGGAAATTTCTTCGACGGTATTTCATCCTGGATACCAGAGAAGACAGCTT TGTATGGTACATGGATAATCCACAGAACCTGCCTTCAGGATCGTCACGGGTTGGAGCCATTAAACTTACC TACATTTCAAAGGTACCAAAGCAGTCAGACTCACAGCCGGCCTCCGACAGCCTGAGTCGCCAAGGTGACT GTGGTAAGAAGCAAGTGTCTTACAGAACTGACATTGTTGGTGGTGTGCCCATCATCACGCCGACGCAGAA AGAAGAAGTAAACGAATGTGGTGAGAGTCTGGATAGAAACAATTTGAAACGGTCACAGAGCCATCTTCCT TACTTTGCTCCTAAGCCACCTTCAGACAGTGCAGTTATCAAAGCTGGGTATTGTGTGAAGCAAGGAGCGG TGATGAAAAACTGGAAGAGAAGATATTTTCAATTGGATGAAAACACAATAGGCTACTTCAAATCTGAACT GGAGAAGGAACCTCTGCGGGTGATACCACTTAAAGAAGTGCACAAAGTCCAGGAGTGCAAGCAGAGTGAC ATAATGATGAGGGACAACCTGTTTGAAATCGTGACGACATCTCGGACTTTCTACGTGCAGGCTGATAGCC CTGAAGAGATGCACAGTTGGATTAAAGCAGTCTCTGGCGCCATCGTAGCACAGCGGGGACCTGGCAGGTC ATCCTCTTCTGAGCATTCCGGCTGGCCTTCAGAACCCAAATTCACTGTCCGTCCTGCCGGGGCCGCAGCC ACCGCCTCACATTCCCCAGCCTCTCGCAGCCACTCTCTGGTCTCAGGCTGTGCCATGGAGAAGCGAGGAG TTTACCAATCTCTTGCCAAGGTCAAGCCAGGGAACTTCAAGGTCCAGACTGTCTCTCCAAGAGAGCCAGC TTCCAAAGTGACTGAACAGGCTTTGCTCAGACCTCAGAGTAAAAACGGCCCTCAGGGAGAAGATGGCGAG CCAGTGGACTTAGATGACGCCAGCCTGCCAGTCAGCGATGTGTGAGGAGGCTCTCGTGGGCTGCCTGCGC CACTCACCCTCAGTGTCCTTCCGTGAGGCTTCTCGCCAAAGATGATAGAGGGTGAATGATCTCAGTGCAT ATGTCATACTGCCTTGTGGGTGGACTCTAAGTTGGCAAACCAAGGATTTAGGGAGTGGCCTAGTCAACAT AATTCTTTAAGAAAGGAAGGAGAAAAGCTGTGTCCATAGTGACTGTTTGAAGCTTTATATGTTTGGGTGG TGAAAATGTGTGTGTGTGTTTTTTTTTTTCTTAAGTGACTTTGAAATTTTAAATAACAATTTAAAACATT AAAATTTCTTATTATCTTGGTCATTTAAACCTGCTAATATGACTCCCTTTGAAATATTCAATATTTACAC ATTCTTATCAGCTAAAATTATCAAGTGGGCCATTCCCAGATGAAAATTTCTAATCTCATGAGGTCTGTGG TGCTGCAGAATTTATACTGCAATGTGGACTGTTTAGAATTCTAACCATTTATGATATTCTGGCTGTCCAG GTGAGTGGAAATTTTTGCATAGATTTGACTAGATGATTGGTGTAGGTGGCAGTGGAACAGGGCAAGCAGA AGTGGGGAGACTGGAGGTCAGCTATAGCTAAGATTGTTATTTCTCTGGGGAGGGGACTTTTTTTATCTTT ATTTTTATTTTGGAGATTGGATTTTTCTGTGTAGCTATGGCTGTCCTTGAATTCTGTCTGTAAACCAGGC TGCCCTTGAACTTGTGATCCTTCTGCCTCAATTGCCTCCCAGATTATAGGATTATGTCACCAACTTGCAG CCTGAGACACTTGTTTTAAAAAGCTCAGTGTTTTATTTCAGTAAAAATTATTTGGTCATAAATTTTGGGA AGAACTGATTTCTTTTTTTAAATCAAAGTTTTGTAAATAGATCTTACTTCTGAATTTGAATCGGTGGAAG ACCTCAGTTCACATGCTAAGTCAAAACGGCCCTTAGTCACGACCTTGTTGTGGCCCCTCCCTTGTCATCA GTAGTAACCTATACTCAAGTGCCTGTGTCTTCTCATCTCACTTAGGATTGTACCCCAAAGTCCTACATGA TTGGCTTTAAATGTCCGGGAAAACTAATTTCAGTAGCGTAGTTTGATAGGTTAAATGAGATAGCATTTTT ATATCAGAGACACCAGAATGTTGGTGTTCAACTACCTGTGCAATATTTATGTCTTGAAAACCAGATCTGA GTATGCATTGAACAGTGGGGATATTATAAACAGAAGCGCCTCTCTGAAGAACCCGGTTTCCGGTGTTGAG TGATAGACTGGTTTGAGCGAGTACTGTTTTGTGGCGACATCTTAGGTGTTTCCTTTCTCCCAGGCTGCTG CTCTGTGGTATTTCCAGTCTCAGACTTGTGTTGTGGAGTCATGGTGTTAGTGTTCTGCCTTTTTATGTTC TAAGACTACTTGTTAGAGGACGGAAGATTTGCTACAGTGGTCTAGAGTTAAAATGTGTTCTTTAGTTGCC CAGCACAAGAACGTACCTTTTGTATTCATAAGCTAATATGTTAGAGTTCTATGTAGTTTTCTCTTGTGCA CCTGCATCAGAAAACCATAGGACAACAAGGTGGCTAGGCAGTTATCACCCCTCTGCTGCCGTATTCATCA CCCAGGCTCTCTTACAAGTTCTTTGTTAATGCACAAGCGTAGGGACCCTTGTACAGACTCTGGCAGTATT GATGAGCCTGGCGCTGTTTGCTTTGGTGCAGCCTCCACAGTGCGCGTTCATTTTATCATATTTTGGTATC GGTAATTTGATATATTTCATGGTGGCAAAATATTAGCTCTATTTTGGGACTTTTTCTAGACTTACATTTA TTCAAATGCATAGGAATATGTCCTTGTGATTATCAGGATCTCCTAATATTTATCTCAATTCTTTTGTAAG CTTGTGGAAATTATTTAAGTATTTTAAAGCATATTTTCTTGTAAATATGTCCTATCCGAAGTGTCAGCAG TTACGTTAGTTAATACCGTGTTATCTGCTGTATTTTCCCTGTGTGTATAATGTATCTCCTTTCAGAACAG GAATGTGCCTCCTAATGTTTACTGTTACCCTGCTGTCTCTGTATGTCCAGTCGGTGGAACACTGTAATGA CTTGAGAATAAACTGCCCAGAGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3589001 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3589001 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13040 same embryo
	EMAGE:13038 same embryo
	EMAGE:13039 same embryo
	EMAGE:13041 same embryo
	EurExpress:euxassay_013789 same experiment
	MGI:4827262 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source