EMAGE mouse gene expression spatial database

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EMAGE:13045

Thsd7a thrombospondin, type I, domain containing 7A ( MGI:2685683)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013737. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013737_01	euxassay_013737_02	euxassay_013737_03	euxassay_013737_04

euxassay_013737_05	euxassay_013737_06	euxassay_013737_07	euxassay_013737_08	euxassay_013737_09

euxassay_013737_10	euxassay_013737_11	euxassay_013737_12	euxassay_013737_13	euxassay_013737_14

euxassay_013737_15	euxassay_013737_16	euxassay_013737_17	euxassay_013737_18	euxassay_013737_19

euxassay_013737_20	euxassay_013737_21	euxassay_013737_22	euxassay_013737_23	euxassay_013737_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13045_wholemount_strong.wlz

13045_wholemount_moderate.wlz

13045_wholemount_weak.wlz

13045_wholemount_possible.wlz

13045_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13045_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 01 24

forelimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 01 24

forelimb digit 5 mesenchyme

moderate

regional

moderate expression: see section 24

hindlimb digit 1 mesenchyme

moderate

regional

moderate expression: see section 23 24

hindlimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 23 24

hindlimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 02 03 23 24

hindlimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 02 03 23 24

hindlimb digit 5 mesenchyme

moderate

regional

moderate expression: see section 01 02 23 24

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 07 08 09 10 11 12 14 15 16 17 18 19 20 24 moderate expression: see section 03 04 05 06 21 22 23

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 06 07 09 10 11 12 13 14 15 16 17 18

medulla oblongata alar plate marginal layer

strong

regional

strong expression: see section 08

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

rest of cerebellum mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 09 10 11 12 13 14 15 16 17 18 19 20 21

rest of cerebellum marginal layer

strong

regional

strong expression: see section 08

pons mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 08 17 18

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 09 16 17 18 19

spinal cord mantle layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 15 16 17 18

neural retina

strong

regional

strong expression: see section 01 02 03 23 24

nasal cavity olfactory epithelium

strong

single cell

strong expression: see section 08 09 11 12 15 16 18 19

stomach

moderate

regional

moderate expression: see section 04 05 06 07 08 09

midgut

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

kidney calyx

strong

single cell

strong expression: see section 07 08 17 18

thyroid cartilage

moderate

regional

moderate expression: see section 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37848
Entity Detected:	Thsd7a, thrombospondin, type I, domain containing 7A ( MGI:2685683)
Sequence:	sense strand is shown >T37848 GAAAACCACAGAAGGGAAACAGACCCGAGCCCGGTCAATTTTGGCCTATGCAGGTGAAGAAGGTGGAATT CGCTGCCCTAACATCAGTGCTTTGCAAGAGGTGCGCAGCTGCAATGAGCATCCTTGCACTGTGTACCACT GGCAAACTGGTCCCTGGGGTCAGTGTATAGAGGACACATCGGTGTCATCCTTCAATACGACCACAACATG GAATGGGGAGGCCTCATGTTCTGTGGGCATGCAGACACGAAAAGTCATCTGTGTGCGAGTCAACGTGGGC CAAGTGGGACCTAAGAAGTGTCCTGAAAGCCTTCGGCCTGAAACAGTGAGACCCTGCTTGCTTCCTTGTC GGAAGGACTGTGTCGTGACCCCATACAGTGACTGGACTCCATGCCCCTCTTCATGCAGAGAAGGAGACTC TGGAGCCAGGAAGCAGTCTCGACAGCGAGTCATCATTCAGCTGCCAGCCAATGGCGGCAAGGAATGCTCA GACCCACTATATGAAGAGAAGGCCTGTGAAGCCCCTCCCACATGTCACAGCTACAGGTGGAAGACACACA AATGGCGCAGGTGCCAGTTAGTTCCTTGGAGCATTCAACAGGATGTTCCTGGAGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 79238. Forward Primer - name:079238_F_cDNA_LOC330267, sequence:GAAAACCACAGAAGGGAAACAG; Reverse Primer - name:079238_N_SP6_cDNA_LOC330267, sequence:GCTCCAGGAACATCCTGTTGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13044 same embryo
	EMAGE:13042 same embryo
	EMAGE:13043 same embryo
	EurExpress:euxassay_013737 same experiment
	MGI:4828706 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source