EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13046

Frs2 fibroblast growth factor receptor substrate 2 ( MGI:1100860)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013784. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013784_01	euxassay_013784_02	euxassay_013784_03	euxassay_013784_04

euxassay_013784_05	euxassay_013784_06	euxassay_013784_07	euxassay_013784_08	euxassay_013784_09

euxassay_013784_10	euxassay_013784_11	euxassay_013784_12	euxassay_013784_13	euxassay_013784_14

euxassay_013784_15	euxassay_013784_16	euxassay_013784_17	euxassay_013784_18	euxassay_013784_19

euxassay_013784_20	euxassay_013784_21	euxassay_013784_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13046_wholemount_strong.wlz

13046_wholemount_moderate.wlz

13046_wholemount_weak.wlz

13046_wholemount_possible.wlz

13046_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13046_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9198
Entity Detected:	Frs2, fibroblast growth factor receptor substrate 2 ( MGI:1100860)
Sequence:	sense strand is shown >T9198 CTGGAGAGAGGCCTTGTAGACACAGGCGCCGAGGCTCCGAGCAGGGGGTCAGGTCGCGGGGAGAGTGAGG AGCTCCGCGGCTGCCACCCCGCGCCCCTCGCAGCGCCTTCGCGCCGACGAAATGGCTACCAAGAAGTAAA TGCCCAGACGTAATGGTGATGCAGGTGTCATGAAGTAAGATGCAGTATAGCATCCATCCAGATTAGGACA AAATTATATCTGTTTTTCTCAGAAAAGAATTCCAAAAGACTTTTTGAAGGAAGACATCATCGAGTTAAAG TACCAAACGAAGACCTGGTATTTTGAAATATGATTAATCTCCTGATGCAGCATCAGAGGCTAACAATATT AATGGCCAGATCTAGTGCTCACATGGTCTTCTGAAGAAGCCATGGGTAGCTGTTGTAGCTGTCCAGATAA AGACACTGTCCCAGATAACCATCGGAACAAGTTTAAGGTCATTAATGTGGATGATGATGGGAATGAGCTA GGCTCTGGTGTGATGGAACTCACAGACACAGAGCTGATTCTGTACACCCGGAAACGGGACTCGGTAAAAT GGCACTACCTCTGCCTACGACGATACGGCTATGACTCAAATCTGTTTTCTTTTGAAAGTGGTCGAAGGTG TCAGACTGGACAAGGAATTTTTGCTTTTAAGTGTGCCCGTGCAGAAGAATTATTTAACATGTTGCAAGAG ATTATGCAAAATAATAGTATAAATGTGGTGGAAGAGCCGGTTGTAGAAAGGAGTAGTCATCAGACAGAGC TGGAAGTCCCTAGGACACCTCGGACACCTACAACTCCAGGGCTCGGTGCTCAGAACTTACCTAATGGGTA TCCCCGCTACCCCTCGTTTGGAGACGCTTCATCCCACCCCTCAAGCCGGCATCCTTCGGTGGGAAGTGCT CGCTTACCTTCAGTCGGTGAAGAGTCCACACACCCTTTGCTCGTGGCTGAGGAACAAGTACATACTTATG TTAACACTACAGGTGTGCAAGAGGAGCGAAAAAACCGTGCAAGTGTGCATGTTCCCCCAGAGGCAAGGGT TTCTAATGCTGAGAGCAACACACCAAAAGAAGAGCCGAGTAACCCCGAGGACAGGGACCCGCAAGTGCTT CTTAAGCCTGAAGGAGTCAGGTTTGTGCTAGGACCAACCCCTGTCCAGAAGCAGTTAATGGAAAAGGAGA AACTGGAGCAACTGGGAAAAGACCCAGTCAGTGGGAGTGGAGCGGGCAACACGGAGTGGGACACCGGCTA TGACAGTGACGAGCGCAGAGATGTGCCCCCTGTCAACAAACTGGTGTATGAAAATATAAACGGGCTATCT ATTCCCAGTGCCTCAGGGGTCAGGAGAGGTCGTCTGACATCTACCAGCACCTCAGACACCCAGAATATCA ACAACTCAGCGCAGAGAAGACCTGCACTATTAAACTATGAAAATTTACCATCTTTGCCTCCTGTTTGGGA AGCCCGCAAGCTCAGTAGGGATGAAGATGACAATTTAGGACCAAAGACCCCATCTCTAAATGGCTACCAT AATAATCTAGATCCAATGCATAACTATGTTAATACAGAGAATGTAACAGTGCCGGCAAGTGCTCACAAAA TAGACTATTCGAAGCGCAGGGATTGTACACCGACAGTCTTTAACTTTGATATCAGGCGACCCAGTTTAGA ACATAGGCAACTCAATTATATACAGGTGGATTTGGAAGGGGGCAGTGACTCTGACAACCCTCAGACTCCA AAAACGCCGACCACTCCCCTCCCGCAAACCCCCACGCGGCGCACAGAGCTGTACGCTGTGATAGACATTG AGAGAACTGCTGCTATGTCCAACTTGCAGAAAGCGCTGCCTCGAGACGACGGTACATCTCGGAAGACGAG GCACAATAGTACTGACCTGCCCATGTGAGCCGGGAAGCACTGCGTCCTTCTCCGCAAGGCGTTCAGAGCG AAGATGCAGTGCCTCCTCTTCATGTCTACACTAGCTCCTTTATAGACTGGTCTTCTGCTCTGAATATTTC ATCTTTAAAGGGAATTGATGCAGAGCAGGCACTCCTTAGAACACTAATCTCCTGATAGCTACTCACTGTC CAGCAGCACCCCATTCTCACAGTGCCTACTTACCACGAGAATTGAAGTGAATGCCGTGCTGGGTGACCTG TCCTCAGCATCCTGGGGGATGCATAGCTTTCATGTCTGAGTTGTGGTTGGCGTTTGAACCTTTTATAAAG TCTCTTGATAATGCGCATTGCTAACATAACTCTGGGCCCTGAGAGGAACCCTCATAGATCACTGTTCACA GGGTATGGTCGCCAGCATGGAACGTGAGGATTTTATTTCATTAGTTAAAGGCTTTTTGTCTTGGGCCAAA TTCGTGTCAAAGAACAAGGAGAACCACACTTCCTTTTGTACCAGTCAGCACCTCTGTCTTGAGTGTGCTG TGATTGGAAGGCAGCCAGGAGACAGCTCTGTGTCCCCACCTCCTGGTGTCATCTGAAGGGCGTGTCCAGA TAGCATCTTACTCAGAGTTAGGATGGGCTTAGGAGTCAGTCTCAGGTCACTTTACCCAAAAGCATTTGAA AGTCTACCCCTGATCTCTCGGTGTGTCTGCGGTAGATTACTGACAGCACACATGTGTCTGCAGACACTCG TGCCATTCAGTCCCATGGAGCTTCAGTTTCCCCTTTGGTCTTTGGGTTGTCTTACCTTGTATGTAATGCT TTTTTCAATTTACAACACAATGTTTGTGTATCGTCTTGGAATAGGATTGTGTGTTTGCTGTTACTACTTA GGCTGCGTTTTTGTTGTTACGGTCCTGAAGGTCCATTTGGGACTGACTGTTATTCTCTAGCTGGGTTGCC CTTGTCCAGTATTTATTTATCTCCTGCTTGCTTTTCAAGTTGATGAAAAAAATCGACGTTTGGGTTTTAA AGTTCATTTGTGTCCATGGGTGATCTCTTTAGTAGCTGAGAAGGAAAGGAAGAGACTCTTAAACATGTGA GTCCCCGAAGGTCCTGTCCTCGCTGGGGCGCCCTGCGCTGCACTTTAGCAGGGGTTGTCTCAGCTCCATG GCTGCAGGCCCGCTCTCCCTGCTCTGCTGTCCTCCCACGTGGAGCTGGGCTCTGATTACCCTGTGATGCT GGTGAGCTGTTAATATTGTTGCCAGCATAACAGGAACAGGGAAGTCTTAAAGAGGCGCCGTTATGTCATA GCGTGAAGTGAAGAAAGGCCACTCAGACTGATGTGCTCCCAGACCGACCACTGGACAGTACAGGGAGGGG CAGCCTGCGGGATAGGTTATCTTTTCTCTAAAGTTGGAGACATTCCTGGTTATGATCTGGATATACCAGG TCAAGAAAAGGGTTCAGTCATCGGCAGGCAGTGTATTTAGGAACTCATCCAGAGCAGCGGCTGGATTTGC AGAACAGTAGTGCGGGGCCATAATATCTAGATGTTTCATCATCTTCAGCGCCATTGCTTCATAGCATAGC AGACGCTTCCTTTCCTTTCCCTCTGCGAACTAAAATGTTTGCTGTTCTCTTCAAGAGAATAGTGGATACA TGTGCAGTTTACATATTTAATACACTGTTCCATTCGTCCTTTCTTCTGAGTAGCTTGCTAATTGTGCCAA ATTTCAGTGGTGGTTTTGTGGTGTGGGGTAAGGACGCTTATGGGAAGGGCGCAGGTCCAGACAGAATTAG CCCTCCACCTCAGAGGGCTGGTCTGCGCGGCCGTCTGCAGGCTGGGTGCTTTGTTCTTTCTCTGTACTCT TCACTTTTCCCTTCAGATTTGAATTAGATTTTATATTTTTTAGCTGACTAATATCATCATTACAGCTATG CTTTAAGTAGTATATGGCTTAAGAATGCAAATGGGACCAATTTGTCTGCTAAGAATTTGATTTTAGGGTA CAATATGAATATTAGGAAGAGGTAAACAGGTGTGTTAATTTCTAGATATCTTCTGGAAACCACCTTTGTT TCTGTTTAACCAGTCCTGTAGAATCCCTGTGTCCTTTACAGTCGTCTCATGATGGGCATTTAGCTCTGTC CTGGAAGAATGTACCTGCTACTATTTGGTGAATTTTAGAGTGAGATATTTTATTGGCATATTTGTAGCCA TATGTCTCAGATTTTCTGAATCAAACCTAATGTCAGATCATTGGTTTTGTTGGTTAGACCATGTTACCCC ACTTTCTGGTTTTGGAAATATAATCCTTGCTCCCTCCTTTTCTCCCCAGATAAAAACCCTTGCTTAGTCC ATATAGTCCATATAGATCCTAACACTTCTACAGACCAGGGCTGGAGACTGATAGGGCATCACTAGTGTTT CCTGACTGTTGTGTCCCAGTTTCATCTTCCTTTGAAAAGTGAAAATATGGTGCCTTCCCTCCCTCCCTTC CTCCCTCCCTCTCTCCCTTCTTTCTCCCTCCCTTCCTCTCTCCCTCCAAGAAGACGGGCAAATCGTTCCT TGTTCTCCCACTGCTGTGGAGTGATGAGACACGCACTTTACTCTTGAAGACTCAGCAAAAAGACTCACTT CTCAGCATATCCATAATAGACCACATTTCGGACTTAGGAAACTTTGCCAAGCAGTCTCTGTCATTACAGA TATTAATGTTGCCTCCCCCACCCCCCCACACTATGTTGTAAGTCAAACTGTGTTTGTCTGTGCTCCCCAC TGCGCCTACCCTAAATCTGTGGGTGTGCACAGCATATAAAATGTACCTCACTAAAGTTCTATTGCAAATG GGACGGGGCCTTATGTGTTCATCATTTCCCAGCAATGTGCCACCATGTATTTGCCTCAAGGCAAAGGTTT AACAAGCTAACAAGTACTTCCCAGTTCCCCTGTGCTGTTTTCTACCCACAATACCCAAGTGTTTTGTGCA ATGTGTAGTGTGTATGTATAAATATATATATATATATTCTTTAGAGACATCATTCAGAAGTAATGTATTG GCATCTCATTCATATTTCTGATGTGAAGTATATGGTACTATTTACTTTTTCCTTAATGTTTGCCAAATTA GAATCAAGACGTTGGTACGAAATTACAGCATGTGTAGAAACCATTTTGGCTTGAAGAGTTAACCTGAAAT CCTTTCCCCCGTCAGTGTGCCCTGCACAGACTAGTGCCCTGCCGGCTCGCTGCCTGGTTCCCAACCCTGC GTCCTCAATGTTCAGGGTGTAACACAGTTCTCTCATAGCATCCTATCATTGAAATTGTCTGACAGTTGGA CAATGAAGCCTGGAAACAGGCTGGTACAGTGTACAATAGTCACCAGGGGCCGAGTTTGCATTAGTAAATG CAAAATAGATGTTTTATAAAGGACAGACTTTGTGTTATGTTTCTATTTTCATTACATTGTATAATAATGT AAGATTATTGTATGTTCTAATTTGCATTATAAATGTTTTTTTCCTACATAAAGGCATAAATATAGCAACT TTGTATAAAGGTAGCTTATTAGATTTTAATTTTTCTTTTATAAAAGTTGTCTACAGTGGGACTACCATTG CCAAATTGTATATGAGATATGAATCTTCCCCTATGGTTAATTTCTTTTCTAAACATTCCATATTTCTCTA ATGAAAGACGTGATACTGTACTATGCATAAATTGTGTTTTAATGCTGTTTCATATCAGCAGGTTAATCTT GGGTTGGCAAACGTAACCACTGTTATTACAATAAAACCTTGTGTGTGTAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6830555 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6830555 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13049 same embryo
	EMAGE:13048 same embryo
	EMAGE:13047 same embryo
	EurExpress:euxassay_013784 same experiment
	MGI:4824930 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source