EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13047

Nckap5 NCK-associated protein 5 ( MGI:2686394)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013783. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013783_01	euxassay_013783_02	euxassay_013783_03	euxassay_013783_04

euxassay_013783_05	euxassay_013783_06	euxassay_013783_07	euxassay_013783_08	euxassay_013783_09

euxassay_013783_10	euxassay_013783_11	euxassay_013783_12	euxassay_013783_13	euxassay_013783_14

euxassay_013783_15	euxassay_013783_16	euxassay_013783_17	euxassay_013783_18	euxassay_013783_19

euxassay_013783_20	euxassay_013783_21	euxassay_013783_22	euxassay_013783_23	euxassay_013783_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13047_wholemount_strong.wlz

13047_wholemount_moderate.wlz

13047_wholemount_weak.wlz

13047_wholemount_possible.wlz

13047_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13047_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18

otic capsule

strong

regional

strong expression: see section 05 06 07 08 09 17 18 19 20

cochlea

strong

regional

strong expression: see section 07 08 09 17 18 19

retina

strong

regional

strong expression: see section 01 02 03 04 05

anterior naris epithelium

strong

regional

strong expression: see section 14 15 17 18

external naris epithelium

strong

regional

strong expression: see section 14 15 19

left lung

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12

right lung

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9391
Entity Detected:	Nckap5, NCK-associated protein 5 ( MGI:2686394)
Sequence:	sense strand is shown >T9391 GTGCGCGCCTGCTGCTGCTGCCGCGCCTGCCTTACCACCGGCTTGGGGGCATCTTTTTAGCTCGAGATTC CCAGGGAAGTGGCACTGCTGTCCCCAGGCGGAGCGGGGGAGAAAGGTCCTGTGGCCCAGATTGCACTGGG ACACCGGGAGGCAGGCGCGAGCTGGGGACAGGAGGCGTGCGACACAACAAAAGCCTAGGTGCCCGGGCTG CGGAGCACGCGCGCGCGCCGACAGGCTTGACATGGCAAGTTTGTCAACTGTGGGCTCCGAGGCCAGCTCC TTGCTGCCCCCCAAGTGACGGCATTGCAGGGTCTGCCCTCCAAGGCGGGCAGCCCCCACTACGACCTGGG CCCTCTGCAAAATGCTCTCCCGCAGGCCAGGGAGAAGCTGGCTGTGGCCAGGCTGCAGAGAGAAGTTGCC CAAAGGACAAGCCAAGGGGCCATGCACGAGAAGCTGATACATGAGTTGGAAGAGGAACGGCACTTGCGGC TACAGAGTGAGGAGCGGCTGCGGGAGGTGACCCTGGAGTCAGAGCGCAACAGGATCCAGATGCTCGGCCT GCAGCAGCAGTTTTCCAGAATGGAAGAAACTGTCCGAAGTCTGTTGCAGAGCCAAGGATCTCCAGAGCAG AAAAAGGAAGAACCTGCCAAGATCACAGCCTACCAGGAAGGACTACCTGATGAAGAGAGAAAAGAGCAAG CAGCACTGGGTGATCTCCATGTGGTGGCAGCTGATGAGGACTCAAGAAGCGAGCACAGTAGTGTGGAGGA GGGAAAGGAAGACAGCAGACTGCTCCTGAAGAGACTGAAGGCCCTGGAGGCGGAGAATTCAGCACTGGCA CTGGAAAACGAGAATCAGAGAGAACAGTATGAGCGATGTCTTGATGAGGTAGCCAACCAAGTTGTTCGAG CTCTACTCACTCAAAAGGATCTGAGGGAGGAGTGTGTAAAGTTGAAAACAAGAGTGTTTGACTTGGAGCA ACAGAACCGAGCACTCAGCCTCCTGTTCCAACAGCGAGTCAGACCAGCTTCTGATGTGCTTCTCCAGGAA CACCTCCAGAATGCAAAATCAGGAACCCCTGCCTTGAAACACTCTGGCTCAGGGGTTGTCATCCCTGATC ACCTCTGTCCTCGCAACAGCTGCAGCAGTGGCAGTGAGCTATCGCTGTCAAGCACCTGCAGCGAGTACTC CAGTGGCTCCTCCTACACGTGGCAAGACAGGAAGCTCATTGGGAAAAGGCAATCATCGCGGATCTGGGAT AAAAGACTAAGTATTGATTCTTCGCTCCCAAGTGGCTTCGCCAGCCCTGCAGATGAACTACCTCCGACCC GTATCAAAGAAAGCCACATTCTAGAGGGACTGAGGAAGCTACAGAAGCGGAAAGTGTTCCTTGAATCACC ATCATTGGTGATAAGCAAATGGGGTTATAAAGATTGCATGAACTCGAATGAAGGGATCTATTCGCCAGGA ATTAAAACCAGCAGCCTCAAGGAGTGTCCGCCCTGCCAACCGTCTGGCATGGGGATCCCCTGCCAGAATC TCCATCAGACATTCATCTATGATGCAAATTCTCATGAGGATGCACATGAAGAACTCCCATCCTCAGCCTT GCCACTAACATCTCCGAGCCAGGGCTACAGAATACAGGGCAGTAAATTGACACACAGTGTTTCAGACAGT CTGTTTAACTGGGAGCCACATAGGAAGTACTTTGCAGAAGGTACAGCCTCAGTATATACCAGAGAAATGC CTGAAAGGCTAGCTCGTTGTGCTAGCCACTGCCCCTTGGAGAGGAAGCTATGTCCCAGTATACACCTGCC CTGGGTGCAAAGAGACAGGGACCCACACAGCCAAGTTCATGGCCATGTGCCTCTGAACCTGCAGCTCTCA GATACCGATGACAATGAGACCCTAAACGAGCTACACATAGAGAGCAGTGATGAGAGAAGCCCTTCAGACC TGTCCTTGACTGGGGACACCGACAAGTCCATGGAGAACCTAGAGGTGGGATTCAGAAAGTCTGAAGGTGG CTCTCGGGACATGGGAGAAGAAGCATGTCCCACCCACTTAGAGACTAGCCCGAAGGCATTCAGTTTCATC AAGCAACAAAGGGTTGTCAAGAGGACCTCTTCGGAAGAATGTGTCACGGTCATATTTGATGCAGAAAATG GAGAGCCAGTGGAATTCAGCTCTCACCAGGCTGGAGTCGTCACTGTCACCAGGGATGAAATTTCCATCAG CTCAGCCCTTGCTGGGCCCTCAGAGGAACTCACTGAACTTTCACCTCAGGAAATTACTCACTTCCAGGCA GGAGCTACTGCAAGAGACTGTCCTTTTCTGAAGAGACCTGAAGAGGAGACTGCGAAAAACATTCCAAAAG ATGAAAATCATATTCTCACAGCTCCGAATGACTTCTTCAGCTCCCAGACTATAATAACTCAGAATTCCCC AAGACCAAAGCTCGCCAAACCCACACAGGGGGTAGCCTGGCAGAGTAATTCTAGACCTGTTGGAAACATG GGTGTCTACACAAAGCAAGGTCTGACAAAAATCCCTTCCAGAGGCAAGTCTTCACCTCAGAAATCCAAAC TTGTGGAGTCTATGCCCACCATGCTGGTCCCCTCCAGTGCCCTAGTGACTCCTGAAAAGACACCAGCATC CCCTATGGGGAAACTCTCACAATGTAGAAAGGCAGAGAGCCCTGGTTCTCTCTGTGATGTACAGCCAGAA TCACACATTCCAAAACACCCTGCCCAGGTTCCTCATATCCCCAAAATTTCCAATAAGAAGAACTGGGTTC AGTGCCCCAGGAGTCAGACTTCAGGGATCCAGTCACGGCATCTCATGGGGAGAAGTGACAGTAGTGAAAT GAGGTCCAATGGCTGTGTCTCTGCGCCACAGACAAGAATGAGGTCTCCCTCCCCTCCAACCCCTCCAGGG AGATCAGTGTCACTCCTGGTTAGACCCAGTTATGATTACCCACCACTACCATCACCTGCCAAACCTGAAA CTCAGGGCCCTGGAGACGCTGTAAGTCCAGGGTTGAAATCCCCGCTGTTGAAAGGATCCTCTGCTCCTCT TGTCTCTTCCAGTCCAGTGATGTCAGAAATACAGAAGAAACCATCTGTGGCCTCCAGAAAGTCTGCCTTC CCTCCAGCTCTTCCCTCAACCCAAGCGGTAGTGCACACCAGATACCCTGAACACATAGCCTCCAGTCCAT TTGCTGTGACGACTTCAGGGCCCCCAAAAGTCTCTCCAAAAAGAGGCATCCCTAAACCTCCCCCTCACCA TGCACTAGGAACTGCCCACATGGACACAGACTTACAGACACCCAAGACTTGTCCTTCAAGCTGTGAACTG CTAGAGGTGACATCTTGTAAAAGCCTGTCTCCAGGGAGAAAAAGACAGTTGAGTGACAGCACTGTGATGC CCCACAGGCCTTCCTTCTCAGGAGTGAATGAGTCCTTGACACCTCAGGTTAACAGTCCATCCTCCTCTTC CTCATCCTGTAGAAACCAAGGGTCCTCACAAGGTGGCCAGAACTCTCCCGAGAAAGGACCTAAAACTCGC CTCCCAGTAGGACTCAAAGTTCTCATGAAGTCTCCTCAGTTGCTCAAGAAAACTTCCTTGGTACCAGGGA AACAAGAAAAGGATAGTTTAAATGAAGCATCTCGAAGCTGTGTAGCAGCAAACACGTCTAATCCTGAGGA CTCCAAAGACCCTGCAAGTCTGGAGACCTCAAGAGATGAGAGAACCACAGCCTTACTAGGTGTGAGAGGT CCCGACAGTCTAGCTAGAGGACTTGCCTTAGACTCAACAATGCCAGAATCGTTAGAATGTAGCATCTCCA GAGATGATGGAAGAGATGGGGCAGACAGCAGAGCCATAAAAAGATCCTTCTCCTCCAGCAAGCCACACCT AAAGCCTGCTCTAGGCATGAATGGAGCCAAAGCTCGGAGTCAGAGCTTCAGCATCCACTTGGGAGAGAAG CCTTCCACACCCCCAACAGAAGGGCCAGGCAAGATTCGAACTCAGATTATCACCAACACGGCTGAAAGAG GCAACTCCCTCACCAGACAGAATATCTCAATGGAAGGGTCCCCAAGCAAGACCCCCTCTGTCTCAACATC TGATAATCTGCCCAATGCTGGAAGGCCCCTGGGAAACCCCTTTTCCAGGCAGAGTTCTCTCTGTAGCACC GGAAGTTCTAGCAGCCAGCACGGAAGCCCAAGCAAGTTACCCTTGAGGATGCTCACAAGGCCGGAAGGAC TCCTCACTCCTGGAGTGGAAGACCAACGGCTCTCTACTCAGGAAGAGTGTCCTGAAAGAGGTGTACATGA GCAATCTAGCAGTGACCACCATAGGCATCCATCTCCTCTTTCAGACTGCCCTCAGTTCCTGCACGTTCCA GGGAGGATGCAGCGCCCAAGCAATTTGGAAACAAGTGGGATATCTAAGCTAGAAACTTCTGGAAGGCATC CAGATGCTTCTTCAACTGGGACTAGTGTCGTGAGCCCAGAAGCCCCCCTCTCACCCTCCATCGAAGAAAA GGTCATGTTGTGCATTCAGGAGAATGTGGAAAAGGGCCAAGTGCAGACAAAGTCTGCCTCTGTGGAAGTG AAGCCCAGGGCAGGGCCTTCGTTCACCAGCTGGTTTGGTTTTCGAAAGAGCAGGCTTCCAGCTCTCAGTA GCAGGAGAATGGAAGCTTCCAAGAGTAAAGTGGAAAAGAAAGACACAAAACTGAAATCTGAGAGGAAAAA AGAAAAGAAGAAAGCTGAAGTACAATCAAAGATAGAAAACGAACTGAGTAGGGGGACCAAGAAGGCAGAT GGTCAGAGTCCAGACGATGGTCTGCAAAGCTCAGAGAGTCTGAAAGTATCCCAAGACATTTATAACCAAA TGAAGTTGGAGCCAAGGAATAGACCCAGCCCTGCTGTGTGCCCAACAAAAGATGCCTTCATGACAGAACT TCTAAACAGAGTTGATAAGAGAGCCTCGGTACAGAGAGAGAGTGGATCAAAGGGTGTTTCCTGCAGGAGC GTGTTAGAGGGCACCTCCCAGGGCTCCTGTTTCGCCAGCGGCTCTGTGAGCACTCAAGGAAGTCAAAAGA AAAACATCAAAACCAAAGTCGATATGGAAAAACCAAGAGAATCCTTGGGGGCCGAGGTCAATGAAGATGT ACAAGAAGATGAAGAAGATAGAGTTGCTGACACTACTCTCCAGAGCCACATGATAGAATCCAACTGCCAG ATGAGAACACTGGACAGCGGGATTGGAACCTTTCCCCTCCCAGACTCAGGAAACCGCTCAGTGGGACGAT ACATATGCCAGCAGGACTCTCCAGAGGACACTGATGCCCTCCTGCCTCTCCAGCCAGCCGCTTCCATGGC ATCCTCTGCCAGAGCCCAAACCCTTGACCGCGAAGTGCCTTCCTCCACAGACAGCCAGAGCTCCCCGGAG TCCATGATCATCCATTCCATATCTGACCCCAGCATGATGGCCAGAGAGATGAGAGCCTCACAGAGTCACC TTCCCAAGCCTACCTCCTCAGGAAAACCTAGTTTTGAAAAGCAAAATGGAGCAGAGTCAAAGGCTCACAC TGGCCCATCATTCGAATATGCTGAGGATGCAGTGGCAAGTGAGCCCCTTTCAGGATGGAGGGGTGAAGAT GCTACTACTGAGACCCAGGAACCGAAACAAGTTGAAGAAACAAAAGAAGATCCTGAGAATAGATTATCTG AAATTTCCCTGGAGTCATTCAATAAATTTAGCAGCAATACTGTGATTTTATTAGGAAAAGAGAAGTCTCC AAGTGAGGTTGAAGGACAAAAGGAAGAGAAAGGCAAAAAGACAGAAGTGTCTTCGAGTAGCTCCGAACGG CCTGGGGTGGACCGCGTGGAGTCTCTGAGTGACTCTCTGTATGACAGTTTCTCCTCTTGTGCAAGTCAAG CATCCAACGAGGCCTAGAGGAGCATCCTTCAGTGAGACTCCTGCATGCATGGTGCACTAGGCAAACAGAC TCCAGAGAAGCTGATAGCCAGTATACCAGAGTTTACTTCTCTCGTGGACTGATAGAGATCGCCTCGACTT ACTGTGTTTTGAGAGGATTAAAAAGCAAAATATGATGGAGAATTCTCAATGTTTGCACTTTTTTTGAGTA TTTGTATAAAAGCTGTACATTTTTTTGGGGGGGGAGAGAAATTATATTTGTAGGGGGAGAAAGATAGCAA TAAAAAGAGTTGGCGCCATGCTCTTGAAACGATGCATTAAGTTCTAGAAGTTGATAATATATTTTTTAAA AACCCCAGCTGAAGATTTTGTGAACATAATCATCTTGGTCCTCAACTCATTTGTGCATATACTGTCAATG GAAGCGTGGGGCAGGCAGGAAAGCCAGAGGGGTGGAGGTCACCTGTTCTGAAGGTCTCTCTCGATTGGGT GGTCCACTTGCCACATTGTCTCGAGGAAGATGTGATGATATGGGTGTTCTGTTGAAGAATTAGAGTGTGA GCACTGATTAGATGGCACCAGATGATGCGGAAGGGAGCAGATCTTAGCAGGCTTCACTCCCAACCTCTTC TATCCTCTCCATTGTATTTCTCACAATTCTGAGTGTTTGCTGTTTTCTTTTTAAATTTCCCTGCTTTTAT ATTATTATTAAAACCTGGGTACTCTTTGCCATATCTTAAAAATAAATGAATGGTTGGTGCTATGAAACTT TCTTACTTTTTAATATCCTTTTTTTCTATTAAAGACTGTTTATATGGGAGGATAAATTCTATTTTAAAGG TTATGTGTATTTTTTCTAGACGTGAACTATTTATAATTCCTTCTGTACAGAAGCCTACAGACCAGGCAGA CTAGAGCTATGTTTAGGATTTGTGTGGTTACCTTAGAACACTTGTATCTTTATAGAGTATTGTATGACCA GTGTTATTGTATTTGTGCAATTTGTTGTATTTGGATTTCATTACCTTAAATTCAAGCTGCGTGTAAAGTA TCCTCCTTGTCTTTAGAAAAAATTCTTTTGTTTCATTTCATTTTGGAATAAAGGTCTTATCACAATTACA CCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6856184 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6856184 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13049 same embryo
	EMAGE:13048 same embryo
	EMAGE:13046 same embryo
	EurExpress:euxassay_013783 same experiment
	MGI:4826621 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source