EMAGE mouse gene expression spatial database

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EMAGE:13053

Ccdc78 coiled-coil domain containing 78 ( MGI:2685784)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017251. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017251_01	euxassay_017251_02	euxassay_017251_03	euxassay_017251_04

euxassay_017251_05	euxassay_017251_06	euxassay_017251_07	euxassay_017251_08	euxassay_017251_09

euxassay_017251_10	euxassay_017251_11	euxassay_017251_12	euxassay_017251_13	euxassay_017251_14

euxassay_017251_15	euxassay_017251_16	euxassay_017251_17	euxassay_017251_18	euxassay_017251_19

euxassay_017251_20	euxassay_017251_21	euxassay_017251_22	euxassay_017251_23	euxassay_017251_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13053_wholemount_strong.wlz

13053_wholemount_moderate.wlz

13053_wholemount_weak.wlz

13053_wholemount_possible.wlz

13053_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13053_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37856
Entity Detected:	Ccdc78, coiled-coil domain containing 78 ( MGI:2685784)
Sequence:	sense strand is shown >T37856 AGACACATCTGGAGCTGAGTGAAGAACAGCGGTTGCAGATTTCTAAGGAGCTAGTTGATCTGCAGATTGC AACCCACCACCTGCGCGAACAGCATGAAGCTGAAGTCTTTGAGCTGAGGAGGGAAATTCTTCGACTGGAA AGTCGGGTGCTAGAGCTAGAACTGCATGGAAATGGTGCCTGCCAGGGACATAAAGTCCAACCAATGGCCA ATCTGGGGCAACACCAGGTGCCACCATTAGAGCCTCCAGGAGGCCAGCAGAAGCTGCAGGAGGAACTGAA ATGGCTTCTGGAGCATCACAGAGCCAGGCAGCAGGCACTGGAGACACAAGTGGGAGTCCTAAGCCAGCAG TTGCAGGGAGCCCGAGAGGAAGCCAGGACAACTGGTCAGCAACTGGCTTCCCAAGCCATGGTGCTGGCCT CCTGTAAGGGTCAGCTCCGGCAGGCTGAGGCTGAAAACACTCAGCTGCAGCTCCAGCTGAAGAAGATGAA CGAGGAGTATGCTGTCCGGCTGCAGCACTATGCCAGAGAGACGGTGGAGAACGCCAGTAGCACAAATCAG GCGGCCCTTCAAGCTTTCCTGGAGTCCACTCTGCAGGACATTAGGGCTGCACACCGAACCCGAGAGCAAC AGCTGGCCCAAGCTGCCCGGACCTACCGAAAGCGCCTGGCAGATCTGAACCAGAGGCAGGAGTTGCTGCT AACCACCTGCAGGGCTACCTTTGCAACAGCCATCAACCTGGAGCCATTACCCATGCACTGGGCCACTGAA CTCAGCCACCCAAGAGAAAAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 85286. Forward Primer - name:085286_F_cDNA_LOC381077, sequence:AGACACATCTGGAGCTGAGTGA; Reverse Primer - name:085286_N_SP6_cDNA_LOC381077, sequence:GTTTTCTCTTGGGTGGCTGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13051 same embryo
	EMAGE:13054 same embryo
	EMAGE:13050 same embryo
	EMAGE:13052 same embryo
	EurExpress:euxassay_017251 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source