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EMAGE:13069

Dffa DNA fragmentation factor, alpha subunit ( MGI:1196227)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13069 EMAGE:13069 EMAGE:13069 EMAGE:13069 EMAGE:13069
"Pseudo-wholemount" of euxassay_017284. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017284_01 euxassay_017284_02 euxassay_017284_03 euxassay_017284_04
EMAGE:13069 EMAGE:13069 EMAGE:13069 EMAGE:13069 EMAGE:13069
euxassay_017284_05 euxassay_017284_06 euxassay_017284_07 euxassay_017284_08 euxassay_017284_09
EMAGE:13069 EMAGE:13069 EMAGE:13069 EMAGE:13069 EMAGE:13069
euxassay_017284_10 euxassay_017284_11 euxassay_017284_12 euxassay_017284_13 euxassay_017284_14
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euxassay_017284_15 euxassay_017284_16 euxassay_017284_17 euxassay_017284_18 euxassay_017284_19
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euxassay_017284_20 euxassay_017284_21 euxassay_017284_22 euxassay_017284_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13069Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13069_wholemount_strong.wlz
13069_wholemount_moderate.wlz
13069_wholemount_weak.wlz
13069_wholemount_possible.wlz
13069_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13069_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30357
Entity Detected:Dffa, DNA fragmentation factor, alpha subunit ( MGI:1196227)
Sequence:sense strand is shown

>T30357
AACCGTGTCTGCTACGCCGCAACCACAGCCGCGATCAGCACGGCGTGGCAGCCTCCAGTCTCGAGGAGCT
GAGGAGCAAAGCCTGTGAACTCCTGGCCATTGATAAGTCCCTGACGCCGATCACCCTGGTCCTGGCTGAG
GACGGGACCATAGTGGATGACGATGACTACTTCCTCTGCCTTCCTTCCAATACGAAGTTTGTGGCGTTGG
CCTGCAATGAGAAGTGGATTTATAATGATTCCGATGGAGGGACGGCTTGGGTTTCCCAAGAGTCCTTTGA
GGCAGATGAGCCGGACAGCAGGGCAGGGGTGAAGTGGAAGAATGTGGCCAGGCAGCTGAAAGAAGATCTG
TCCAGCATCATCCTGCTGTCAGAAGAGGACCTCCAAGCGCTCATCGACATCCCATGTGCAGAGCTGGCTC
AGGAACTCTGCCAAAGCTGTGCCACTGTCCAGGGGCTGCAGAGCACACTCCAGCAGGTGCTTGACCAGAG
AGAGGAAGCCCGCCAGTCCAAGCAGCTCCTGGAACTTTACCTCCAGGCCTTGGAGAAAGAGGGCAACATC
TTGTCCAACCAGAAAGAGTCCAAAGCTGCCCTCAGTGAAGAGCTGGATGCAGTTGACACAGGCGTCGGCA
GAGAGATGGCTTCGGAAGTGCTGCTCAGAAGCCAGATCCTTACCACACTGAAGGAGAAGCCTGCCCCAGA
GCTGAGTTTATCTAGTCAGGATTTGGAGGTGGGCAAGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5367607), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 60244. Forward Primer - name:060244_F_IRAV100_d01_Dffa, sequence:AACCGTGTCTGCTACGCC; Reverse Primer - name:060244_R_SP6_IRAV100_d01_Dffa, sequence:TTCTTGCCCACCTCCAAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13067 same embryo
 EMAGE:13070 same embryo
 EMAGE:13068 same embryo
 EurExpress:euxassay_017284 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS