EMAGE mouse gene expression spatial database

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EMAGE:13071

Atf7ip activating transcription factor 7 interacting protein ( MGI:1858965)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017316. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017316_01	euxassay_017316_02	euxassay_017316_03	euxassay_017316_04

euxassay_017316_05	euxassay_017316_06	euxassay_017316_07	euxassay_017316_08	euxassay_017316_09

euxassay_017316_10	euxassay_017316_11	euxassay_017316_12	euxassay_017316_13	euxassay_017316_14

euxassay_017316_15	euxassay_017316_16	euxassay_017316_17	euxassay_017316_18	euxassay_017316_19

euxassay_017316_20	euxassay_017316_21	euxassay_017316_22	euxassay_017316_23	euxassay_017316_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13071_wholemount_strong.wlz

13071_wholemount_moderate.wlz

13071_wholemount_weak.wlz

13071_wholemount_possible.wlz

13071_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13071_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9242
Entity Detected:	Atf7ip, activating transcription factor 7 interacting protein ( MGI:1858965)
Sequence:	sense strand is shown >T9242 GCGGTGGCGGCGAGTGGAGCGCACGGCTGAGACGGCAGCGTCCGGGACGGCTGCACGGGACGGCTGCGTA GGAAGGAACTCGGGTCCCAGGCTCTCACCTCCCGCCCCCGAAGATTCAGAATGGATAGTGTAGAAGAACC TCAGAAAAAAGTCTTCAAGGCTCGGAAAACAATGAGAGCGAGCGATCGCCAGCAGCTCGATGCTGTGCAC AGAGTCAAGGGGGAGCTGCTGAGAGCCGACGGGAAGCTGCTGAACGGGAGCCATGAGAATGGAGACTTGG ATCCCACTTCACCCTTGGAAAACACAGATTGTATTCAAGATCGAGAAGAAGTGAATGGTATTGATGGGAT TTGTTTTCAGTCAGAAGAAAGTACAACGGAATGGAAAGAGACCCCTTGTATGCCCAATGTTGCTGTAAAA AACAAACAAGAAGATTTGAATAGTGAAGCTTTGTCCCCCAGTATAACTTGTGATCTTTCTTCTAGAGTAA CCACTGAACCAGGTTCTGGTAGTCCAGCCTCTGATAACCCAGGCTGTGGTACCCCAGTCTCTGATAACCC AGCCTCCGATAACCCAGCCTCTGACAACCCAGCCTCTGACAACCCAGACTCTGGTGATCTGGCTGCAGGA GAGCTGGCCACTACAGTACAGGCTACTGGTGACTCTGCCTGTGAGGAGCCTCCCTCCAGTGATCCCTCTT CCAGTGATCCCACTTCCAGTGAGCCCTCTTCCAGTGAGCCCACCTGTAGTGAGCCCATCTCTGGAGATCC TGTTTCTGAAGAAGCCGCCTCTCATGATCTTGTCTCTGGTGATTCCACTTGTAGCGAGCCAGTTTCTGGT GAGCCGGTGTCTCATGAAGCAGCTTCGAGTGAGCCAGCTACAAGTGAGCCAGCTTCAGATGAACCGGTCG CAAGGGTCGTGGCGGCCTGCGAACTGGCTCCTGGGGAGTCAGCCTTGGATGATTGTGCCCCCTCCGGTGA TTCACAGTCTGATGAGCCACCTTCTAGTGAGGACTCCCTTCCTAGGTCTGTCTGCAGCGGACTGGCTTCT GGTGAGCTGACTCCTGGGGAGCTCTCTGTGGAACCAGCCACCGACACTGTTAAACCAAGCTCATCGGCAG TTTGTGAAGCAGGTCCTGACCCTGACAAGACAGAGCCGAGTAGCAATAACAGTGATGATTGTCCAGGCAA GAGTGAAGACGACGAACACTTAGACCAAATTCAAAGTAAAGACTCCTGTGATGAGGGAAATAAAGTGAAC AGTAATGTCGTCGAGAAGGAAGAACCTCTCGAAACACACAGTGCAATTATTTGTTCTGATCTACCTCCAG AAAACACAACGAAGATAGCGGAGGATCCCATCGCTGAGCCTGCTCTCGAGGAAGAGGCCATCTCCAGCAG CATGGAAGTTGACCAAAGCGAAAAGGATGAGCATAAAAGTCCTGCAGAACCTGTAGCCGCTGTCAGTGAG GACCCCGCCGAGGAGGACAAGGAGGATACTGTCGTAGACAACACGGACTCCATGGAAACAGATGAGATCA TTCCGATTTTGGAAAAACTCGCACCCACCGAGGATGAACTCAGTTGCTTTTCAAAAGCTTCTCTCCTTCC AGTCGAAACAAGTCAAGATTTGGAGGATAAAATGGAGGGCTCTTTCGGCTCACCCTCGAAACAAGAAAGT AGTGAGAATTTGCCAAAAGAAGCCTTTTTAGTCCTTTCTGATGAAGAGGATCTTTCGTGTGGAAAAGATG AGTCCGAAGCTGTAGCGCAGAGTAAGATGAGCACCCCAGAGGGAGAAAAGAGTGAGAAGGACGGCAAAGC TGAGGAAGAGGAGCGAGTTCCAGCCGAGGAGCAGCCACCTGTGAGAAATGAGTTTTCTAGACGAAAACGT TCTAAGTCAGAAGACATGGACAGTGTAGAGTCCAAACGCCGGCGATATATGGATGAAGAATACGAGGCTG AGTTTCAAGTAAAGATCACAGCTAAAGGTGACATCAACCAGAAGCTTCAAAAGGTCATACAGTGGTTGCT ACAAGAAAAACTGTGTGCCCTACAGTGTGCTGTGTTTGATAAGACGTTGGCAGAATTAAAAACTCGAGTG GAGAAGATAGAGTGCAACAAGAGGCATAAGGCAGTGCTCACCGAGCTGCAGGCCAAGATAGCCAGGTTAA CCAAACGTTTTGGAGCAGCCAAAGATGATCTTAAGAAAAGACAAGAAAGCCCTCCAAACCCACCCATATC ACCAGGAAAGCCTGCCAATGACACCAACAGCAATAATAATATGACCTACAGAAATGCAGGCACAGTGCGA CAACTGCTGGAGTCCAAAAGAAATGTTAGCGAGGGTCCACCACCATCCTTTCAAACTCCTGTGAATACAG TGTCTTCAGCCAGTCACGCCACTTCTACAGCAGTTGTCAGTAGTCAGCCTAAACTGCAGACCTCAGCAAC CTCGGGCTCTCTGCCGGCGGCACCTCTTCTTCCTGCGCCCAGCACAGCCACCGTAGTTGCCACTACTCAG GTGCCTAGTGGGACCCCTCAGCCTACAATCTCTCTGCAGCCCTTGCCAGTGATCCTGCATGTGCCCGTTG CCGTGACCTCTCAGCCTCAGCTCCTGCAAAGTCATCCAGGGACTTTAGTGACCAATCAACCATCTGGCAA CGTGGAGTTCATATCTGTGCAAAGCCAACCTACAGTGAGTGGTCTTACCAAAAATCCAGTATCCTTGCCA CCCTTGCCAAACCCCACTAAACCAAACATTCCGTCAGTGCCCAGTCCTAGTAGTATTCAGAGGAACTCTT CAACCACCGCCGCACCGTTGGGAACAACCCTTGCTGTCCAGGCCGTTCCAACCGCACATTCTATTGTGCA GGCCACAAGGACTTCTCTACCCACAGTAGGCCCGTCAGGACTCTATAGCTCTTCAAGCAGTCGAGGCCCA ATACAGATGAAAATCCCAATATCCACTTTTAGCCCTCCATCTTCAGCAGAACAAAATAGCTCTGCCACCC CAAGAATTGTTACAGAAAACCAGACAAACAAAACAGTAGATTCTTCTATTAATAAGAAAGCAGCCGATAG CACATCACAGAGTGGAAAAGCTTCTAGCAGTGACTCAAGTGGTGTCATTGATCTCACGATGGACGATGAA GAGAGCGGAACTACACAAGACCCCAAGAAGATAAGTCCTCCTTCTTCATCAACTGTGAGCACTTCTCAGC CCATGTCTCGACCGTTGCAGCCCATTCTGCCAGCACCACCTCTTCAGCCATCAGGCGTGCCCACAAGTGG ACCATCTCAGGCAACTATCCATGTTCTGCCTACAGCTCCAACCACCGTGAATGTAACACATCGTCCAGTA ACTCAGGTGACCACAAGACTCCCTGTACCGAGAGCTCCTGCAAACCACCAAGTGGTTTATACAACTCTCC CAGCGCCAACCACTCAGGCTCCCCTACGAGGAACTGTTATGCAGGCTCCTGCCGTTCGCCAGGTCAATCC TCAAAATAGTGTTACAGTTCGAGTGCCTCAGACAACCACATATGTTGTAAACAATGGACTGACTCTAGGC TCAGCAGGGCCTCAGCTCACGGTGCATCACAGGCCCCCACAAGTGCACAATGAGCCCCCACGCCCTCTGC ATCCAGCGCCCTTACCAGAAGCCCCACAGCCCCAGCGTCTGCCCCCGGAAGCTGCCAGCACTTCTCTGCC TCAGAAACCACACTTGAAGTTAGCACGAGTTCAGAGTCAGAATGGCATTGTTCTGTCGTGGAGCGTCCTG GAAGTGGACCGAAGCTGTGCTACTGTTGACAGCTACCATCTCTATGCTTACCACGAGGAGCCCAGTGCCA CCGTGCCCTCACAGTGGAAGAAGATCGGGGAAGTCAAGGCCCTGCCCTTGCCCATGGCTTGCACTCTCAC GCAGTTTGTGTCTGGCAGCAAGTACTACTTTGCAGTGCGCGCCAAGGACATTTATGGCCGCTTTGGGCCT TTCTGTGACCCACAGTCCACGGACGTGATCTCTTCCTCCCAGAACAGTTAGACCTTGGAGTCGTTTAGCT TCTACTTTTGAAAGTTTCACATCTTGGGCTTGTTTTTAATCTTGTGCATGAAATCCACTGTAAAAATCCA CCTTGTGCAAGATCTCCCGGACATGTGACTGTATACACTACGTTTGTTTATAACCGGAAGCAAAATAAAT TCAGCCCATAAAGCAAGCCTTCCCTGAACAATTCAAACTTCGGGGTTTTGAAATGTATATTTATTCCTTG CTTTTTGGTTTTGAGTTTTGAAACTGGGGAATATGTATGGGTGTTTGTGTGTTTTCTTCTTTTGTTTTGA ATTTTTAAATTTCTAGTTGTGTGTGTATCACAGTGCGATCTCCTGTTTTCCTCTCAAATTTACCTCTCTT CCAGAAGGAAGTATATTGCTGAGCGAATCTGAGGTGCAGAGCAGGTGTGATTCTGCCTCCAGAGGAGGCT AGAAGGTCAAAGAATCTAAATAAAACCCGAAGGAAGGAAGGAAGGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6834550 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6834550 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13074 same embryo
	EMAGE:13072 same embryo
	EMAGE:13073 same embryo
	EurExpress:euxassay_017316 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source