EMAGE mouse gene expression spatial database

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EMAGE:13073

Rexo1 REX1, RNA exonuclease 1 homolog (S. cerevisiae) ( MGI:1914182)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017336. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017336_01	euxassay_017336_02	euxassay_017336_03	euxassay_017336_04

euxassay_017336_05	euxassay_017336_06	euxassay_017336_07	euxassay_017336_08	euxassay_017336_09

euxassay_017336_10	euxassay_017336_11	euxassay_017336_12	euxassay_017336_13	euxassay_017336_14

euxassay_017336_15	euxassay_017336_16	euxassay_017336_17	euxassay_017336_18	euxassay_017336_19

euxassay_017336_20	euxassay_017336_21	euxassay_017336_22	euxassay_017336_23	euxassay_017336_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13073_wholemount_strong.wlz

13073_wholemount_moderate.wlz

13073_wholemount_weak.wlz

13073_wholemount_possible.wlz

13073_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13073_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9247
Entity Detected:	Rexo1, REX1, RNA exonuclease 1 homolog (S. cerevisiae) ( MGI:1914182)
Sequence:	sense strand is shown >T9247 GGGGCAAGATGGCGGCGTCGGCGGGGTCCGAAAAGCGCGAGCGGAAGCCTCCGGGGCGAGTGTGAGGAGG GGTCCCGGGGTCCCGGGTATCTCCGGGCCGGGGCCCCGCTGCGGAACGGACCATGCTACGTTCTACCGGC TTCTTCCGCTCCATCGACTGCCCATACTGGGCGGGAGCTCCCGGGGGGCCTTGCCGGCGGCCCTACTGCC ACTTCCGGCACCGCGGAGCTCGGGGTCCCGGCGCTCCCGGTAGCGGCGGCGCAGCATCTCCTGCATCAGG GCTTGGCTATGACCCGTACAACCCAGAGCTGCCCCAGCCTCCTGTGCAGAGGGAGAATGGTGCTCTGGGT CAGGGTGATGGCATGCTGGAGCTGGAGCTGGTCAACCAGGCCATCGAGGCTGTGCGCGGCGAGGTGGAGC TGGAACAGAGGCGCTACCAGGAGCTCCTCGAGACTGCACGGGGGCACAGTGCTGGGCCTTCAGCCCTGGC GCCTTGCAGCCCTGCCACAAGCATAGATGACGATGACACCTTCTCATTGGCCCTAACTTACACGCCGGGT GGCCTGCTGAGCCCGGATGCTGCCTACCAGCCCACCCCACTGGCTGTCCCTGCCGAGCCAGGGCACAAGT ACTCACTGGCCCCTTCAGACAGGAGTCAGGGCAGAGCTGCAGGAGGTGCTGGTGCCCTGGAGTATGTCCC CAAGGCTGTGGGCCAGCCGCGCCGCTGTGGCCGCCCTGTGTCTGGTGGCAAGTATGTGGTGGATAGTTCA AAACCTTCAACAGATCTAGAGTATGACCCACTGTCCAACTACTCAGCCCGGCACCTTGGCAGGGCCAGTG CTAGGGATGAGAGGGCCACCAAGAGACCGCGGGGCTCCAGGGGTGCTGAGCCATACACTCCTGCCCTCAA GAAGCCCTGTGACCCCTTCGGTGGCTGTGAGGCCAGGTTCTCAGACTCAGAAGATGATGTGACCAGCCCC CCAAAGGCTGATGTTAGCAGCCCCAAGGCTGGAGCTGACCCTGAAAGCAAGGCACCTGGGAAGCCTGTCT CCAAGGAGGGCAGAGAGCATGAGGACGGTGGCCTGCGGGGCACCAAGGAGATGGCTGTGCAGTATGACGT GGAAGACCTTGGGCAGCCCCCCAAAGCACCAGACACAGTGTCTGTGGTCAAGCCTGGCTCCCCAGCCAGG GCCTCTCAAGATGCTAGGGTCCCCAAGGAAGGTAAAGCCAAAAAGAAGAAAAGTGGGACATCAGCCAGCC TCAGCCACAAGGACAAGGTTCGCAAGAAAGACAAAAAGAAAGAGAAAGACCCAGCCAGGCCCCGAGGGAA GGAAAAGGTGTGTACGGACAAGAAGAAGCTGCCAGCCAGTAACCCCAGGGGGAAGGCCCAGGGGCCTGAA GGGACCAAGAAAAAGCCATCCTCTGCGACCACAGTGGCCAGCTCAGGGAAAGGGGGGTCAGGCCGCCCCT CCAGCACGGGACCTCAGGACTCTGGGCCTGGTCCCCATGCACCACTGGCCTGGAAGGCTGGTAGTGCAAA GAAGATGTCATCGGGAAAGTTGGTGGAACGCAAGGCCCGCTCCCTGGATGAAGGAGCCCCCCAGGACACC CCTAAGCTCAAGAAACGGGCCCTGAGCCATGCTGAACTATTTGGGGATGAGAGTGAGGAGGAGGACTCAA GCCTGGGAGCGGGAGCACCCCGGGTCTGGCCCCCGACCCTGCCCAGCCTTAGCTCAGACTCAGAATCAGA CTCCGACTCCAGCCTGGGCCTGGACGAGACAAAGGTGCCCAAGCGTCTCAAGGCCGCGCCGCCCGCATCC CCCGTGCCGCCCTCCCCATTGTCCTCCTCCTCCTCGTCCTCGGGAGCCAGCCAGTGTGCAGAGGAGGATG TGGACTACTCGGCTCTGGAGAAGGAGGTGGACTTCGATGTGGACCCTATGGAGGAGTGCCTGCGGATCTT CAACGAGTCCACCAGCGTGAAGACAGAGGACAAGGGCCGGCTGGCCCGGCAGCCACCCAAGGAGAAGGCT GAGGAGAAGACACATGCGGGCCTGACCACTTTGTTCCCGGGGCAGAAGAGGAGGGTCTCACACCTTTGCA AGCCAGGCAAGGAGTCAGAGGCCCCAAAGAGGACCCCCGTGGCTCCCCCAGCACGCCCCCCGACTGCTCA GGAGGTGTGCTACCGGCGAGCACAGCTAGCACAGAGGGACTCGGCCAGTTGGCTACAGGCCGCCCCGCGG CCAACAGAGAGGCTCTCCTCCGTGCACATCTCAGCGCCTGGGGAGAAGAGGAGGATTGCTCACGTACCCA ACCCCCGACTGGCTGCCGCCCCCACAGGTGCCAAGAGGGCGCTCTCTGCCAGCAGCAGCCAGGCGTCCCA CGGCCCCGAACCTGGCAGCCAGCCACTGAAGACACGCACATTGTCAGGGATGGCATCTAAGACAACCACC ACCGTCACCCCCAAGCGCATCGCCCACAGCCCATCACTCCAGAGTCTAAAGAAGCCTGTTATCCCAAAAG AATTTGGCGGCAAGGTTCCCACTGTGGTCCGCCAGCGCTATCTCAACCTGTTCATTGAGGAGTGTCTCAA GTTCTGTTCGTCCAACCAGGAGGCCATCGAGAAGGCCCTGAATGAGGAAAAGGTGGCGTATGACCGCAGT CCCAGCAAGAACATATACCTGAACGTGGCCGTGAACACCCTTAAGAAGCTAAGGGGCCTGGTCCCCAACA CCGTGCCCAACCTCAGCAAAGCCAGTGGCCGCAGGGTTGTGTCTCATGAGGTTGTACTGGGGGGCAAATT GGCAGCCAAAACCAGCTTCTCATTGAGCCGCCCCAGCAGTCCGCGGGTAGAGGAGCTGAAAGGGACTGCC CTGTACAGCCGCCTCAGGGAGTACCTGCTCACTCAGGAGCAGCTGAAGGAGAATGGCTACCCCTTCCCCC ACCCAGAGCGCCCAGGGGGTGCCATCATCTTCACAGCCGAGGAGAAGAAACCCAAGGACCCCTCCTGCAG AATTTGCTGTCGCTGTGGCACAGAGTACCTTGTGTCCTCCTCGGGCCGCTGCGTGCGCAGTGAGGAGTGT TACTACCACTGGGGGCGGCTGCGGCGGAATCGAGTGGCTGGGGGCTGGGAGACCCAGTATATGTGCTGCT CAGCTGCCGTCGGCTCTGTAGGCTGTCAGGTTGCCAAGCAACACGTGCAGGACGGCCGGAAGGAGAACCT GGAGGGCTTTGTGCGCACCTTCCAGAAGGAGCTGCCTGAAGATGCCCATGCAGGGGTCTTTGCCCTCGAC TGTGAGATGTCCTACACTACATATGGCCTGGAGCTGACCCGAGTCACAGTTGTGGACACAGACATGCAGG TCGTGTATGACACCTTTGTCAAGCCTGACAATGAAGTTGTTGACTACAACACCAGGTTCTCAGGGGTGAC AGAGGCTGACCTTGTGGACACGAGCATCACGCTGCGGGACGTGCAGGCTGTCTTGCTGAGCATGTTCAGC GCCGACACCATCCTCATCGGACACAGCCTGGAGAGCGACCTGCTGGCTCTCAAGGTCATCCACGGCACAG TGGTGGACACGTCTGTGCTTTTCCCACATCGCCTGGGCCTGCCCTATAAGCGCTCCCTGCGGAACCTCAT GGCTGACTACCTCAGACAGATCATCCAAGACAATGTGGACGGGCACAGCTCCAGTGAGGATGCCAGTGCC TGCATGCACCTTGTCATCTGGAAGATCCGAGAAGATGCCAAGACCAAGCGATGACTCCCACCCAACATTA CCATGTCACCCCCCTTCTTCCATCCCTGGCTGCCCTCAGCCCCAATCCCTCAGCCCCGATGCCCTCGGCC TCTTTTCAAACTCTGTAATAAAACATGACAGCTGACCACGCGTCTCCCAAGCTCCAGCTGCAGGTCTGCA GTGAGCAGCCCAGAGTCGGGGCTGAGACCAACCCTCAGCCCAACCCCACACCTGCCCAACGTGACATCAC CTCCTTTCCCAGTCCTGTCCCCTGGAGCCTGCTGCAGCCCAGGGCAGAGCTGGCCTAGCCTCTGTGCCCC AGTCAGGCACCCCCACTCTTACCCCAGCCCACTGCTCTAGACAGTGGGCTCTGCCTTCTGCTTCTTTGTA TATAACGCAGGCAACAGGACAAGCCCTCATGGTTTGAGACAGGGGTTGTCTTTTTTTACTTTATACTGTT ATTTTTATTAATTTTTAATTTAAACCTGCTGACCCGCACACCTCCTCAGCTGAGGGGTCCAACCCTGGTA CCGCTGGGATCCAGGGACCGAACCCCACATGGTCAGTCCCATGGGCCTGGATGTTTGCCCTGCCTTGTGG GGTAGCTTCCAGAACTCACCCTAGCCCACCAGGGCTCCCCAACCTCAGAGCCAAGCCAGCCCTTTGGCCT GGCCTTCCAGGGCCTCTGGGCTGTCATCCTGGGGGCTATTGCCTCCCTCTGTGAGTAGAAACAGAATAAA TGTTTGTATGAACTTTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6835200 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6835200 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13071 same embryo
	EMAGE:13074 same embryo
	EMAGE:13072 same embryo
	EurExpress:euxassay_017336 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source