EMAGE mouse gene expression spatial database

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EMAGE:13076

Fam168a family with sequence similarity 168, member A ( MGI:2442372)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017235. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017235_01	euxassay_017235_02	euxassay_017235_03	euxassay_017235_04

euxassay_017235_05	euxassay_017235_06	euxassay_017235_07	euxassay_017235_08	euxassay_017235_09

euxassay_017235_10	euxassay_017235_11	euxassay_017235_12	euxassay_017235_13	euxassay_017235_14

euxassay_017235_15	euxassay_017235_16	euxassay_017235_17	euxassay_017235_18	euxassay_017235_19

euxassay_017235_20	euxassay_017235_21	euxassay_017235_22	euxassay_017235_23	euxassay_017235_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13076_wholemount_strong.wlz

13076_wholemount_moderate.wlz

13076_wholemount_weak.wlz

13076_wholemount_possible.wlz

13076_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13076_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9390
Entity Detected:	Fam168a, family with sequence similarity 168, member A ( MGI:2442372)
Sequence:	sense strand is shown >T9390 GGCTACTGCAGAGGATTCAGATCTGAGTAGCTGAGGAGAAGGAGGAGGAGGCTCCCGTCCCTGCGCGCCA TCCACCACCCTACTGGACACGGACGAGGGAACGAGAGCGTCGCCGCTGTAGCCTCCAGAGAAGACAAGGG CATCGCCGCTTCAGTTGCGGCCGCCGCGGTTTTCGTCTGCAGCAGCTCGCCGGGCTGGCTTCCTGGCTCA CTAGACTGACTTCCTTGATTTTGATCCTCAGTCTTCCACAATGAACCCTGTTTATAGCCCCGTGCAACCT GGGGCTCCTTATGGCAACCCTAAGAACATGGCCTACACTGGTTACCCCACAGCATACCCGGCAGCTGCTC CTGCCTACAACCCCAGCCTGTACCCGACCAACAGCCCTAGTTATGCTCCAGCCACTCTGCTGATGAAACA AGCCTGGCCACAGAACTCGTCTTCCTGTGGCACTGAAGGCACCTTCCACCTCCCAGTGGACACCGGGACC GAGAACCGAACTTACCAGGCATCCTCTGCGGCTTTCAGATATACCGCAGGGACACCATACAAGGTCCCAC CGACCCAGAGTAATACTGCTCCACCCCCCTACTCCCCATCACCCAATCCCTATCAGACGGCCATGTATCC AATCAGAAGTGCCTATCCCCAGCAGAATCTGTATGCCCAGGGAGCCTACTACACACAACCTGTGTATGCT GCCCAGCCCCATGTCATCCACCACACCACGGTTGTCCAGCCCAACAGCATTCCCTCTGCCATCTACCCAG CTCCTGTTGCTGCCCCCAGGACCAATGGTGTGGCCATGGGCATGGTGGCAGGCACCACCATGGCAATGTC AGCAGGTACCCTGCTGACCACACCCCAGCACACTGCAATTGGGGCACACCCTGTCTCCATGCCCACATAC AGGGCCCAAGGAACCCCTGCGTACAGCTACGTGCCCCCGCACTGGTAAAGCCTGCAGCCCATCCAAATCT GGAGTCACATTGTATGCAACTACTCGAGTCTTACCGGTGCTGAGCAGTCTCCTAGCAGCACCACCTCTAT TGCAAGAAGAGACAACGGAAGTGCAAGGGTCACTTTATGCATCTTTAATGGTTTTGATTTTTATTTTTGG TTTTTGTAAAAGCTGCTTTTTCTAATCTCCTAGGAGCCTCCCCTCGTGCCTCCCTCTTAGCCACACCCTT CTGCTCTAGCCCTTCTTCCTACCTAGCCAGGCCCAGTCCTCTCTGCTTCTTCCCTTCCCTCAGCATCCTG TCCCTGGGACCCCGGTCTAGTAGCAAGCAGAGAGTGAGGTTTATTGTGATGGTTCATCTAAAGGCTAGAT TCGTTTGGAGAGCAGAACAAATGTGGCCCTCCGTGTGGAGTAGGGACTTGAAGCTGTCGAGAAGGCCACA TCTCTGTCAGATTGTCATTTTCTGAAGCAAGATTAGTTCAAAAAGAAAGAAATATCTAAGAACCATCACA ACAGACCAAGAACAAACCCACCTGGTTATGTAGGAATGTTAGCAGCTTCATGTCCCCAGGCTCAGAACCT GGGAAAGCAGAGAGTTTGGGGAAGGGTGGAGGGAGGGAAGACACTGGGGAGGGAGGTAGTGTGAAGAAGG GCAGGAGGGTGCTTAGCTCTGGTTTGAAAGGTCTCCGGCCCCACTGCTGGGCCTGGACCTTCTGATTTGC ACAGTGATGTTAACTGACCTGGCACTTCCTTCAGAGATGCTTTGACCTCAGACCTGCCAAACAGCTGCAT GTGCTGGGATACCAGCATCCAGAGGCTTCGCATAATCCCACACCCAGCCTGAGCCTCGAGGACCTCTCCT GAGGCGCTTCCTCCTGATGAGAGTAGGAACTGGTGGCATTTCTCTCCCTTGCCCTTCTGCCCTTCCCCGT GCCGGACGCACACGTCTGTCTGTGGGGCTGGCAGGCAGGCCAAGCTCTTGGTTACCTTGTGCCATGTCAC GGCCAAAGTGAAGGAACCACATTCCAGGTGGGAGCTGGCGGTTCTGAAGGTCAGGATAGTGAAGGAAAAC AATAGCAATAATCATTTCATACTCATGGAACTGAAGGAACCTTAGCATCCATCATCTCACCCAGTCCCCT ATCTGACAGATGAGGAAACTGAGGCCCAGAGAAGCAGAAAGGACTTCTCTGAACCTCACTGTAAATTAGC ATTAAATGCAGCCGTCCTACTACTGGCATTTTACCTACAAAAATTTTGCTAGGGATGGATTGGCTTGACT TTGTAAGAATTAAGTCAGCAGTGCCACATCTGGTGAGCTCTGTAATGACCTCTGGGCTTGTTTCTGCTGT TCCCCCACGGACTCCAAAGGTGGGGGCTAAGAGAGGCCACATAGAGCAGGCGGCAGAAATAGAAATTACA CTTGGAAAACCTATGAAGACATCTGGAATGTTCATCTTGAACCTGACTCCACTCCAGGAAAAGGAGTAGG TGGGCTGACTGGAAGGAGAGCCTGTGCACTTAGGACAGTAGCTCTTTATCCCAAGAAGAGAGGAAGAGTG GGAGCCACCCAGAGAGGTGCACATGAATGAGGCCAAGCAGATACAGTCACATCTGGGAGAAGAGGGACCC CGACCAGCCACCCTCCTGTGCACAGCTAGCAAAGCCGACATCTTCCCTGGGTGGAACAGGAATACCTTAC TGTGTAAAATTGGATCACTGGTAATATTTCAGACTTGAATTATTTTCATTTTAAATTCATTTTAAACTTG GACATCACCTTCCTATTTCCCAAGTCCCCCTAAAGGAAAAAAAAAGGAAACCACTGAGCTGGAATGACCT CAGAAACAGTCTGACTGTCCCCAGGGGGTCAGAGTTCTTCAGCGTCTGTGGCAAAGAGCCAGAAGTTAAG GCTGCTACCTTCCTGGTCTCCCAGGAACTTCAGGACCAGGAAAACTCAGCTGTTCTGGGGTGCTGACAAG GTTTCATTTTAAAACAAAGTTTTCCAGTGTTAATCTGATCTTATAACACCTCTCCCTGTCCTCTGTGGTT TTGTAGAGGTGGATGAAGGTTGCTAGAGATCATGTCTAACATGTTAAAAAATGCCAAACTGATAAAGGTG GTGGTAATTGTAGGTTACATTTGTTAGGTGCTTCATTAGTTTCAAAGCACTTCATGACCTTTAATATACT GACCCTGAGAGCAAGCAACAGGGTCGGTTATTCCACTCACTTGACAAGTGATGCGATAGATTCTAAGGAG AAATGAAATGGCCCAAAGTGAGCTCACTGCTGGGATCCTGGCAGGCTACCTAGACCCCACGGTCTCCAGG TGACCTGGTGGCAGAGGAAAACACAGACCCAGGAGTGGCCTTTTCATGTTTGTTCTTTCTACACCAGTAA ATAAAAGCTGTTTGCACCCCTCACTGTTCATGTGGGTTAGCCCTGACAGATGAAGGCATGGAGCCTGCTA TTTAATTCATAGAGGTTCCAAGGCTCCGAAAAGTACAGGGACTTTCTGGTAAGGAACCCCCTGCTGGCGC TGTTCTCTCCAGGCCTGATCCAAACATAGGCTCATGTTCTGAAGCATCTGTGCTCTGCACCTCATCTGGG ATTCAAGAGCAGAAAAGTCCCAAGGTGAAAACCAAGTATTGGCATGCTGTAGTCTAGAAGAACCAGACCC ATTAACTCTTTTTAGTAAGAGCACAAAAGCTGGCCTCACCCTTTTGTTTGATAGAGATCCACAAGTCTAG CCAAGCTTAGGGAGTCATAGTGAAGGGACTGAGCCCTAGGGCACAGGGGGTTATGTGACCACAGCTGGCA AGGTGACACGTAGCTGGCAGGAATACTCTAGGTATCATCTAAACAGTCTGGAGGCTCAGTGGTCAAGACA GAGGAGGCCAGAGCTGGAAGGGTCCCCTCACCATAGGATGAGGGGTGTGCACATGACCATGAGCTGCCAC ACTGGCATCACCATACACAGACACCTTGTCCACACCTTTTCCCCTCCCCAGTGCAGTGCTGAAGGAGAGG TGCCTGAAGGAGAGGTGCCTCTGTTCACCAGCCTCCCTTATCACCATTTGTTCACCTTTATCCACCCTGG GCCAGGAAGTCTGGAAACTGGACTACTCACAGTTAGTGAAAGCCTGAGAGTCTCTTGTCATGCTTAAAAC AAAACAAAACAAAAAACAAACAAAAGCCCTATTTTTCTTCCTATAAGATAAACCACGTTTTCCTTCTCGT TATCCCAGGAATGCCCTAAAGTGCACCCCCTTCCCTGAATATTCATCAGGGACACACATCTATCTGTCCA GACCTCTTTTTGTAAAGTACTTTGACTTTGATTGCCAAAACCTCTAAAGATAGGAGGCAGGGTATGCATC GTGATGCTATGAAGGGGTCACAGGCTTGCCAAAGGACACATAGCTCATGAGTGAAAGAGCCAAGATTTAG ACTCTTCTGATCCTCTATTCTTGTTGCTGCCTCCTACTGGATCTCGAGTGTAAGCTTGCTTGAAGAAAGA AGGGGTTACTTGCTACCCCACACAGAAGCGTTTATGAAGACTTGGGGAGCTCCGGTCCATCAGCTGAGCA GATTTTTAAGAATTTCTGCTTTGGGCCAAGACCTATAATGGGCCCTGGAGACCCAGAGATAGATAGATCC AGCTGGTAGTACAGATAGGAATTTGCCACAAAAGACCTAACTCAGGACACTGAAGGTGACAACTGCCTAG TACTGAAGATAATTGAGGACAGTATCGTCCATCTTTCAACAAAGAGTCTTGCTTCTGGAAAGCCTGCACA ATCTGTCCATCTGGCTCCTGTCCAGATCCAACAAAGTACGTTAACGGGGCATGGTATAAGCCTGAACTAC TGAAAGCATGTCTGGACTGGAGTACAAGGTTTATTTGAGAACCAGAAACTAACTTGTGTCCCATGTATCC CAGTGCTTTCTGGCCATCAGGGCAGTTCTACGTTTGAGACCTAGCAGTGTTTCTTTGGAAGGCTTGATGC TCTGTTCTAAATAGCTTTCAGAAATGAGTCATGGAGAGCATACAAGTAGCACAAATCCCTTGGTCACCAA GTTCTGGCATCTGTATTGTGCAGACATGACACATCAGATAAGGCATTGGCTGAACCAGGCTTAGTTCCCT GCATATCAGATATCTTGCCCCTGCTATGGGCTCTGGTCTTCACAGCGTTCTGGCAGAATCAGTCATCATC CCATGTGTGACTCATGTTCAGTGAGCTCTGCTTTTCCATCTTGAGTTAACTACATGACTGCTGAGTGTGT GGCCCCAAGTATCTTGTCAAGCTAGGGAGACTAGAGAAGGAGGGCAGCCTGGGTTGGAGTGGACAAAGGA GAGCAAAGCAGCGGAAACATCTTGGCTAGGTTTCCTGCTCAGACAACACCTGAGCCTCTAGACAGACTTC TGTGGACATGCCGGGCTGGCAGCAAGGTTTGTCCTTTTCCTACCCCTGTTCGCTCTCCTAGGGGGAAAAA AAGAAAAAAAAAAATTGTGAAATTTGGCATTATGTGGTTCCAGGGCTCAGCTTTCAAAATTCCCAGTAGG CCAGCTTCAACATTCCATGGCCAAACTCGCCTGACCTAGACTTGGGTCCCACTCTCAGTTTCTCCTGTGG GTATTTCCTAATTTATAAACTGTTAATGCCAGCCAGAAGGCCAGCTAAGAATATAGGAGTTGAAACTAGT GGCCACAGAGTCAAAGAGGGACACAGAAGACAAGCAGCACTTGCTTTCCTTACCGTGGCTGCTGCTTTCG GTAGCCCTTAGGTAGCCCTGCGGTGGCAGTGTGTCCCTTTATCCTTTCCAGCCTGAGGGACAATTTTCTT TCCCAAGAGAACAGATTTAAAACAGCTTTACAGATAGCAGCAGCCCACAGGAATCTGCTTTGGGTTTGAC CCTAGCATCCTCTGTCCTAGACCCCAGCTCAGCCATCCTCCTCATGCATGAAAGTCAGACTAAATGAAGA ATAGGACTGGCCCAGAGACCTCAGGTCAGCCAGATCAACTGGATCTGTCTTGTGAACCAAGCTCCTTACA AGGTGATGCAAGGCTAGGCCTCCTGCACCTTGGAGTTGTCTTTTATCTTTGAAGGCCCAGGGCCTGTCCC CTTCCTGTCTTCAAGGCATCTGCACCCCTCTTTCATAGCACATGACAGAGAAGCTTGTTCTCATGGCATG GACCATCCCTGTGAAGAGAGCGTTGTGTATGATTTTTGTATTTTTTAATTCATTTTAACCTACAGGTTGG AATCTAATTTTTAAATTTTATTGGAACTCACATTTTAAAACAAATAAAAAACATTTAAAAATAATAATAA TTATAATAATAAACCTTTGACCAGTGTCTTCCAAGTAGTTTGATCAAATAATTTATAGATGTTTTCAAAA TACAGCCTGGAGTGTAAAGATTGGAAAGCCCTGTGGCCACGCTTGTGTGTATGAAGTGTAAATCTGGCTT TGTTGTTTTGGATTCCTTTGGGTTTTGCTTTGTTTTGAAGATCAGATAGTGATCACTCTGAATGGTTGAA GCCAAGAGTTTGTAACTATGATATTTACTGTAAGCTGTAGAACATTCAACAACTATTAAAAGAAAGTTTC CAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6855858 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6855858 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13078 same embryo
	EMAGE:13077 same embryo
	EurExpress:euxassay_017235 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source