EMAGE mouse gene expression spatial database

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EMAGE:13077

Matr3 matrin 3 ( MGI:1298379)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017297. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017297_01	euxassay_017297_02	euxassay_017297_03	euxassay_017297_04

euxassay_017297_05	euxassay_017297_06	euxassay_017297_07	euxassay_017297_08	euxassay_017297_09

euxassay_017297_10	euxassay_017297_11	euxassay_017297_12	euxassay_017297_13	euxassay_017297_14

euxassay_017297_15	euxassay_017297_16	euxassay_017297_17	euxassay_017297_18	euxassay_017297_19

euxassay_017297_20	euxassay_017297_21	euxassay_017297_22	euxassay_017297_23	euxassay_017297_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13077_wholemount_strong.wlz

13077_wholemount_moderate.wlz

13077_wholemount_weak.wlz

13077_wholemount_possible.wlz

13077_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13077_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9378
Entity Detected:	Matr3, matrin 3 ( MGI:1298379)
Sequence:	sense strand is shown >T9378 GCTTTCTAGCTTGAGCTTTCTTTTTGGCCATCTTTTAAAACTTTTTTTTTTTTTTTTGATCTATAAAATA GACAAGAGATAGTTCTACAATGTCCAAGTCATTCCAGCAGTCATCTCTCGGTAGGGATTCACAGGGTCAT GGGCGTGACCTGTCTGCAGCAGGAATAGGCCTTCTTGCTGCTGCTACCCAGTCTTTAAGTATGCCAGCAT CTCTTGGAAGGATGAACCAGGGTACTGCACGCCTTGCTAGTTTAATGAATCTTGGAATGAGTTCTTCATT GAATCAACAAGGAGCTCATAGTGCACTGTCTTCTGCTAGTACTTCTTCCCATAATTTGCAGTCTATATTT AACATTGGAAGTAGAGGTCCACTCCCTTTGTCTTCTCAACACCGTGGAGATACAGACCAGGCCAGTAATA TTTTGGCCAGCTTTGGTCTGTCTGCTAGAGACTTAGATGAACTGAGTCGCTATCCAGAGGACAAGATTAC TCCTGAGAACTTGCCCCAAATTCTTCTACAGCTTAAAAGGAGGAGAACTGAAGAAGGCCCTACATTGAGT TATGGTAGAGATGGCAGATCTGCTACACGGGAGCCACCATACAGAGTACCTAGGGATGATTGGGAAGAAA AAAGGCACTTTAGAAGAGATAGTTTTGATGATCGTGGTCCTAGTCTCAACCCAGTGCTTGATTATGACCA TGGAAGTCGTTCTCAAGAATCTGGTTATTATGACAGAATGGATTATGAAGATGACAGATTAAGAGATGGA GAAAGGTGTAGGGATGATTCTTTTTTTGGTGAGACCTCGCATAACTATCATAAATTTGACAGTGAGTATG AGAGAATGGGACGTGGTCCTGGCCCCTTACAAGAGAGATCTCTCTTTGAGAAAAAGAGGGGCGCTCCTCC AAGTAGCAATATTGAAGACTTCCATGGACTCTTACCGAAGGGTTATCCCCATCTGTGCTCTATATGTGAT TTGCCAGTTCATTCTAATAAGGAGTGGAGTCAACATATCAATGGAGCAAGTCACAGTCGTCGATGCCAGC TTCTTCTTGAAATCTACCCAGAATGGAATCCTGACAATGATACTGGACACACAATGGGTGATCCATTCAT GCTGCAGCAGTCTACAAACCCAGCACCAGGGATTCTGGGACCGCCGCCTCCTTCATTTCATCTTGGAGGA CCAGCAGTTGGACCAAGAGGAAATCTGGGTGCTGGTAATGGAAACCTACAAGGACCAAGACACATGCAAA AAGGCAGAGTGGAAACCAGCCGAGTTGTTCACATTATGGATTTTCAGCGAGGAAAAAACTTGAGATACCA GCTGTTACAACTGGTAGAACCATTTGGAGTCATTTCAAATCATCTGATTTTAAATAAAATTAATGAGGCA TTTATTGAAATGGCCACCACAGAAGATGCTCAGGCTGCTGTGGATTATTATACAACTACACCAGCATTAG TGTTTGGCAAGCCAGTGAGAGTTCATTTATCCCAGAAGTATAAGAGAATAAAGAAACCTGAAGGAAAACC AGATCAGAAGTTTGATCAAAAGCAAGAACTTGGTCGTGTGATACATCTCAGCAATTTACCTCATTCTGGC TATTCTGACAGTGCCGTCCTCAAGCTTGCTGAGCCTTATGGGAAAATAAAGAATTATATCTTAATGAGGA TGAAAAGTCAGGCTTTTATTGAGATGGAAACCAGAGAAGATGCAATGGCAATGGTTGACCACTGTTTAAA GAAGGCACTTTGGTTTCAGGGGAGATGTGTTAAAGTTGATCTGTCTGAGAAATACAAAAAACTGGTGCTG CGGATTCCTAACAGAGGCATTGACTTACTGAAAAAAGATAAATCCCGGAAAAGATCCTATTCTCCAGATG GAAAAGAATCTCCAAGTGATAAGAAGTCCAAAACTGATGCTCAGAAGACTGAGAGTCCAGCTGAGGGTAA AGAACAAGAAGAGAAGTCGGGTGAGGATGGTGAGAAGGACACCAAGGATGACCAGACAGAACAGGAGCCC AGCATGCTTCTTGAATCTGAAGATGAACTGCTTGTTGATGAGGAAGAAGCTGCAGCACTACTAGAAAGTG GTAGTTCAGTGGGCGATGAGACCGATCTTGCTAATTTAGGGGATGTATCTTCTGATGGGAAAAAGGAGCC TTCAGATAAAGCTGTGAAAAAAGATCCAAGTGCTTCAGCAACATCCAAGAAAAAACTTAAAAAAGTGGAC AAGATTGAGGAACTTGATCAAGAAAATGAAGCTGCATTGGAAAATGGGATTAAAAATGAGGAAAATACAG AACCGGGTGCCGAATCTGCTGAGAATGCTGATGATCCAAACAAAGACACAAGTGAAAATGCAGATGGCCA AAATGATGAGAATAAGGAGGATTATACAATTCCCGATGAGTATAGAATCGGACCATATCAGCCTAATGTT CCTGTTGGTATAGACTATGTGATACCTAAAACAGGGTTTTACTGTAAGCTGTGTTCACTCTTTTATACAA ATGAAGAAGTTGCAAAGAATACTCATTGCAGCAGCCTTCCTCATTATCAGAAATTAAAGAAATTTCTGAA TAAATTGGCAGAAGAACGGAGGCAGAAGAAGGAAACTTAAGATGTACAAGAAGCTTAATTCAAAGAAAAC GATGGTTCTTTGATTTTAATGTTAACCTTTTTTAAAATACAATACTGATAGTTAGAAAACTACTGTACAC TTTTGTTTTAGTGGAAAATAATAGATGTCTGTTGTGTTTAAGTGTTATAGCAAAAAGTACACATACGGTT AAGTTAATGAAAATGTTTTTGTTTCATCCGAGTGACAACAGACAAAAGTACTTCCTAAAGATTGACTGTT GTAAGCTATGGAAAACAACTTGCACCACTAAGAGAAAGATAACATAGAACCATTCAGAAAAATGAAACTT AGTAGTTTGAGGCTTCAAAGAAATGTCAACTTCTGATTCCATTCAATAAAGAACAAAACCAATTGTATTT TTTTTTACTTTTCATCTGAAACATTCCACATTTTAATCTGAGCCTTGCAGACTTTTCATTTGACTTTGGA AGCTGTTTTGGTTGCATTGCATTTTTAGAGAACTTCATTCATGTGAGATTGGCCATTCAATTGCAGGTGC AGTTTTCTTTTAATGTCATGCTGTTTAGATGATAAGAAATATTAAGAATTGGCTTTAGATTTGTAATTTT TTTCTCTTGAGTTCCTGCTAGGTTTTATATTCTAGTAGTCAATGTTATTTCAGTGAAATGTAAAAATATT CCCGTTCTCTTTGACCAGTATTAATTTCTTGAGAACCTATTGCCTGTCACTTGAATCCTGTAGCTGTCAT ACATCTCTGGTATAAGCAACATTTGATTTTTGAAGTGTGTAAACCATCTATATTTTCAAGATGTAATTTT ACATTTCTGCATTTCAAAACCAGTTTGGCCATAATTCTTGACGCACGCTTCTAATTCATGTACCTGCACA TGTGACCTTTGTGAGCAGAAATTTGCATGTATAATCTGTGTTTACTTGTAACTCTCTGGTTATATACTGC TTATATCTGTGGATTCAAGTTACTGAAGTGAATACCAATAAAAAAAAAAAAAACTTAGGTCATGTTGATT GGTTGGTTAAACATGTTTGGATGTTAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6854095 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6854095 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13076 same embryo
	EMAGE:13078 same embryo
	EurExpress:euxassay_017297 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source