EMAGE mouse gene expression spatial database

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EMAGE:13078

Dhx37 DEAH (Asp-Glu-Ala-His) box polypeptide 37 ( MGI:3028576)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017285. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017285_01	euxassay_017285_02	euxassay_017285_03	euxassay_017285_04

euxassay_017285_05	euxassay_017285_06	euxassay_017285_07	euxassay_017285_08	euxassay_017285_09

euxassay_017285_10	euxassay_017285_11	euxassay_017285_12	euxassay_017285_13	euxassay_017285_14

euxassay_017285_15	euxassay_017285_16	euxassay_017285_17	euxassay_017285_18	euxassay_017285_19

euxassay_017285_20	euxassay_017285_21	euxassay_017285_22	euxassay_017285_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13078_wholemount_strong.wlz

13078_wholemount_moderate.wlz

13078_wholemount_weak.wlz

13078_wholemount_possible.wlz

13078_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13078_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9476
Entity Detected:	Dhx37, DEAH (Asp-Glu-Ala-His) box polypeptide 37 ( MGI:3028576)
Sequence:	sense strand is shown >T9476 GCGAGGGAGCCATGGGGAAGCTGCGACGCCGCTACAACGTGAAGGGGCGGCTGCAGGCCGATCCCCGGCC CGCCAAGGGCCCTGAAGCGCCTCCCGTACGGCTGGAACTGGAGGGCAAGGATGTGTTGAAAGGAGTTGAT GAAAGCAATGTTCTGGTGCTGCCTGGGAAGAGAAAGAAGAAGACCAAGGCGCCGCCCCCATCCAAGAAAG AGAGGAAGCCCCTGACAAAGAAGGAGAGGAAGGTGCTACAGAAGGTCTTAGAACAGAAGGAGAAGAAGAG CCAGCGTGCCGAACTGCTGCAGAAACTGAGTGAAGTCCAAGTATCGGAGGCCGAGATGAGTCTCTTCTAC ACCACAGCCAAGCTCGGCACTGGGGACCGCATGTATCATATCAAAGAGAGGAAGTCTGGCCAGCCTGCTG CCCGGGGCCAGGAGAAGGTCAGTAGCCTCAGCGGGGCCCACCGGAAGCGCCACAGGTCATCATCAACAGA GGAAGACTCTGAGTCTTCAGAAGATTCAGAGCCTGAGGAAGCCACAGCTGACCAACCTAGGACCAGCACA GGGACAGAGCCAGTGCATCCGCCACCAGCTCCTTCTGGGATCAACAGCCCTGCACCCACCCCGCAGCCCC CTCCATCTGGGATCTCTGCACCTCCAAAAACACCAGCCTCTGCCCCGCCTCCACCCGTGGCCAAGCCTGC TGTATTCATCCCCGTGAACCGCACCCCTGAAATGCAGGAGGAGCGGCTCAAGCTCCCGATCCTCGCCGAG GAGCAAGCCATCATGGAGGCGGTGGCTGAGCACCCCATTGTCATTGTGTGTGGCGAGACTGGCAGTGGGA AGACTACACAGGTGCCCCAGTTTCTCTACGAAGCAGGCTACAGCAGTGAGGACAGCATCATCGGTGTCAC AGAACCCCGCCGAGTGGCCGCAGTAGCCATGTCCCAGCGGGTGGCCAAGGAGATGAACCTGTCACATCGG GTTGTCTCCTACCAGATCCGCTACGAAGGGAATGTGACAGAAGAAACCAGGATCAAGTTCATGACGGACG GTGTGCTGCTCAAAGAGATCCAGAAGGACTTCCTGCTGCTGAAATACAAGGTGGTGATCATTGACGAAGC CCATGAGCGGAGCGTCTACACAGACATCCTTCTAGGGCTCCTGTCCCGCATTGTGGCTCTCCGGGCCAAG AGGCACCTGCCCCTCAAGCTGCTCATCATGTCGGCCACACTGCGGGTGGAGGACTTCACCCAGAACCAGC GGCTCTTCACCACCCCTCCCCCGGTCATCAAGGTTGAGTCGAGGCAGTTCCCTGTGACTGTACATTTCAA CAAGCGGACCCCATTGGATGACTACAGTGGTGAATGCTTCAGGAAGGTCTGCAAGATCCACCGGATGCTG CCTGCAGGTGGCATCCTGGTATTTCTCACAGGGCAGGCCGAGGTGCATGCCCTTTGCCGTCGGCTTAGGA AGGCCTTCCCCTTTCGTTGCTCCCAGCCACAAGAAAAGGAAGAGGACTCAGCAGAAGGGATGCGGAGGTT TAAGAAGTCCCGGACAAGGGCTAGGAAGGCCCAGGCCATGGCCTTGCCCCAGATCAACCTGGACAATTAC TCTGTGCTGCCAGCCGGCGAAGGTGATGAGGACAGGGAGGCAGAGATGGACGATGAAGAGGAGGCCCTGG GCTCTGACCTAGATCTGGACCTGGGTGACAGTGAGGCAAATGAAGGTGAGCAGCCGGATGCCTCCCTGCC CCTTCACGTGCTCCCGCTCTACTCTTTGCTGGCCCCTGAGAAGCAGGCACAGGTCTTCAAACCTCCTCCC GAGGGGACGAGGCTGTGCGTTGTGGCCACCAATGTGGCTGAAACCTCCCTCACCATCCCTGGTATCAAGT ATGTGGTGGACTGTGGGAAAGTCAAAAAGCGCTATTATGATCGTGTCACAGGCGTGTCTTCCTTCCGTGT CACCTGGGTCTCCCAAGCATCCGCTGACCAGCGGGCTGGTCGTGCAGGCCGCACAGAGCCAGGCCACTGC TATAGGCTGTATTCCTCAGCTGTTTTCGGGGACTTTGAGCAGTTTCCTCCTCCAGAGATCACCCGCAGAC CAGTGGAGGACTTGATCCTGCAAATGAAAGCACTCAGCATTGAGAAGGTCATCAACTTCCCGTTCCCCAC CCCGCCGTCCGTGGAGGCCCTCGTTGCTGCAGAGGAGCTGCTGGTTGCCCTGGGGGCCCTGCAGGCACCT CCAAAACAGGAAAGAATGAAGAAGCTACAGATGTCACAGCTGAGCTGCCCCATCACCGCGCTGGGGCGGA CCATGTCCACCTTCCCTGTGGCACCCCGCTATGCCAAGATGCTGGCCCTGAGCCAGCAGCATGGCTGCCT GCCCTACACCATTGCCATCGTGGCTGCCATGACTGTGCGTGAGCTGTTTGAGGAGCTGGACAGACCTGCT GCCAGTGAAAAGGAGCTTGCCGAGCTAAAGGGCCGCCGAGCCCGTGTGGCCCAGATGAAGAGGACCTGGG CCGGGCAGGGGCCATCTCTGAAGCTTGGCGACCTCATGGTGTTGCTGGGTGCCGTGGGAGCCTGTGAGTA CGCTGGCTGCTCACCCCAGTTCTGCCAAGCTAACGGACTGCGCTACAAGGCCATGCTTGAGATCCGGCGC CTGCGTGGCCAGCTGACCACTGCAGTCAACGCAGTGTGCCCCGAGGCTGGGCTTTTCCTGGACCCCAAAA TGCAGCCTCCTACTGAGAGCCAGGTGACTTACCTGCGGCAAATCATGGCAGCTGGTCTGGGTGACCACCT GGCCCGCCGGGTTCAGAGTGAGGACTTGCTGGACCCCAAGTGGAAGAATGCCTACAAGACTCCTCTTCTA GATGACCCCGTCTTCATCCACCCCAGCTCTGTACTCTTCAAAGAGTTGCCTGAGTTCGTGGTCTACCAGG AAATTGTGGAGACAACCAAGATGTACATGAAAGGTGTCTCCACCGTGGAAATCCAGTGGATCCCCTCCCT GCTGCCTTCCTACTGCCAGTTTGATGCACCCCTGGAGGAGCCAGCGCCCAGTTACTGCCCAGAGTCAGGA CAGGTGTTGTGCCACCGGGCCAGCGTGTTCTACCGTGTGGGCTGGCCTCTCCCTGCCGTCCAGGTGGACT TCCCTGAAGGCATTGACCGCTACAAATACTTTGCAAAGTTTCTGCTGGAGGGACAGGTCTTCCGCAAGCT GGCTTCATTCAAGAGCTGCCTGCTATCTAGCCCCAGCACCATGCTGAAGACCTGGGCCAGGCTGCAGCCC AGGACAGAGACCCTATTGAGAGCCCTCGTGGCCCACAAGGCTGATTCTCGTGATTCCCTGCTGGCTGCAT GGAAGAAGAACCCCAAATACCTGCTGGCTGAGTACTGCGAGTGGCTCCCAAAGGCTATGCACAGCGATGT TGAGAAGAACTGGCCCCCTACCACTGACAGTTGACCACACACCCAGCTGCACAGTCAACTGGCTCCTAGG CTGTTACCAGGAGCCACGCTGTGGCCCTGACAGCCCTTTTCTGCCCAATTCAGAAGCAAGCTCACAGCTA CACTGACCAGGAGTGCCCTTCTTTGTGACTGTTATAAGAGCAGACATGGTTTGTCTTCCACATTCCTACT GTCTAAGACGGACTCTTCAGGTGACATTAGACCCTGCAGATGCACAGAGCAACACTGAAACAGGAAAGTG GACCCTTCTCCCAGAGCCCTGTCTGGCCTCATAGCATCTTGGACAGCAGGTGTACTGCAGACTTCTGTGA CCCAGTGACTTTCCTGAAAGGTTAACCCCAGATGAGGGATGATCACCCCAGAGCTTTGCTCCACATAGAT GGAGACTGGTTTCCGGGGTCCTGCTTTGGCATCAGTAGACAGCAAGTTCTGTCTTTAGTGGGCCTTGGCT GGTAAGGATCACAAATGCAAGGCCAACCTGAGCTACACAGCAAGACTGTCTTCTATTTGTTTGGTTTTTT TTTTTTTTTTTTTTTTGGTTTTTGGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCACTC TGTAGACCAGGCTGGCCTCGAACTCAGAAATCTGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGTG TGTACCACCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30354591 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30354591 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13076 same embryo
	EMAGE:13077 same embryo
	EurExpress:euxassay_017285 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source