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EMAGE:13083

Plcb4 phospholipase C, beta 4 ( MGI:107464)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13083 EMAGE:13083 EMAGE:13083 EMAGE:13083 EMAGE:13083
"Pseudo-wholemount" of euxassay_017213. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017213_01 euxassay_017213_02 euxassay_017213_03 euxassay_017213_04
EMAGE:13083 EMAGE:13083 EMAGE:13083 EMAGE:13083 EMAGE:13083
euxassay_017213_05 euxassay_017213_06 euxassay_017213_07 euxassay_017213_08 euxassay_017213_09
EMAGE:13083 EMAGE:13083 EMAGE:13083 EMAGE:13083 EMAGE:13083
euxassay_017213_10 euxassay_017213_11 euxassay_017213_12 euxassay_017213_13 euxassay_017213_14
EMAGE:13083 EMAGE:13083 EMAGE:13083 EMAGE:13083 EMAGE:13083
euxassay_017213_15 euxassay_017213_16 euxassay_017213_17 euxassay_017213_18 euxassay_017213_19
EMAGE:13083 EMAGE:13083 EMAGE:13083 EMAGE:13083 EMAGE:13083
euxassay_017213_20 euxassay_017213_21 euxassay_017213_22 euxassay_017213_23 euxassay_017213_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13083Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13083_wholemount_strong.wlz
13083_wholemount_moderate.wlz
13083_wholemount_weak.wlz
13083_wholemount_possible.wlz
13083_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13083_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37948
Entity Detected:Plcb4, phospholipase C, beta 4 ( MGI:107464)
Sequence:sense strand is shown

>T37948
GCAGGTTATATCAGGGCAGTTCCTCTCAGATAAGAAGATCGGGACATACGTGGAAGTCGATATGTACGGG
CTGCCCACCGACACCATACGGAAAGAGTTCCGAACCCGCATGGTTATGAACAATGGACTCAACCCAGTGT
ATAATGAAGAATCGTTTGTGTTTCGAAAGGTGATCCTGCCTGACCTAGCTGTCCTGAGAATCGCAGTCTA
CGATGACAACAACAAGCTAATTGGCCAGAGGATCCTTCCTTTGGATGGTCTCCAAGCAGGCTACCGACAC
ATCTCCCTGAGAAACGAGGGAAACAAACCATTATCACTGCCAACAATTTTCTGCAATATTGTTCTTAAAA
CATACGTGCCTGATGGATTTGGAGATATTGTGGATGCTTTATCCGATCCAAAGAAATTTCTTTCAATCAC
AGAGAAGAGAGCAGACCAAATGAGAGCAATGGGCATTGAAACTAGTGACATAGCAGATGTGCCCAGTGAC
ACTTCCAAAAATGACAAGAAAGGCAAGGCCAACCCAGCCAAAGCGAACGTGACCCCTCAGAGCAGCTCTG
AGCTCAGACCAACCACCACAGCCGCCCTGGGCTCTGGCCAGGAAGCCAAGAAAGGTATTGAACTTATCCC
TCAAGTGAGGATAGAAGATTTAAAGCAAATGAAGGCTTACTTGAAGCATTTAAAGAAACAACAGAAGGAG
TTAAACTCTTTAAAGAAGAAACATGCAAAGGAGCACAGTACCATGCAGAAGTTACACTGCACACAAGTTG
ACAAAATCGTGGCCCAGTATGACAAAGAGAAGTCGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 101949. Forward Primer - name:101949_F_cDNA_Plcb4, sequence:GCAGGTTATATCAGGGCAGTTC; Reverse Primer - name:101949_N_SP6_cDNA_Plcb4, sequence:TCGACTTCTCTTTGTCATACTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13082 same embryo
 EMAGE:13080 same embryo
 EMAGE:13079 same embryo
 EMAGE:13081 same embryo
 EurExpress:euxassay_017213 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS