EMAGE mouse gene expression spatial database

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EMAGE:13094

Ier2 immediate early response 2 ( MGI:104815)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013742. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013742_01	euxassay_013742_02	euxassay_013742_03	euxassay_013742_04

euxassay_013742_05	euxassay_013742_06	euxassay_013742_07	euxassay_013742_08	euxassay_013742_09

euxassay_013742_10	euxassay_013742_11	euxassay_013742_12	euxassay_013742_13	euxassay_013742_14

euxassay_013742_15	euxassay_013742_16	euxassay_013742_17	euxassay_013742_18	euxassay_013742_19

euxassay_013742_20	euxassay_013742_21	euxassay_013742_22	euxassay_013742_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13094_wholemount_strong.wlz

13094_wholemount_moderate.wlz

13094_wholemount_weak.wlz

13094_wholemount_possible.wlz

13094_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13094_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 16 17 18 19

vibrissa

strong

regional

strong expression: see section 04 05 06 18 19 moderate expression: see section 07 08 20 21 22 23

inner ear vestibular component

strong

regional

strong expression: see section 06

cochlea

strong

regional

strong expression: see section 07 08 09 17 18 19

utricle

strong

regional

strong expression: see section 07 18 19 20

lower jaw incisor

strong

regional

strong expression: see section 11 12 15 16

lower jaw molar

strong

regional

strong expression: see section 06 07 19

upper jaw incisor

strong

regional

strong expression: see section 10 11 12 15 16

upper jaw molar

strong

regional

strong expression: see section 06 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31658
Entity Detected:	Ier2, immediate early response 2 ( MGI:104815)
Sequence:	sense strand is shown >T31658 AAGCCGAAGTTGCAGTGGAAGTAGCGTCCCCCGAAGCCGTGCAGCCTCCGGAGCCCATGGATACGCAAGA GGAAGTGCTGCGAGTCCAGGAGACCCCTGCGCTCTGTGACCCGCCCCCCGCTAGAGTCAGCCGCAAGCGC CGGAGCAGCAGCGATTTGAGCGACGGTAGTGATGCCGGACTGGTACCAAGCAAGAAGGCCCGTCTAGAAG AGGTGGAGGGGGAGGCGACGTCGGAGGTTCCCGATCGCCTGCAGCTTCCTCCGGCACAAAGCGAAGGTGC CTTCCCTAACCTCGCCCGCGTCCTCCAAAGGCGCTTCTCCAGTCTCCTGAACTGTGGACCCGCCGTGCCC CCGCCGACGCCCCCCACGTGCGAGGCCAAGCCAGCCTGCCGCCCGGCCGACAATATGCTCAACGTGCTGG TGCGAGCTGTGGTGGCCTTCTGAGAGCTCTGGTGGCTTCTTTCGAGCGGCGCCACCGGAGCGGAGAACGC ACACCCGAGGCGAAGGCCGGCGGGGGCCGTGAAGAAGAGCCGCGGCCCGAGCTGCCGAGAGGCCAGGGGA AGGACTGAGGAGCGAGGGGCGCGGGCGCCTTCTCCCAGACGTGCGTCCATAGGTGCTATTAAAGGACTGT CCCTTCCTTGGCTTGGAGAAGGGACACCTAGATCTTGAATCTCAGGGTCGAACTCTCTAGGGGCCAGGCT GCCCTTTCAAGGCCGTTTCACTACCATTCGCGTTTCGGCCCCTACAAGTGGGCACGCTTGTGCAAGCGGT CAGAGTTGCGTCATGGGACAGACGCGGGTGCTTCCTGTTGCCTTGCG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3590296), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 11426. Forward Primer - name:011426_F_IRAV08-11_M03_Ier2, sequence:AAGCCGAAGTTGCAGTGG; Reverse Primer - name:011426_R_SP6_IRAV08-11_M03_Ier2, sequence:ACGCAAGGCAACAGGAAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13093 same embryo
	EurExpress:euxassay_013742 same experiment
	MGI:4825503 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source