EMAGE mouse gene expression spatial database

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EMAGE:13104

Rrm2b ribonucleotide reductase M2 B (TP53 inducible) ( MGI:2155865)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017304. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017304_01	euxassay_017304_02	euxassay_017304_03	euxassay_017304_04

euxassay_017304_05	euxassay_017304_06	euxassay_017304_07	euxassay_017304_08	euxassay_017304_09

euxassay_017304_10	euxassay_017304_11	euxassay_017304_12	euxassay_017304_13	euxassay_017304_14

euxassay_017304_15	euxassay_017304_16	euxassay_017304_17	euxassay_017304_18	euxassay_017304_19

euxassay_017304_20	euxassay_017304_21	euxassay_017304_22	euxassay_017304_23	euxassay_017304_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13104_wholemount_strong.wlz

13104_wholemount_moderate.wlz

13104_wholemount_weak.wlz

13104_wholemount_possible.wlz

13104_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13104_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9172
Entity Detected:	Rrm2b, ribonucleotide reductase M2 B (TP53 inducible) ( MGI:2155865)
Sequence:	sense strand is shown >T9172 CCACCGCAGAGAAGGCCCCTCAATGGGCCGGCTAGAACCCAGGACCGGCGCGGCAAGGCGTGCCCGGAGC AGGAGGAGGTTCGCACAGGACGATCTGATCCTACTAGGATTTGGTCGGCCGCGCTCAGTTCCTCAGCTCG GGTGGCTCTGGTGGAGTCTGCGCGATGGGCGACCCGGAAAGGCCGGAAGCGGCCAGGCCGGAGAAGGGTG AGCAATTGTGTTCGGAGACAGAAGAAAATGTGGTGAGGTCAAATGAAGAGCCACTCCTAAGAAAGAGTTC CCGACGGTTCGTCATCTTTCCAATCCAGTATCCTGATATCTGGAGAATGTACAAGCAAGCACAGGCATCT TTCTGGACAGCAGAGGAGGTTGACTTGTCAAAGGACCTCCCTCACTGGAACAAGCTTAAATCCGACGAGA AGTATTTTATCTCCCACATCTTAGCCTTTTTTGCAGCCAGTGATGGAATTGTAAATGAAAACTTGGTGGA GCGTTTTAGTCAGGAGGTGCAGGTTCCAGAGGCTCGCTGCTTCTATGGCTTTCAGATTCTCATCGAGAAT GTTCACTCAGAGATGTACAGTTTACTAATAGACACTTACATCAGAGATCCAAAGAAAAGGGAATTTTTAT TTAATGCAATTGAAACAATGCCATATGTTAAGAAAAAAGCGGACTGGGCTCTGCGCTGGATAGCAGATAG GAAATCTACTTTTGGGGAAAGAGTGGTGGCCTTTGCTGCTGTGGAAGGAATTTTCTTCTCTGGATCATTT GCTGCAATATTCTGGCTAAAGAAGAGAGGCCTCATGCCTGGACTGACTTTTTCAAATGAACTGATCAGCA GGGATGAAGGACTTCACTGTGACTTTGCCTGCCTGATGTTTCAGTACTTGGTAAACAAGCCTTCAGAAGA TAGAGTGAGGGAAATCATTGCTGATGCTGTTCAAATCGAGCAGGAGTTTTTAACAGAAGCCTTGCCTGTT GGCCTCATTGGAATGAACTGTGTTTTGATGAAACAGTACATTGAGTTTGTAGCTGACAGATTACTTGGAG AACTTGGATTCTCAAAGATTTTTCAGGCAGAAAATCCCTTTGATTTTATGGAGAACATTTCATTAGAAGG GAAAACAAATTTCTTTGAGAAACGAGTTTCTGAGTATCAGCGATTTGCAGTCATGGCCGAAACCACAGAT AATGTCTTCACCTTGGATGCAGATTTCTAAAGCCCTCCTCATTTAAAAAAAAAAATCTATAGAATTCGGC TGTGGTAGTACATGCCTTAGTCCAGTCCTCAGAGGAAGCTGAGGGCACGTGGATCTCTGGGTTTGAGGAT GATCAGGGATACATACTGAGATCTATCTCAAAAGCAACAGAACAGCGAAAACAAACAAAACACATGGAAA CCTGTTGTTGGTAAATAGCAATCTATTTTTGTCTTTTTGAAACTTTCAGTATCTCATTTAAGGGAATGAG AATTTGAATAGAAGGAAATATAAAACTAAATTCTAAATGATTTAATTTTATCTAAATGTACTTCAGTTTA TTTATACAATGAAAGTGTGACCAATATGTCTTTTTTTTTTCCTTTTTGTCATAAGCACTTAAGATGGCAT TAAGAGCATAGATTTAGGAGTCAGGTATGAGCTTGATATTCTGGCTTACTCCTTGATGTGACCTTGGCAA GTCAGTTAGTCACTGTAAACGTCAGGATATTTATCTGTAAAATTTGTATAATAGTAAGTCCCAAGTTCAT GGAGGTGACCAGAGGGCTAATATACAGAAATAGTTTGTTTATCTAATACTTTTATCTCCACTGCCTCACC TGCTAATTAATATTAGTGAGAAGATCAAGTACAGTGGCGTTGTGATGCAGTCCACTCCTCGCCAGAGAGT GACTGAGGGGTGGATTGAGATAAAGAATTCATTGAATCTTTGTGGTTTTAATGTTTGCAGGAGGAATATA AGGGTCTCCTTAGAGAGATCATAATTCTTGTCAACTGCTTTGAATTCATTAATCTGCAGTAAAGAAATAA TATATAGCTCTTAATTGCTTATCGTGGGAGGCTTATGGAGTAGTAGACTCCAGGTTACCATGGTTGTGGT TCTTGTAAGCAAAGTGATTCTTCTTTAGGAATTAAAAGGATAACTGTTGCCAGGGACAGCCCAGGGGTGT TTAGAACCTGGATTGATGGGCATTTCTCAAAGTTGTCTTCTCAAACCTGGTAGATCTTCAGAGTTCTTGT GATGGAAAAAGAGATGCAGTCAGTGGGCTTGGGGAAGGCTGAGCATTCCATCCCTGCCTAGAATGCAGTC TGCAAAGCAGCTGCCCACATGTACACAACACAAGGCTGTCAGCACCTCCCACAGCTACTGAGTGCACTGG GGAAAGCTGTCCTAGAATCATTTATACACACTGGAAATGGAGAAAAGAAGGGAGGTGGGAGGGACTAGTG AACCATTTTGGATTTGTGCCAATCTGAGAAAGTGATTTAAGGTTGACGTCATCTTTATATCCTTATACTC TTAAGTCTCTACACTGTTATAAAGGAATGTTCTTTTAATGGACTGTCTATAAGGGAGATGGAAGTCTTTC TAAGAAAGATGCAGAGAGGCCAGGAGCCAGGACACCTGGGCAATGTGCCCTTCCTCATTTTCAGGAAAGT ATCAAGAGGAAGCCTAAACCCCATCAAACCCGGTCCCTGGCTCAGTAAAGTTCCTGCTACTCAAGTGTGA GGTCCTGAATCCAGATCCCTAGCATAGCACACACGCACACACGTACACATGCACACACGCACACACCACC TGTACATGCACACATATGCACACACACAGAGTACAAATAAAATGATGGCAGCTGAAATTCAGAGGTTTAT CTCTGTTGAAGTTTTTATTCTATTTGCTCTACAACTATCTGATCAAAACTCGGAATCCTAAATGAATGCA AGCCATACTTCCATTGTTAAAGGGAGCACCAGAAATGAAACATGGCTCTGTGCTTCCCATTTGTCATGTT ATCACCCAGTAGCCCTCTAAGCACGACAGAGTAGCTATTACTAAAACATGGAGAGAGGAAGGAAGGCATC AGAGAAGCTGAAAAACTTAACTTTACAGGCACTGTCCTATAATATGTTGAGACTGTTACATCCTTTAAAC TTCATCCATCATGATTTATTTTGTCTATTCTTTCATTAATTAAACTATTGCATACTACTATAACAGAGTA TCAAAATATCATAGAACTCGGGGTATGTGGGTAGTATATTCCAAGGTATTCATCCACTATTATACAAAAA CAGCTAAAACTTAAATTACCACAGACTGAAAGAAAACAGTTTTAAAGGTGTACATTCTCAAAGTTTTAAA CCCGGTTCTTCATAAGTAGTGACAGGAACCTTTATGTGATACATGTTAATAGTAGCAATAAGCAAAACTG TATTTGAAAGAAGTCTTAATTCTGAAAAACTGAATTCTTAGTACACTGTTACATTACTACTCTCAGTATT CTGTATCCCTTCAGTGCCTCCAGGTTGCACATTTATTTATTATGTTTAGTATTTTGGTTCTTAGATATGG AAAAATCAAGTTATAAAATATCTTTTTAATATCTCACAGATAACAAAAAAATCAAGGTAATGCCATCTCT CTCTCTCTCTCTCTCTCTCTCTTTTTTTTTACAGTACATGCGTTTCAAAGAAGTGCAATTTGTCCTAGAG TTATGTTTTTATATTTCAAGATGATAAACAGCTTTGTTATGACCATAGTTTAAATAAATGTTTCAACACC TAGAATCATGATGTAGAGGAACTTTGGATGAATTTTTTGCAGGGCTGAGTTTCAATAATTAAGAGTTATT TTTGTGGATAGATCACTCCCAGCCATATCTTATTATTTGAACCTGTTACAGTTGTTTCATTTAAAAATCT CCAGATTCTATTTATGTAGCTACTTTATTATGTTTATTTTCCTTCTTTTAGTTGGTGTCTCATGTGGTCT GGCCTGGATCTCACACATAACCCTCTTGCTTCAGCCTCCCAGTCCCTGGGGTTATTGGTGTGTGATGCCA TGCCCAGCTACCACTTTATGTTCTGTGCATGAAGAAATGTAGTGTTCATATTTAAAAATAATTTAATATT CAAAATTTTGTATTTTATATTTAAATGTTATTATGTCATTTTCAAAGACTCCAGATTTTTTTCACAATTA AGCAAAGACTCCAATGTCACTTCATTGATATGGAAACTCTTGATGGGCTACATTCAGGACAAATTAGTTC TAAAGTCAAAAATCATGTTTTGAATTCACTGAGGGAGTTGTCACCCAGCTCAGACAGAACTTGGGGCTGG TAATGATCATTTAACTAAATTACTAATTATTTAAACAAGTTTATTAGACATGGTGTTCCTAAATATATTT TGTTAAAATACATTATGGTAGTAAAACCAATTCTGCATGTAGAGTTTGAGGTTTTTTTGCTCTGAAAAAT TATGACTTTTAAAATAAAGTTTACTGAAATAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827753 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827753 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_017304 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source