EMAGE mouse gene expression spatial database

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EMAGE:13176

Ranbp3l RAN binding protein 3-like ( MGI:2444654)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017386. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017386_01	euxassay_017386_02	euxassay_017386_03	euxassay_017386_04

euxassay_017386_05	euxassay_017386_06	euxassay_017386_07	euxassay_017386_08	euxassay_017386_09

euxassay_017386_10	euxassay_017386_11	euxassay_017386_12	euxassay_017386_13	euxassay_017386_14

euxassay_017386_15	euxassay_017386_16	euxassay_017386_17	euxassay_017386_18	euxassay_017386_19

euxassay_017386_20	euxassay_017386_21	euxassay_017386_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13176_wholemount_strong.wlz

13176_wholemount_moderate.wlz

13176_wholemount_weak.wlz

13176_wholemount_possible.wlz

13176_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13176_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9282
Entity Detected:	Ranbp3l, RAN binding protein 3-like ( MGI:2444654)
Sequence:	sense strand is shown >T9282 TTTTGAACATTCTAAAAGGGGAAAGGTCCAGGGCGCTTTAAAATAAACTAAGAGTTAAGTCGTGTTTCCA GCCAAATAAGCTGGCATCTTTGTAGGCAGGAGGTGCGATACTGGTAAATATTTACTGCAGAATTCTTGAC CAGTAGAAACCCTGGCATGCTTTTCGAGGAGTAAATGTTTTACATGCGAGGCGAGATCTGCGCGTGCCTC TGTGTTCTTTCCAAGTAACTTCTGAAGACAGTTTTTGATGTCTGGCTAAGACAAACTGCGCTAGTGGTCC CCGAGTCACAGCCAGATCGTTGGTACCATGTCTACAACACAAAGGAAAGACGACAGCCACCTGTTCACCA GTTCGTGCACGCGTCAGCTGCAGGTGCAGGAGGATCGGCAACAGCAAGAAAAATATGTCATTGCTCAGCC AATATTTGTTTTTGAGAAAGGAGAACACAATTTTAAGAGACCTGCAGAAGACAGCCTGGAAGAAACGGCA GAGCCTGAATTCACTGGTTTCTTGAGAAAGCGTGTAAGGTCTTCATCTGTTACGCTTCATACTACAGATC CTCAGTCTCAAGGAGTGGCAACTTTGTCTCAAACGCGTCTGAGGTCCTCGTCATTCACTGATGTCCCGAC CTTCCCTCCATGCAGGCCAGTGAGGAAAAACAATGTTTTTATGACATCACGTCTGCTGCAAAGGAGTGAT GACATGAACAACGTAGAGCAAGGACCCCCCATGAGATCTTCTGAACAGGTTCTAAGACCCGCTGTTCTGC AGCCGTCTCAAACTCAGAGTTGTCAGAAAGCAGGCACAACATTTGGGCCCGGTGCATTGAAATCCTACAA AACTAAGGAAAAGGCCGAGCACGAGATTTCTGAAGTTGGAAGTTCCTCTAGTTTACTAAGTGAAAATTTA CCAAATGCCAGAAGCTCAATCCAGCTGTCTACTGATCCATGCATCTCAGAGGCACCATCGGGATGCCAAC CAAAGGAAGATAAGTGTTCTTTTACAAGCTGCAGTTCTGACTTCGTTTTTGGAGAAAACATGGTAGAAAG AGTTTTGGGCACTCAAAAACTTACTCAGCCTCCACTTCAAAATCTTTCATATGCCAAGGAAAAAACATTC AAATCTGTTCTGAAATTCCCTAATGCTGTTTCAAATTCAGACTCTATTGAAAACATATCCCTAGTCGAAT CAGCTGCTGCTTTTTCCTCCAAACCATCACAAAAATGCTTGCTTGAGAAGATTGATGTTATAACAGGAGA AGAAACTGAACATAATGTGTTAAAGATCAACTGCAAGATCTTTGTATTCAACAAAGCCACAGAATCCTGG AGTGAAAGAGGCCAAGGGATTTTGCGACTGAACGACACGGCCGGCCGAGAGTGTGGAACACTGCAGTCAA GACTAATCATGCGCAACCAAGGCAGTCTGAGGCTGGTCCTCAACAGCAGACTCTGGGCTCAGATGAAGAT TCAAAGAGCAAGTCAGAAAAATTTGCGAATAACTGCGACTGATTTAGAAGACGATGGTATAAAAATATTT TTAATTCAGGCTAGTGCCAAAGATACAGGCTTTCTGTATGCAGCAATCCACCACCGTCTGGTCGCGCTTC GAAGCCTCGCTAAGCAGGGTGACGGTGGTCCAGCCGAAAGCCAGTCCGATACAGCCCTCCCTCAGCTCAA TGGCGAGAGCTGTGACGAGGATGAGGATGAGATCGCACAAGTCACTAAAAATGGATCAGATCCTTCAAGG TGGAGCCACAGACAGTCAATTGTCTGTTCATAAACCCTCTCTGCTATAAACATGACATCTACAGAAAGAG TGGCCACTCTATTGAAAGGCAAGACCATCCTAACTGAGAAGACTCTACTCATTCATACCTTAAAGAGTTT TTTTAATTAAAGAAACAAACACAATTTATTGTAATTAAAAGTTTTAAGTTTTCATTATTGTGGACAGAAT TTAAGAAATGGCCTTCAGAATTTATCCTTTTGTGGATTACTCTAGAATAAGATTTGATAACTAATTTGGT CTATTCATTACACTGACAGATATATTAATTAAAAGTACAAATTGTCATCTTTGATAAATCTTTGATACAT ACCTAAAGTATTTATTTTAAGTCACATATTGTCTGGCTATAAAATATAGAATATAGAATATATAGAATAT AGAATATTTTACTAAATTTACAGTAATCTCTTTGGAAAATACCATAACTGCAAGGCATTTTATTAAACCA TGTGTATATTCTGAGAAATTTGTATTTTCACTTACACTTTGAATTTCCTAATACACACAAGAATTCTTTT ACAAGAGTGCCCATTCATGGACTTTTATCCATTGCATCTTATGTATTTGACTCAAAGTCAGCTCACAAGA GTTGATGAATAATTGAACAAAATGTATTACCTATGTGTCAAAAAATGTACAGAAACCAAATATCAATGTG CTTTATTTAGGTGGTCGTCTTAATAGTCTAGTAGGTGTAAGGCTATGGTAGAAGCCATTTTTTAACAAAT GTCTCCTATATATATGGGCAAATGAGTATCTCAAATTCCATCATTATGATATAGTAATTTTAATAATTGT TAGCAGATGCTTTATACCTTTTTGATATTTCAGAGACATTTTATGAATCATGCATGAAAATCCATGTCAG AATTGAGGGCAATATTTTATTTTATTAGTAGCCCCAAACAATCTCATTACAGGATAAAGATTCAAGGTCA TAAATGATTTTGGATGTGTTCTGAATCTTGATATGCTTGTAATAAGCCTGAGACTTTACAAACTGTTACA CTGAGGAAAATATTCTTTGAAAGCATACATATGAATATATATTTTAAAAGTTGACAATGCTTTATTGCCT CCATACAACAGAAGTATCCGCATGTGCCTATCTATTAATCAAATCTCAGCTTAAATCTGAAAGTCTGGCA TACAATCGCTGAGTAAGGTCTTTTAGAGTAGGATTTAATTAATATGTTCAGAAGAAGATAGAAAAGGTAT CAGAAGAAGAGAGAAAGACTTGCCATCAGGAAAAGGAGTAAACGCAGATGATTAAGATGATCTCCCCTCC CTCCCACTCTGTGGCAGAATACAAATCATAGGATGAGTCTAACAAGAAGCATCATCAGAGAGGCATTTGA AGAAGATATGAAAGTACTGCTTCACCCATAGGATGTTAGCTTTGAGGGAACATTCTGATTGTATGGTGAT TTTTAAAGTGAAGCAGAGAACAGAGGTCTTCTCTATCCCTTGGCATGTTTCTTCTTGGTCCCATACTGGA AATGAAATGAAGTAAAAAAAAAAAAATTGTGTGTTCCAAATACTTTAATAGAAGTTGAGTCGACTGATTT TCTTTGGTCTTCTGTACAGATGGCAAATGAGAAAGAGACTTACTTGTAACCCATACAGAAATGCCAGGAG GTGCCCAAGTAGGTTAGAATGTCAGCATGGCAGAGAGACAGTGTCAACCTCAGGGTCATGAAACGGTGAC AAGGAGCAGAGACATGACATACATTGCAAGAGTCCTCCATCTTTTTTTTTTTTGTTGTTGTTGTTTGTTT GTTTGTTTTGTTTTTTTTTTCGAGACAGGGTTTCTCTGTGTAGCCTTGGCTGTCCTGGAACTCACTTTGT AGACCAGGCTGGCCTCAAACTCAGAAATCTGCCTGCCTCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAG GTGTGTGCCACCACGCCCGGCTGAGTCCTCCATCTTGATTAGGAGAAACATTTCCTAGATTTTCTTTATC TTGTATTTTCATCTAGCCTCCTCCTGCCCCCACTTTTTGGGTCTTCTACACTCTTTGTCTCCTTTCAATG TGAAGCAATCAACTATACAATAATATTCATTTTCATAAGTTATTCAGACAGGTGAGTGCAAATCTTACAT TCTGGATTTCCATATAAGCTATTTTTTATTAAAATCTGATAAACTGATTTCCAAATTTATTACATATGTA TCTAAATTTATATTGAATTATATATCTTATATAAAGATAATCTAAATATTGTCTTTCAATAGTATTTACT CTAATTGTTTAGCATAATTGATGAATCATTAAAACATTTCCTGCTACAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6839130 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6839130 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13174 same embryo
	EMAGE:13175 same embryo
	EurExpress:euxassay_017386 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source