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EMAGE:1319

Foxj3 forkhead box J3 ( MGI:2443432)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:1319 EMAGE:1319
0040b.jpg This embryo was treated with ProteinaseK for 30min. 0040a.jpg This embryo was treated with ProteinaseK for 3min.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:1319Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1319_wholemount_strong_3D_1.wlz
1319_wholemount_moderate_3D_1.wlz
1319_wholemount_weak_3D_1.wlz
1319_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1319_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
central nervous system
possible possible
Authors stated that in some instances they could not determine expression levels with certainty.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3505933
Entity Detected:Foxj3, forkhead box J3 ( MGI:2443432)
Notes:The template DNA sequence was PCR amplified from E13.5 or P0 mouse brain, embryonic kidney or testis cDNA using: 5' primer: cgcggatccccagctcaccatgagagcagc 3'primer: ccgctcgagtggctgctgctgctgctgagg Editors Note: Original information from the authors states that the two primers were used to generate a 705bp fragment (nt342-1049 in NM_172699). Pairwise comparisons of these two primer sequences against both versions of NM_172699 identifies a 729bp fragment in each case (nt294-1023 in NM_172699.1 and nt266-995 in NM_172699.2).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Yu J., Tenzen T., Gray P.A., Fu H., Luo P., Zhao Q., Ferrari A., Yuk D.I., Tsung E.F., Cai Z., Alberta J.A., Cheng L.P., Liu Y., Stenman J.M., Valerius M.T., Billings N., Kim H.A., Greenberg M.E., Rowitch D.H., Stiles C.D., Ma Q., McMahon A.P. Indexed by GXD, Spatially mapped by EMAGE.
Principal investigator:Andrew P. McMahon, Molecular and Cellular Biology, Harvard, Department of Molecular and Cellular Biology Harvard University 16 Divinity Ave Room 1059, Cambridge, U.S.A MA 02138
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1126/science.1104935] [ PMID:15618518] Gray PA, 2004 Mouse brain organization revealed through direct genome-scale TF expression analysis. Science (306):2255-7
Acknowledgments:This work was supported by the Charles Dana Foundation, the National Institute of Neurological Disorders and Stroke (QM, CDS, DHR & APM), the National Institute of Dental and Craniofacial Research (QM), the National Institute of Diabetes and Digestive and Kidney Diseases (APM), a Ford Foundation Postdoctoral Fellowship for Minorities (PAG), a Parker B. Francis Fellowship in Pulmonary Medicine (PAG) and the Pew Trust (QM).
Links:MGI:3508656 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE