EMAGE mouse gene expression spatial database

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EMAGE:13199

Gnl3l guanine nucleotide binding protein-like 3 (nucleolar)-like ( MGI:2448557)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_017367_01	euxassay_017367_02	euxassay_017367_03	euxassay_017367_04	euxassay_017367_05

euxassay_017367_06	euxassay_017367_07	euxassay_017367_08	euxassay_017367_09	euxassay_017367_10

euxassay_017367_11	euxassay_017367_12	euxassay_017367_13	euxassay_017367_14	euxassay_017367_15

euxassay_017367_16	euxassay_017367_17	euxassay_017367_18	euxassay_017367_19	euxassay_017367_20

euxassay_017367_21	euxassay_017367_22	euxassay_017367_23	euxassay_017367_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13199_wholemount_strong.wlz

13199_wholemount_moderate.wlz

13199_wholemount_weak.wlz

13199_wholemount_possible.wlz

13199_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13199_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9231
Entity Detected:	Gnl3l, guanine nucleotide binding protein-like 3 (nucleolar)-like ( MGI:2448557)
Sequence:	sense strand is shown >T9231 CGACGTTGTGCTTCTAACAGCTCTCCGAGGTCCCTGCCGGAAGTGTAGGAAGAAGCAGACAGATTTGAAC ATCTCTGTTTCCAGCTTCTCTGATCATCATGATGAAGATTAGACACAAAAACAAAAAACCAGGTAAAGGT TCCAAAGGCTGTAAGAAGCCTGCAAGGCAAAATGGGAAGAAAGTAACCTCCAGACCATCATCTGCTCCCC AGATTGTTCATGGCAATGACCATGCCAGTCGCGAGGCCGAATTAAAGAAGAAAAGGGTCGAGGAGATGAG GGAGAAGCAGCAAGTTGCCCGGGAGCAAGAGAGACAGAGACACAGGACCATGGAGAGCTATTGTCAGGAT GTCCTGAAACGTCAGCAGGAATTTGAACAAAAGGAGGAAGTTTTGCAGGAATTAAACATGTTTCCTCAGT TGGATGATGAGGCCACAAGGAAGGCCTATTACAAGGAATTCCGGAAGGTGGTAGAGTACTCTGATGTGAT TCTGGAAGTCCTAGATGCCAGAGACCCATTGGGCTGCCGCTGTTTCCAGATGGAGGAGACTGTCCTTCGT GCAGAAGGCAACAAGAAGCTGGTCTTGGTCTTAAATAAGATAGATCTCGTTCCCAAGGAGATTGTGGAAA AGTGGCTGGAATACCTTCTCAATGAACTGCCAACTGTGGCTTTCAAGGCCAGCACCCAGCATCATCAGGT CAAAAACTTGACTCGTTGTAAAGTTCCAGTGGACCAGGCCTCTGAGTCGCTTTTGAAAAGCAGAGCCTGC TTTGGAGCCGAAAATCTCATGAGGGTCCTGGGGAACTATTGTCGCCTGGGGGAAGTGCGTGGCCACATTC GTGTGGGTGTTGTAGGCCTTCCCAATGTGGGGAAGAGCAGTCTGATCAATAGCCTGAAGCGCAGCCGTGC TTGTAGTGTGGGAGCTGTTCCTGGTGTCACAAAATTCATGCAGGAGGTCTACCTAGACAAGTTTATCAGG CTTCTGGATGCACCAGGCATTGTCCCAGGACCCAATTCAGAGGTGGGCACCATCCTGCGTAATTGCATCC ATGTGCAGAAGCTGGCAGACCCTGTGACCCCGGTGGAGACCATCCTTCAGCGCTGCAACCTGGAGGAGAT TTCCAGCTACTATGGTGTATCTGGATTCCAGACGACTGAGCACTTTCTGACTGCAGTGGCCCATCGCTTG GGAAAGAAGAAGAAGGGAGGTGTATATAGTCAGGAACAGGCTGCCAAAGCTGTGCTGGCTGACTGGGTGA GTGGGAAGATCAGCTTCTATACACTACCACCGCCCACTCACACTCTGCCCACCCATCTCAGTGCTGAGAT TGTTAAGGAGATGACTGAGGTCTTTGATATAGAAGATACTGAGCACGCCAATGAAGACACCATGGAATGC TTAGCTGTGGGAGAATCCGATGAGCTGTTGGGTGACATGGACCCACAAGAAATGGAGGTCAGGTGGCTCC ATTCTCCACTGGTGAAAATAGCAGATGCTATTGAAAATAGAAGCACCGTGTATAAGATTGGAAATCTCAC TGGGTATTGTACCAAACCAAACCGTAATCAGATGGGGTGGCCTAAACGCAATGTGGACCACCATTGCCCC CAAAATAACCGTGTAGTAGAGGTCAGTTCTGTGGACCGCCGCCCGATGTTGCAGAGGATCCTGGAGACAG ACCCACTTCAGCAAGGCCAGGCTCTGGCATCTGCCTTGAAGAATAAGAAAAAATTGCAGAAGCGTTCAGA TAAAATCGCCACTAAGTTGTCTGACTCCATGATGTCCATGCTTGACCTCTCTGGCAACTCCGATGACTGT GCAGGTGACTGAGCAGCTGACCTTCCCCCTCATACTGCAAGTACTGCTTCCCGTGCGATGGGAGAGTCCA GATGCCTTTCCATTCTCCCTGATGTACTTAATAGGAGAACACCTCCATGTCATGTATCTTCTGCTGCCGT GCCCTCCATTCTTATCAAAGGTCTGGAGCTGCCAAACCCTACTACCCTCTACTTAATCCTTCCTGTCTTT GAAAATGACCTTGTGTTTGTTTTGTGGTGCCAGGGATTGAACATAATAGGTTCTCACAAATGTTAGACAA CCATTCTATTACCAAGCTGTAGTATTACCATCATTACCATCCATCATTATCATTGGGGAGTCTGCTTACT CAACCTCCCAAGTACTGGGATTACAGGCTTTTGCCACCATTCCCAGCTCCCACTGTGTTTTCTAAGGTAA ATAAAATCAGCAGACTCTTGGCATGGTGGCAACATATCTATAACCCTGACACTTGGGAGACTGGAGCTAG GAGGACTGTAAGTTATGGGCCAGCCTGAACTATCTGGCAAGACCCTTTCTCAACAAAACAGCCCAAAACA AATAAGCAGAGTCCAGCTGGGCATACACCTTTAATCCTAGCACTCTGGAGGCAGAGGCAGATGAATCTCG TGAGTTTCAGGCCAGCCTGGTCTACCGAGCAAGTTCCAGGATAGCCAGGGCTGCATAGAGAAACTCCGTC TCGAAAAGTGAACAATAAAACAACAAAAAGAATTTGTAATTATTTAAAAAGCAAATAAGCAGAGTCCAAG TGCTGACAATGTACCTTGGACTCTAAATTCCTGTGTCGTACCCTGTGAGTTAGGCTGATAAGGGTTAACC TTTTGGACAGGTAAATGTCCTAAAGGCTAGACTGCTGAGAAACCTGGTAGCAGTGGCGGGGGAGGTGGCG GTTGTCCCTGGATGGGTGATCCTTTTCATTTTTATCACCCTACAAGATCTCAGTGCCAAGGCTCTTGCCT GTGTATTTTCCAAATCAATAGCTAGCTCCTCATCTGGGTAGTGTAGGAGTAGAGAAGGATAGTGTGAGTC TCCTGTCTCTGGAAGTGTCTGGGGATAGTGTAGAGGATGACGATTCTAGAAAGGGTATACCTATCATAGT AAGTTGGAAAGCCATTGGCTGCAGGATGTTCAGGAAATGTAGACCGTACCTGTTTTCTGGACGGTTAGGG GCTGAGCACAAACTACTAAACAATGAGAAACAGTTACCACCTTTAACTTTCACTATATCCGTATCAGGAA GGGGTAAGGCTCAGAAAGCCGACAGAATGTGCTCATGTCAACAGTAAGTGGTGGTGTGCTAGATGTCAAA CTTGAGTCTAATTTAAACCTTATGCTAGCCTTTTGGTTTGCATTATTTTGCCTTTTCAACAAGGGCACAC CACTCTGGTATTTTCTGTTGTCATATATTTAAAATATATTTTCTCTACTCTGTTTTTTATTTTTATTTAT TTATTTATTTTTTGGGTTTTTCGAGACAGGGTTTCTCTGTATAGTCTTGGCTGTCCTGGAACTCACTTTG TAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTACCTCTGCCTCCCAAGTGCTGGGATTAAAGGCGTG CGCCACCACCGCCCGGCTCTGTTTTTTAAAAAATGGTAGTATTTATTGAAAAACTATGCTTTCTAAGTCT CTTTAAATGATGTCCAACTTAATTCACTGATCTCATCTTAAGTTTTGCTAGACAATAATATATTAGAAAT AAAAGTATCTAGATTCTTGGGAACATAATCATGTCAAAATAAAATAGAACTAAAACCAGGTGGGTGGCTC ACACCAGTGCTCCAAGCACTAAGAATGCTGAAGCAGGAAGATGTCTGAAGCTGAGTCCAGCCTGGGCTGC AGAGTAAGAGGCAGTCTCAGGGCGAAACAAAATAATGTGGGACTTTAGATATTCTAACTCTAATGTATCT CAAGGTTGGGGGTAGCATGCGCACGCACGCACAGAGAGAGAGTTTCCCCCATATAACCCAGTGTGGTCCT GAACTTTTTAGGCCCCCCCCCCATTAGCTTTCCAAGTGCTGGGATTGCAGGTGTTTGCTTTCATGCCTGG ATATTTCTTTGTTCTTAAAACAATTTCTTGGGTCTTTACTCTCCCCTTAAAAATCATTTTTATACAATAA ATCTCATCCTTCATGGTTCTATGGTTCTGTAAGTTTAGACACCTTTTTTTTTTTTTTTTTTTTTCCGAGA CAGGGTTTCTCTGTGTAATCCTGGCTGTCCTGGAACTCACCAGGCTGGCCTCGAACTCAGAAATCCGCCT GCCTCTGCCTCCCAAGTGCTGGGATTAAAGGCGTGCGCCACCACGCCCGGTTATTTAGACACCTTTTAAC AGGGGTGGCCTGCCATCATAATCAAGATAAGAGAATTTTGTGGCATTTCCCCAAACTTATTGTGTCCTCT TGTAGTATAGTCCTGCCCCCATCCTCAAATCCTAGCAGGCACAGACCTCTTTCCTGTTCCTTAATATAAG TGTCGTAAGTCACACGGGGGCTTATTTTATGGCATTAAGCTTTTAGCTTTTTCCTTTTAGATCTTACATT GAAGTTAGATGCAGTTGTATAAGCTCGGATAAATGGATAAAGCCGAGTAATCATTAATACAGGACAGAAG AGTTCTATTAGCCTCTCCTTTACCATTCCTGGTAATTTATAGTCAAACCTTGTTCTCTCCCTGACAGTCA CGGACTTGTGCTTTCACTTTATGGCTTGTCTTTTGAAAAGAGTTGTAAGTGGAATTTAATAGTATGTAAC TTTTGAGATTAATGTGTTATAAATTATAGACTTGAGATCCATATCACTGGATGTATCAATAGTTTGATTT CTGTTTCATATGCTATTGTGTGGATGTCTTATTGCTTATTCAACCCTAAGTTGGATTACATTCTTTACAG TTGAGGAGGAAGTTATAAATAAAGCTGCTAAAAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6834283 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6834283 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13201 same embryo
	EMAGE:13202 same embryo
	EMAGE:13200 same embryo
	EurExpress:euxassay_017367 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source